Microchannel type rapid biology microparticle counting method

A counting method and micro-channel technology, applied in the field of biological analysis, can solve the problems of low work efficiency, high cost, large human error, etc., and achieve the effect of simple counting method

Inactive Publication Date: 2009-07-29
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

[0003] The purpose of the present invention is to provide a microchannel fast biological particle counting method to sol

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  • Microchannel type rapid biology microparticle counting method

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[0014] Hela (about 10 6 One / bottle of Hela cells) were suspended in 200 μL of 10% formaldehyde solution by mass percentage for 4 hours of immobilization treatment. After immobilization, the cells were washed twice with phosphate buffered saline to remove excess formaldehyde. Dilute the above-mentioned immobilized Hela cells with 50mM phosphate buffer solution. The dilution ratio is based on adding 20 times the volume of the solution to 1 volume of cell particles. After the diluted micro-flow syringe pump is sucked, under the push of the syringe pump, Cells are pushed into a capillary channel and passed one by one by a UV detector at a detection wavelength of 214nm. When the driving flow rate of the syringe pump was 0.20 μL / min, 120 cell peaks were detected within 17 minutes, and the calculated concentration was 3×10 4 pieces / ml. When the flow rate is driven by a syringe pump of 0.14 μl / ml, 100 cell peaks are detected within 24 minutes, and the calculated concentration is 3×...

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Abstract

The invention relates to a micro-channel rapid biological particles counting method and belongs to the bioanalysis field. The method comprises the following steps: the biological particles to be detected are fixed and pass a micro-channel tube line at a constant flow rate, absorption peak number is recorded by a UV-VIS optical detector and the concentration C biological particles (number/ml) of the particle solution is computed, and the C biological particles equal N/ST, wherein, T is the detection time, N is the peak number recorded within T, and S is the flow rate of a pump. The biological particles counting method is simple and rapid, and can accurately compute cell concentration and cell count, which causes the traditional artificial cell activity observation and judgment to obtain a magnitude standard and helps realize quantitative analysis.

Description

technical field [0001] The invention relates to a microchannel type fast biological particle counting method, which belongs to the field of biological analysis. Background technique [0002] Biological particle counting is an extremely important and indispensable statistical and characterization method for the "amount" of biological particles in the research fields of cell biology, microbiology, molecular biology and toxicology. The conventional method of counting cells is using a cell counter and microscope. The basic method is: prepare cell suspension, drop it on a counting plate (a special thick glass slide with a counting pool on it, and there are straight lines at the bottom of the pool to divide the counting pool into many small squares), cover it with a cover glass, and then Directly count one or more types of cells in several small squares (known volume) with the naked eye under a microscope, so that the number or number ratio of these cells in a unit volume of cell...

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Application Information

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IPC IPC(8): G01N15/06G01N21/33G01N27/02G01N21/64
Inventor 屈锋任肖敏
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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