38results about How to "No join" patented technology

The invention belongs to the technical field of microalgae microorganisms and relates to a wall-breaking method for haematococcus pluvialis cells which are rich in a biological active substance: astaxanthin. The wall-breaking method for the haematococcus pluvialis cells provided by the invention comprises the following three steps: 1, freezing and ice-crystallizing the haematococcus pluvialis cells and dispersing the cells in a flowing liquid below 0 DEG C; 2, adding a bioactive component which protects the haematococcus pluvialis into the liquid before the ice-crystallized algae cells are broken so as to reduce a protectant which is oxidized and decomposed after wall breaking; and 3, after uniformly grinding by a colloid mill, carrying out ultra-high-pressure wall-breaking by using an ultra-high-pressure homogeneous nanometer machine. The wall-breaking process provided by the invention is carried out under a condition of low-temperature, cold chain and close away from light and breaks walls by means of the characteristic of nanometer crushing by using the ultra-high-pressure homogeneous nanometer machine and has the advantages of rapidness, efficiency and no addition of foreign matters and can be used for effectively protecting the bioactive component.

The invention discloses a method for separating the tissue cell of a fetal appendage and constructing a cell bank by means of a tissue homogenate method, so that the process for constructing the cell blank is simple in steps, time-saving, and capable of achieving easy quality control and standardization. The method disclosed by the invention comprises the following steps of (1) cleaning the tissue of the fetal appendage; (2) carrying out homogenate pretreatment on the tissue; (3) carrying out homogenate treatment on the tissue to separate a cell (cell cluster); (4) filtering the tissue and collecting the cell (cell cluster) after the homogenate; (5) detecting the cell (cell cluster); (6) cryopreserving the separated cell (cell cluster) and constructing the cell bank. Compared with the method in the prior art, the method disclosed by the invention has the advantages of simple operation, short time consumption, no introduction of an exogenous reagent, easiness for standardization, easy quality control, great cell gain and the like, and is of great significance in improving the construction efficiency of the fetal appendage cell bank and obtaining the stem cell which is high in quality and applied to treatment.

The invention relates to a preparation method of a high oil-carrying emulsion rich in fibrous polysaccharides. The preparation method comprises the following steps: protein powder is taken and added into distilled water, and is magnetically stirred and dissolved at a normal temperature to prepare a protein solution by hydration in a refrigerator at 4 DEG C; the protein solution and fibrous polysaccharide aqueous dispersion powder are mixed, and the pH value of the system is adjusted to obtain a protein / fiber complex; and an oil phase is dispersed in the protein / fiber complex and emulsificationis performed to prepare an oil-in-water emulsion. According to the preparation method of the high oil-carrying emulsion rich in the fibrous polysaccharides, the protein and fibrous polysaccharide complex is used as an emulsifying stabilizer to synergistically improve the wettability and stability of particles, and proteins and fibrous polysaccharides jointly adsorb on an oil-water interface to form a stable three-dimensional network structure to obtain the viscoelastic and highly stable emulsion. Natural food grade materials are adopted. The preparation process is simple and convenient, no surfactant is added, and green and safety are achieved. The content of the required compound stabilizer is low, and good emulsification stability is achieved. The oil phase and a water phase are removedfrom the prepared oil-in-water emulsion, a porous and stable network structure can be formed, and the high oil-carrying emulsion can be used as a porous polymer scaffold material in tissue engineering.

The invention discloses a method for preparing a high-dispersibility nano Pt-SnO2 / C catalyst. Carbon black loaded nano tin dioxide and loaded platinum-based tin dioxide binary composite catalyst is prepared by an ultrasonic-assisted method, and the prepared catalyst has high electrocatalytic activity and durability on oxygen reduction reaction under the acidic condition. Compared with a platinum / carbon black catalyst, the high-dispersibility nano Pt-SnO2 / C catalyst has the characteristics of low price, stable performance and the like.

The invention discloses a quaternization xylan, preparation method via a semi-dry process and an application of the quaternization xylan. The preparation method disclosed by the invention comprises the following steps of: firstly, dissolving xylan in an alkaline solution and alkalinizing for 10-40 minutes at temperature of 20-40DEG C; adding the acquired mixture solution into a quaternization reagent, uniformly mixing, placing in a drying oven and reacting for 2-10 hours at temperature of 50-90DEG C, wherein the mass ratio of the quaternization reagent to the xylan is (1-8):1; and cooling the acquired product, washing the product by using lower alcohol, subsequently filtering and drying to acquire the quaternization xylan, wherein the lower alcohol is methyl alcohol, ethanol or propyl alcohol. The preparation method disclosed by the invention is simple in process, easily controllable in reaction conditions, good in operability and low in cost; and the prepared quaternization xylan has a degree of substitution of 0.10-0.60 and a molecular weight of 21730-32728g / mol, and can be applied to papermaking and pharmaceuticals industries as a papermaking additive and a DNA (Deoxyribonucleic Acid) vector.

