38results about How to "No join" patented technology

The invention belongs to the technical field of microalgae microorganisms and relates to a wall-breaking method for haematococcus pluvialis cells which are rich in a biological active substance: astaxanthin. The wall-breaking method for the haematococcus pluvialis cells provided by the invention comprises the following three steps: 1, freezing and ice-crystallizing the haematococcus pluvialis cells and dispersing the cells in a flowing liquid below 0 DEG C; 2, adding a bioactive component which protects the haematococcus pluvialis into the liquid before the ice-crystallized algae cells are broken so as to reduce a protectant which is oxidized and decomposed after wall breaking; and 3, after uniformly grinding by a colloid mill, carrying out ultra-high-pressure wall-breaking by using an ultra-high-pressure homogeneous nanometer machine. The wall-breaking process provided by the invention is carried out under a condition of low-temperature, cold chain and close away from light and breaks walls by means of the characteristic of nanometer crushing by using the ultra-high-pressure homogeneous nanometer machine and has the advantages of rapidness, efficiency and no addition of foreign matters and can be used for effectively protecting the bioactive component.

The invention discloses a method for separating the tissue cell of a fetal appendage and constructing a cell bank by means of a tissue homogenate method, so that the process for constructing the cell blank is simple in steps, time-saving, and capable of achieving easy quality control and standardization. The method disclosed by the invention comprises the following steps of (1) cleaning the tissue of the fetal appendage; (2) carrying out homogenate pretreatment on the tissue; (3) carrying out homogenate treatment on the tissue to separate a cell (cell cluster); (4) filtering the tissue and collecting the cell (cell cluster) after the homogenate; (5) detecting the cell (cell cluster); (6) cryopreserving the separated cell (cell cluster) and constructing the cell bank. Compared with the method in the prior art, the method disclosed by the invention has the advantages of simple operation, short time consumption, no introduction of an exogenous reagent, easiness for standardization, easy quality control, great cell gain and the like, and is of great significance in improving the construction efficiency of the fetal appendage cell bank and obtaining the stem cell which is high in quality and applied to treatment.

The invention discloses a method for preparing a high-dispersibility nano Pt-SnO2/C catalyst. Carbon black loaded nano tin dioxide and loaded platinum-based tin dioxide binary composite catalyst is prepared by an ultrasonic-assisted method, and the prepared catalyst has high electrocatalytic activity and durability on oxygen reduction reaction under the acidic condition. Compared with a platinum/carbon black catalyst, the high-dispersibility nano Pt-SnO2/C catalyst has the characteristics of low price, stable performance and the like.

The invention discloses a quaternization xylan, preparation method via a semi-dry process and an application of the quaternization xylan. The preparation method disclosed by the invention comprises the following steps of: firstly, dissolving xylan in an alkaline solution and alkalinizing for 10-40 minutes at temperature of 20-40DEG C; adding the acquired mixture solution into a quaternization reagent, uniformly mixing, placing in a drying oven and reacting for 2-10 hours at temperature of 50-90DEG C, wherein the mass ratio of the quaternization reagent to the xylan is (1-8):1; and cooling the acquired product, washing the product by using lower alcohol, subsequently filtering and drying to acquire the quaternization xylan, wherein the lower alcohol is methyl alcohol, ethanol or propyl alcohol. The preparation method disclosed by the invention is simple in process, easily controllable in reaction conditions, good in operability and low in cost; and the prepared quaternization xylan has a degree of substitution of 0.10-0.60 and a molecular weight of 21730-32728g/mol, and can be applied to papermaking and pharmaceuticals industries as a papermaking additive and a DNA (Deoxyribonucleic Acid) vector.

The invention relates to a manufacturing method of a Tibetan decorative brick tea which is manufactured by the processes of collecting tea, detecting, deactivation of enzymes, fermentation, drying, watering and stirring, pressing in a mold, drying again preparing for a bracket and packing. The brick tea of the invention has the advantages of low-temperature natural manufacturing technique, no chemical additive and being capable of maintaining the helpful substance in the tea; a prepared brick tea is convenient for storing, carrying and drinking, which can emit tea perfume into a room, thus improving indoor air and decorating the environment.

The invention discloses a preparation method of resin carbon for blood purification. The preparation method comprises the following steps: preparing chloromethylated styrene-divinylbenzene microspheres from p-chloromethyl styrene monomers and divinylbenzene monomers; preparing polystyrene-divinylbenzene microspheres from the chloromethylated styrene-divinylbenzene microspheres; dipping the polystyrene-divinylbenzene microspheres by using a zinc-chloride solution to obtain dipped microspheres; and carbonizing the dipped microspheres, and activating the dipped microspheres after the carbonizing so as to obtain a finished product. The preparation method disclosed by the invention has the obvious effects that the resin carbon with the excellent absorbing effect for middle molecules can be prepared and obtained at relatively low temperature and within shorter time, the preparation process is simple, the pollution is slight, the product yield is high and the purity is high.

