Method for breaking walls of Haematococcus pluvialis and extracting astaxanthin

A technology of Haematococcus pluvialis and Haematococcus pluvialis powder, applied in the direction of organic chemistry, can solve the problems of loss, high temperature selection, cumbersome steps, etc., reduce loss and denaturation, mild reaction conditions, and simple operation steps Effect

Active Publication Date: 2017-08-04
GUOTOU BIO TECH INVESTMENT CO LTD +1
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  • Abstract
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Problems solved by technology

However, in this method, the cell wall breaking temperature is still high, resulting in the instability of astaxanthin and the loss of active ingredients; in addition, there are "adjusting the pH to neutral with 10% NaOH" and "adding 95% ethanol for dehydration" in this method. The processing steps, the extraction process requires many kinds of reagents, the consumption is large, and the operation steps are cumbersome, which directly affects the extraction cost; and the content of astaxanthin in the extract obtained by this method is low, only about 6%, and the impurities are relatively increased. Increased the difficulty of subsequent purification
[0006] In summary, the main problems in the prior art include high selection temperature, large amount of solvent, low content of astaxanthin in the extract, complex operation, cumbersome steps, time-consuming, energy-consuming, expensive equipment and relatively low overall cost. higher

Method used

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  • Method for breaking walls of Haematococcus pluvialis and extracting astaxanthin

Examples

Experimental program
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Embodiment 1

[0037] Weigh 200 mg of algae powder, put it in a reaction flask, add 5% sulfuric acid-methanol system (volume fraction, v / v) at a liquid-to-solid ratio of 10:1 (mL / g), and stir at 50 ° C for 1 h to break the wall. After the wall-breaking treatment of 5% sulfuric acid-methanol system, the extraction solvent n-hexane was added according to the liquid-to-solid ratio of 10:1 (mL / g), stirred and extracted at 30°C for 30 minutes, and the supernatant was collected by centrifugation and layered with liquid nitrogen. Blow dry and concentrate to obtain an extract rich in astaxanthin. The content of astaxanthin is determined by high performance liquid chromatography. The content of astaxanthin in the extract is 10.2%, and the extraction rate of astaxanthin is calculated to be 93.5%. It can be seen that the content of astaxanthin is 10.2%, and the content of astaxanthin in the crude extract has exceeded 10%, so the difficulty of subsequent purification can be greatly reduced, and it is mor...

Embodiment 2

[0039] Weigh 200 mg of algae powder, put it in a reaction flask, add 3% sulfuric acid-methanol system (volume fraction, v / v) at a liquid-to-solid ratio of 10:1 (mL / g), and stir at 30 ° C for 1 h to break the wall. After being treated with 3% sulfuric acid-methanol system for wall breaking, add the extraction solvent n-hexane at a liquid-to-solid ratio of 10:1 (mL / g), stir and extract at 70°C for 30 minutes, centrifuge and separate to obtain the supernatant, supernatant liquid nitrogen Dried and concentrated to obtain an extract rich in astaxanthin, the content of astaxanthin was determined by high performance liquid chromatography, and the content of astaxanthin in the extract was 10.3%, and the calculated astaxanthin extraction rate was 92.3%. The result of this embodiment is basically consistent with that of embodiment 1. The content of astaxanthin is 10.3%, and the content of astaxanthin in its crude extract has exceeded 10%. Therefore, the method for breaking the wall of ...

Embodiment 3

[0041] Weigh 200 mg of algae powder, put it in a reaction flask, add 10% sulfuric acid-methanol system (volume fraction, v / v) at a liquid-to-solid ratio of 10:1 (mL / g), and stir at 70 ° C for 1 h to break the wall. After breaking the wall with 10% sulfuric acid-methanol, add a vegetable oil extraction solvent as the extraction solvent according to the liquid-to-solid ratio of 10:1 (mL / g), stir and extract at 50°C for 30 minutes, centrifuge and separate the supernatant, and The clear liquid was blown dry with nitrogen gas, concentrated to be rich in astaxanthin extract, and the content of astaxanthin was determined by high performance liquid chromatography. The content of astaxanthin in the extract was 9.6%, and the calculated astaxanthin extraction rate was 87.5%. The content of astaxanthin in the crude extract is close to 10%.

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Abstract

The invention relates to a method for breaking walls of Haematococcus pluvialis and extracting astaxanthin. The method comprises the following steps: (S1) uniformly mixing Haematococcus pluvialis powder with a concentrated sulfuric acid-alcohol system, stirring at 30-70 DEG C, and carrying out wall breaking for a preset time, so as to obtain an alcohol solution containing wall-broken Haematococcus pluvialis; and (S2) adding an extraction solvent into the alcohol solution containing the wall-broken Haematococcus pluvialis obtained in the step (S1), carrying out stirring extraction at 30-70 DEG C, layering, and concentrating supernate, so as to obtain an extract containing the astaxanthin. The method has the beneficial effects that the wall-breaking temperature and the extraction temperature are relatively low, reaction conditions are mild, the loss of astaxanthin is reduced, and the content of the astaxanthin in a crude extract can reach up to about 10%, so that the subsequent purification level of difficulty can be lowered, and the commercialization of the astaxanthin is promoted. Compared with existing methods, the method has the advantages that the operation steps are relatively simplified and the type and use amount of reagents are less, so that the energy consumption can be reduced, and the comprehensive cost can be lowered.

Description

technical field [0001] The invention relates to the field of extracting algae functional components, in particular to a method for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Astaxanthin (3,3′-dihydroxy-4,4′-diketo-β,β-carotene) is an oxygenated derivative of carotenoids and belongs to the group of ketocarotenoids [1] , natural astaxanthin is synthesized by algae, plants, bacteria and fungi. Astaxanthin is a conjugated molecule composed of 13 conjugated double bonds. The alternation of single and double bonds increases the antioxidant capacity of astaxanthin, which can neutralize free radicals or scavenge active oxygen, and can prevent diseases caused by free radicals, including oral ulcers , colon cancer, cardiovascular disease, etc., and can prevent eye diseases such as cataracts, and can be used in food, medicine, cosmetics and other fields. In addition, astaxanthin can also be used as a colorant in aquaculture [2] . [0003] Asta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 杨小红王贵春韩丹翔胡强
Owner GUOTOU BIO TECH INVESTMENT CO LTD
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