The invention relates to a manufacturing method of a Tibetan decorative brick tea which is manufactured by the processes of collecting tea, detecting, deactivation of enzymes, fermentation, drying, watering and stirring, pressing in a mold, drying again preparing for a bracket and packing. The brick tea of the invention has the advantages of low-temperature natural manufacturing technique, no chemical additive and being capable of maintaining the helpful substance in the tea; a prepared brick tea is convenient for storing, carrying and drinking, which can emit tea perfume into a room, thus improving indoor air and decorating the environment.

The invention discloses a method for preparing a polyurethane-modified organosilicon double-component room-curing elastomer adhesive, which comprises the following steps: (1) uniformly mixing hydroxypropyl-terminated silicone oil and glycerol, and dehydrating to obtain a hydroxyl-terminated (-OH) component; (2) reacting aromatic polyisocyanate, the hydroxyl-terminated (-OH) component and dibutyltin dilaurate to obtain a component A; (3) adding vapor-phase silica white, carbon black and talcum powder into the container filled with hydroxy silicone oil and dihydroxy polysiloxane, adding a catalyst, adding 3-aminopropyl triethoxy silane, finally adding a defoaming agent, uniformly mixing, and drying to obtain a component B; and (4) uniformly mixing the component A and component B in a mass ratio of 12:(23.5-24), and curing at room temperature for 4-5 hours. The elastomer adhesive prepared by the method has the advantages of favorable tear resistance, excellent high / low temperature resistance and excellent binding property. The preparation method is easy to operate and accessible in raw materials, and lowers the preparation cost.

The invention discloses a preparation method of resin carbon for blood purification. The preparation method comprises the following steps: preparing chloromethylated styrene-divinylbenzene microspheres from p-chloromethyl styrene monomers and divinylbenzene monomers; preparing polystyrene-divinylbenzene microspheres from the chloromethylated styrene-divinylbenzene microspheres; dipping the polystyrene-divinylbenzene microspheres by using a zinc-chloride solution to obtain dipped microspheres; and carbonizing the dipped microspheres, and activating the dipped microspheres after the carbonizing so as to obtain a finished product. The preparation method disclosed by the invention has the obvious effects that the resin carbon with the excellent absorbing effect for middle molecules can be prepared and obtained at relatively low temperature and within shorter time, the preparation process is simple, the pollution is slight, the product yield is high and the purity is high.

The present invention provides a muscular amino acids and peptides and nucleosides injection, which is extracted from the muscle and the cardiac muscle of healthy rabbit and is a freeze-drying agent which is prepared by the sterilized water solution containing the compositions of polypeptide, amino acids, nucleosides, nucleotides, etc. The muscular amino acids and peptides and nucleosides is used for the adjuvant therapy of brain hypofunction and peripheral nerve disease caused by cerebral apoplexy and cerehral circulation insufficiency. The present invention belongs to the technical field of medicine. The invention introduces a preparing method which comprises the steps of cleaning, cutting into blocks, homogenizing, deep freezing, heating to boiled, separating the supernatant, depositing with acid and the base, depositing with the base, hot pressing, deep freezing, ultra-filtering, detecting, adjusting the proportion between the polypeptide and the hypoxanthine, and ultra-filtering. The muscular amino acids and peptides and nucleosides injection is prepared through the steps of sterilizing filtering, filling into containers, and sterilizing. An appropriate amount of accessory is added and the steps of sterilizing filtering, filling into containers, and freeze drying are executed for preparing the muscular amino acids and peptides and nucleosides for injection. The muscular amino acids and peptides and nucleosides and the preparing method of the invention have the advantages of excellent convenience, excellent wholeness, ensured product which satisfies the national standard, guaranteed virus blanching, injection sterilization F0 larger than 8, and high level of sterilizing guaranteeing.

The invention discloses a cation-doped and modified ternary positive electrode material for a lithium ion battery and a preparation method of the ternary positive electrode material, and relates to the technical field of positive electrode materials for lithium ion batteries. The cation-doped and modified ternary positive electrode material is obtained by uniformly mixing nanoscale metal oxide particles with a ternary material precursor and lithium salt and then roasting at high temperature. The invention also discloses a preparation method of the cation-doped and modified ternary positive electrode material for the lithium ion battery, which comprises the following steps: stirring and mixing the nano-scale metal oxide and the ternary positive electrode material precursor in a solvent to obtain a precursor material uniformly coated with the nano-scale metal oxide; and drying, uniformly mixing with a lithium salt, and roasting to obtain the modified ternary positive electrode material with uniformly doped cations. The ternary positive electrode material obtained by the method shows more excellent cycling stability and rate capability than those of an undoped modified material under the high voltage of 4.5 V, plays an important role in inhibiting cation mixing, collapse of a layered structure and lattice oxygen release, and can greatly improve the electrochemical performance of the material.

