Reagent combination and kit for extracting nucleic acid by paramagnetic particle method and application of reagent combination and kit

By combining tri(2-formylethyl)phosphine hydrochloride and heavy sulfite as antioxidants, the existing RNA extraction reagents are solved, and efficient and non-toxic nucleic acid extraction is achieved, which is suitable for multiple types of samples.

CN119639732APending Publication Date: 2025-03-18WUHAN MGI TECH CO LTD
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Patent Information

Application Number
CN202311203513.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-09-15
Publication Date
2025-03-18

AI Technical Summary

Technical Problem

Existing RNA extraction reagents have problems such as long time, low purity, low yield and extremely easy extraction failure. Traditional reagents use volatile toxic reagents or chemical reagents that need to be stored at low temperature, which affects the safety of operation and the suitability and stability of the reagents.

Method used

A combination of reagents for extracting nucleic acids by magnetic beads, including lysate and detergent solution, using tris(2-formylethyl)phosphine hydrochloride and heavy sulfite as antioxidants, replacing traditional antioxidants with irritating odors or needing to be stored at low temperatures, and combining magnetic bead technology to achieve efficient and non-toxic nucleic acid extraction.

Benefits of technology

It achieves high yield, high purity and fragment integrity RNA extraction, suitable for multiple types of samples, is non-toxic and can be stored at room temperature, improving extraction efficiency and safety.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a reagent combination and a kit for extracting nucleic acid through a paramagnetic particle method and application of the reagent combination and the kit. The reagent combination or the kit does not adopt beta-mercaptoethanol with pungent smell or dithiothreitol needing to be stored at low temperature as an antioxidant, but adopts tris (2-formylethyl) phosphine hydrochloride and / or metabisulfite as an antioxidant, belongs to common salt chemical reagent powder, can be stored at normal temperature, and can be used for preparing a reagent for the first time. Manual addition by experimenters before an experiment is not needed, so that nucleic acid (RNA) released by splitting decomposition can be effectively prevented from being oxidized and denatured, and the integrity of extracted nucleic acid (total RNA) fragments is ensured; especially, the metabisulfite is commonly used in food additives, does not cause harm to the body, and is non-toxic and non-volatile.
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