The present invention provides a muscular amino acids and peptides and nucleosides injection, which is extracted from the muscle and the cardiac muscle of healthy rabbit and is a freeze-drying agent which is prepared by the sterilized water solution containing the compositions of polypeptide, amino acids, nucleosides, nucleotides, etc. The muscular amino acids and peptides and nucleosides is used for the adjuvant therapy of brain hypofunction and peripheral nerve disease caused by cerebral apoplexy and cerehral circulation insufficiency. The present invention belongs to the technical field of medicine. The invention introduces a preparing method which comprises the steps of cleaning, cutting into blocks, homogenizing, deep freezing, heating to boiled, separating the supernatant, depositing with acid and the base, depositing with the base, hot pressing, deep freezing, ultra-filtering, detecting, adjusting the proportion between the polypeptide and the hypoxanthine, and ultra-filtering. The muscular amino acids and peptides and nucleosides injection is prepared through the steps of sterilizing filtering, filling into containers, and sterilizing. An appropriate amount of accessory is added and the steps of sterilizing filtering, filling into containers, and freeze drying are executed for preparing the muscular amino acids and peptides and nucleosides for injection. The muscular amino acids and peptides and nucleosides and the preparing method of the invention have the advantages of excellent convenience, excellent wholeness, ensured product which satisfies the national standard, guaranteed virus blanching, injection sterilization F0 larger than 8, and high level of sterilizing guaranteeing.

The invention discloses a cation-doped and modified ternary positive electrode material for a lithium ion battery and a preparation method of the ternary positive electrode material, and relates to the technical field of positive electrode materials for lithium ion batteries. The cation-doped and modified ternary positive electrode material is obtained by uniformly mixing nanoscale metal oxide particles with a ternary material precursor and lithium salt and then roasting at high temperature. The invention also discloses a preparation method of the cation-doped and modified ternary positive electrode material for the lithium ion battery, which comprises the following steps: stirring and mixing the nano-scale metal oxide and the ternary positive electrode material precursor in a solvent to obtain a precursor material uniformly coated with the nano-scale metal oxide; and drying, uniformly mixing with a lithium salt, and roasting to obtain the modified ternary positive electrode material with uniformly doped cations. The ternary positive electrode material obtained by the method shows more excellent cycling stability and rate capability than those of an undoped modified material under the high voltage of 4.5 V, plays an important role in inhibiting cation mixing, collapse of a layered structure and lattice oxygen release, and can greatly improve the electrochemical performance of the material.

The invention provides a rare earth oxide coated and modified ternary positive electrode material for a lithium ion battery and a preparation method of the rare earth oxide coated and modified ternary positive electrode material and belongs to the technical field of lithium ion battery positive electrode materials. The rare earth oxide coated and modified ternary positive electrode material is obtained by uniformly mixing nanoscale rare earth oxide particles with a ternary material precursor and lithium salt and then roasting an obtained mixture at high temperature. The invention also discloses a preparation method of the rare earth oxide coated and modified ternary positive electrode material for the lithium ion battery. The rare earth oxide coated and modified ternary positive electrode material obtained by the method shows more excellent charge-discharge cycle stability and rate capability than a ternary material which is not coated and modified at a high voltage of 4.5 V; and the rare earth oxide coated and modified ternary positive electrode material can be widely applied to lithium ion battery positive electrode materials and is suitable for popularization and application.

The invention relates to a concentrated freeze-dried flower and fruit tea, and a preparation method and a drinking method thereof, and belongs to the technical field of substitutional tea. The concentrated freeze-dried flower and fruit tea is prepared from, by weight, fresh peeled lemons, fresh sweet osmanthus flowers, passion fruit pulp, honey powder and the like. The preparation method of the concentrated freeze-dried flower and fruit tea comprises the following steps: S1, preparing the raw materials in parts by weight of the concentrated freeze-dried flower and fruit tea; S2, mixing the prepared raw materials, juicing for several minutes, and pouring into a container; S3, placing the container in a freeze dryer, and freeze-drying for several hours; and S4, packaging the prepared concentrated freeze-dried flower and fruit tea into bags. The concentrated freeze-dried flower and fruit tea can be drunk or directly eaten. According to the concentrated freeze-dried flower and fruit tea, the freeze-drying technology is adopted, so the color, fragrance, taste and nutritional ingredients of fresh fruits and raw materials are effectively reserved, the water content is extremely low, and the shelf life of the product is greatly prolonged.

