Method for breaking walls of Haematococcus pluvialis and extracting astaxanthin

Abstract

The invention relates to a method for breaking walls of Haematococcus pluvialis and extracting astaxanthin. The method comprises the following steps: (S1) uniformly mixing Haematococcus pluvialis powder with a concentrated sulfuric acid-alcohol system, stirring at 30-70 DEG C, and carrying out wall breaking for a preset time, so as to obtain an alcohol solution containing wall-broken Haematococcus pluvialis; and (S2) adding an extraction solvent into the alcohol solution containing the wall-broken Haematococcus pluvialis obtained in the step (S1), carrying out stirring extraction at 30-70 DEG C, layering, and concentrating supernate, so as to obtain an extract containing the astaxanthin. The method has the beneficial effects that the wall-breaking temperature and the extraction temperature are relatively low, reaction conditions are mild, the loss of astaxanthin is reduced, and the content of the astaxanthin in a crude extract can reach up to about 10%, so that the subsequent purification level of difficulty can be lowered, and the commercialization of the astaxanthin is promoted. Compared with existing methods, the method has the advantages that the operation steps are relatively simplified and the type and use amount of reagents are less, so that the energy consumption can be reduced, and the comprehensive cost can be lowered.

Description

technical field [0001] The invention relates to the field of extracting algae functional components, in particular to a method for extracting astaxanthin from Haematococcus pluvialis. Background technique [0002] Astaxanthin (3,3′-dihydroxy-4,4′-diketo-β,β-carotene) is an oxygenated derivative of carotenoids and belongs to the group of ketocarotenoids [1] , natural astaxanthin is synthesized by algae, plants, bacteria and fungi. Astaxanthin is a conjugated molecule composed of 13 conjugated double bonds. The alternation of single and double bonds increases the antioxidant capacity of astaxanthin, which can neutralize free radicals or scavenge active oxygen, and can prevent diseases caused by free radicals, including oral ulcers , colon cancer, cardiovascular disease, etc., and can prevent eye diseases such as cataracts, and can be used in food, medicine, cosmetics and other fields. In addition, astaxanthin can also be used as a colorant in aquaculture [2] . [0003] Asta...

AI Technical Summary

Problems solved by technology

However, in this method, the cell wall breaking temperature is still high, resulting in the instability of astaxanthin and the loss of active ingredients; in addition, there are "adjusting the pH to neutral with 10% NaOH" and "adding 95% ethanol for dehydration" in this method. The processing steps, the extraction process requires many kinds of reagents, the consumption is large, and the operation steps are cumbersome, which directly affects the extraction cost; and the content of astaxanthin in the extract obtained by this method is low, only about 6%, and the impurities are relatively increased. Increased the difficulty of subsequent purification

[0006] In summary, the main problems in the prior art include high selection temperature, large amount of solvent, low content of astaxanthin in the extract, complex operation, cumbersome steps, time-consuming, energy-consuming, expensive equipment and relatively low overall cost. higher

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