Microchannel type rapid biology microparticle counting method
A counting method and micro-channel technology, applied in the field of biological analysis, can solve the problems of low work efficiency, large human error and high cost, and achieve the effect of simple counting method and quantitative analysis.
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[0014] Hela (about 10 6 One / bottle of Hela cells) were suspended in 200 μL of 10% formaldehyde solution by mass percentage for 4 hours of immobilization treatment. After immobilization, the cells were washed twice with phosphate buffered saline to remove excess formaldehyde. Dilute the above-mentioned immobilized Hela cells with 50mM phosphate buffer solution, and the dilution ratio is based on adding 20 times the volume of solution to 1 volume of cells. After the diluted micro-flow syringe pump is sucked, under the push of the syringe pump, the Cells are pushed into a capillary channel and passed one by one by a UV detector at a detection wavelength of 214nm. When the driving flow rate of the syringe pump was 0.20 μL / min, 120 cell peaks were detected within 17 minutes, and the calculated concentration was 3×10 4 pieces / ml. When the flow rate is driven by a syringe pump of 0.14 μl / ml, 100 cell peaks are detected within 24 minutes, and the calculated concentration is 3×10 4...
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