The invention discloses a sweet potato nutrition silk noodle and a preparation method thereof. The preparation method comprises the following steps of (1) material selection; (2) cleaning; (3) peeling; (4) pulping: peeled sweet potatoes are subjected to extrusion crushing; the extrusion crushing speed of the peeled sweet potatoes is 10 to 100kg/min; color protection liquid is added at a speed of 20 to 200kg/min in the extrusion crushing process; the mixture passes through a 60-100 mesh sieve to obtain sweet potato raw pulp; (5) primary separation; (6) refined filtration; (7) secondary separation; (8) sweet potato nutrition silk noodle preparation. According to the sweet potato nutrition silk noodle and the preparation method of the sweet potato nutrition silk noodle disclosed by the invention, the sweet potato nutrition silk noodle is prepared by using non-transgenic sweet potatoes as raw materials through material selection, cleaning, peeling, pulping, primary separation, refined filtration and secondary separation. Compared with the prior art, the pulping process has the advantages that the color protection liquid is used for enzymolysis and color protection fresh keeping; the fast, efficient and foreign-matter-free addition is realized; the biological active ingredients can be effectively protected. The sweet potato nutrition silk noodle is favorably absorbed by the human body; the nutrition and health care values are high.

The invention relates to a formamide preparation method. The method allows the product formamide to prepared from a formylating reagent and amine (primary amine or secondary amine) through a formylating reaction under the promotion of an acidic gas. The method is characterized in that the formylating reaction is efficiently carried out under mild conditions with the acidic gas as a reaction promoter. The method comprises the following steps: mixing a certain concentration of the formylating reagent with the primary amine or secondary amine without adding other solvents, introducing a certain amount of the acidic gas, sealing, stirring at a temperature higher than room temperature, and reacting for 0.5h or more to obtain the product formamide. The method has the advantages of high controllability and no addition of a catalyst in the reaction process; the promoter is a gas and rapidly volatilizes when the reaction ends, so separation is not needed; the yield of the product formamide reaches above 95%; and the promoter has very good substrate universality.

The invention belongs to the technical field of cellulose materials, and relates to a preparation method of cellulose-based foaming buffer packaging materials in a BmimCl/LiOH system. The preparationmethod includes the steps: performing vacuum drying on microcrystalline cellulose; taking mixed solution of chlorination-1-butyl-3-methylimidazole and lithium hydroxide solution as a solvent, adding the dried microcrystalline cellulose, and uniformly mixing mixture; leading in shielding gas, and performing anti-oxidation decomposition treatment; performing stirring and heating to obtain microcrystalline cellulose solution; dripping the solution into water by a falling-ball method to obtain cellulose hydrogel; washing the cellulose hydrogel by a gradient washing method; performing vacuum freezedrying to obtain porous cellulose-based foaming buffer packaging materials; performing filtration, purification and rotary evaporation on the residual solvent, and recycling ionic liquid. The prepared cellulose packaging materials can be biodegraded and are environmentally friendly, a preparation process of the cellulose-based foaming buffer packaging materials is simplified, chemical additivesare omitted, and the packaging materials has the advantages that the materials are environmentally friendly and free from pollution.

The invention discloses sweet potato powder capable of maintaining beauty and slimming a body and a making method thereof. The making method comprises the following steps that 1, sweet potatoes are cleaned and peeled and then cut into slices and blocks with the particle size of 1-15 mm to obtain sweet potato strips, the sweet potato strips are soaked in color protecting liquid to be subjected to color protecting for 10 min to 40 min, filtering is conducted, and a color protecting product is obtained; 2, the color protecting product is put into enzyme liquid at the temperature of 30 DEG C to 40 DEG C to be subjected to enzymolysis for 1 h to 4 h, filtering is conducted, and an enzymolysis product is obtained; 3, the enzymolysis product is dried for 5 h to 15 h at the temperature of 50 DEG C to 80 DEG C and then crushed to be 100 mesh to 500 mesh. The sweet potato powder is made by carefully selecting the fresh sweet potatoes as raw materials through the steps of cleaning, cutting to strips, color protecting, enzymolysis conducting, drying and crushing. Compared with the prior art, the sweet potato powder has the advantages that rapidness and high efficiency are achieved, no foreign matter is added, and bioactive components can be effectively protected and is more beneficial for human body absorption and high in nutritional health-care value.