The invention provides a rare earth oxide coated and modified ternary positive electrode material for a lithium ion battery and a preparation method of the rare earth oxide coated and modified ternary positive electrode material and belongs to the technical field of lithium ion battery positive electrode materials. The rare earth oxide coated and modified ternary positive electrode material is obtained by uniformly mixing nanoscale rare earth oxide particles with a ternary material precursor and lithium salt and then roasting an obtained mixture at high temperature. The invention also discloses a preparation method of the rare earth oxide coated and modified ternary positive electrode material for the lithium ion battery. The rare earth oxide coated and modified ternary positive electrode material obtained by the method shows more excellent charge-discharge cycle stability and rate capability than a ternary material which is not coated and modified at a high voltage of 4.5 V; and the rare earth oxide coated and modified ternary positive electrode material can be widely applied to lithium ion battery positive electrode materials and is suitable for popularization and application.

The invention belongs to the field of electrocatalysts, and particularly relates to a strontium-doped precious metal oxide oxygen evolution electrocatalyst and a preparation method thereof. Accordingto the changing condition of solubility of strontium hydroxide along with temperature, high-temperature strontium hydroxide solution reacts with metal chloric acid, an outer layer of precious metal hydroxide separates out strontium hydroxide precipitates by cooling, and the precipitates are calcined at high temperature to obtain the strontium-doped precious metal oxide oxygen evolution electrocatalyst. The method is simple and low in equipment requirement, and the prepared oxygen evolution electrocatalyst is good in stability and high in catalytic activity, and has more reactive sites.

The invention relates to a concentrated freeze-dried flower and fruit tea, and a preparation method and a drinking method thereof, and belongs to the technical field of substitutional tea. The concentrated freeze-dried flower and fruit tea is prepared from, by weight, fresh peeled lemons, fresh sweet osmanthus flowers, passion fruit pulp, honey powder and the like. The preparation method of the concentrated freeze-dried flower and fruit tea comprises the following steps: S1, preparing the raw materials in parts by weight of the concentrated freeze-dried flower and fruit tea; S2, mixing the prepared raw materials, juicing for several minutes, and pouring into a container; S3, placing the container in a freeze dryer, and freeze-drying for several hours; and S4, packaging the prepared concentrated freeze-dried flower and fruit tea into bags. The concentrated freeze-dried flower and fruit tea can be drunk or directly eaten. According to the concentrated freeze-dried flower and fruit tea, the freeze-drying technology is adopted, so the color, fragrance, taste and nutritional ingredients of fresh fruits and raw materials are effectively reserved, the water content is extremely low, and the shelf life of the product is greatly prolonged.

The invention relates to a processing method of pine resin and belongs to the field of rosin production. The processing method of pine resin comprises the following steps of: a, dissolving, namely dissolving the pine resin by adopting a dissolving solution, wherein the dissolving solution comprises the following components in percentage by weight: 55-82% of turpentine oil, 17.5-44.5% of water and 0.1-0.5% of oxalic acid; b, clarifying and colating, namely placing the dissolved pine resin liquid in a transition pot to be clarified and then colating to obtain colature; c, acid washing, namely adding the oxalic acid for washing; d, washing, namely adding hot water for washing; e, clarifying and carrying out refined filtration, namely placing the pine resin liquid into the transition pot to be clarified and then carrying out refined filtration; and f, heating and distilling, namely placing the pine resin liquid in a rectifying tower to be subjected to the heating and distilling, so that rosin and turpentine oil finished products are respectively obtained. The processing method of rosin provided by the invention has the beneficial effect that the qualities of the rosin and turpentine oil obtained by processing pine resin are excellent, so that demand of downstream industries is met.

The invention discloses a sweet potato nutrition silk noodle and a preparation method thereof. The preparation method comprises the following steps of (1) material selection; (2) cleaning; (3) peeling; (4) pulping: peeled sweet potatoes are subjected to extrusion crushing; the extrusion crushing speed of the peeled sweet potatoes is 10 to 100kg / min; color protection liquid is added at a speed of 20 to 200kg / min in the extrusion crushing process; the mixture passes through a 60-100 mesh sieve to obtain sweet potato raw pulp; (5) primary separation; (6) refined filtration; (7) secondary separation; (8) sweet potato nutrition silk noodle preparation. According to the sweet potato nutrition silk noodle and the preparation method of the sweet potato nutrition silk noodle disclosed by the invention, the sweet potato nutrition silk noodle is prepared by using non-transgenic sweet potatoes as raw materials through material selection, cleaning, peeling, pulping, primary separation, refined filtration and secondary separation. Compared with the prior art, the pulping process has the advantages that the color protection liquid is used for enzymolysis and color protection fresh keeping; the fast, efficient and foreign-matter-free addition is realized; the biological active ingredients can be effectively protected. The sweet potato nutrition silk noodle is favorably absorbed by the human body; the nutrition and health care values are high.

The invention relates to a formamide preparation method. The method allows the product formamide to prepared from a formylating reagent and amine (primary amine or secondary amine) through a formylating reaction under the promotion of an acidic gas. The method is characterized in that the formylating reaction is efficiently carried out under mild conditions with the acidic gas as a reaction promoter. The method comprises the following steps: mixing a certain concentration of the formylating reagent with the primary amine or secondary amine without adding other solvents, introducing a certain amount of the acidic gas, sealing, stirring at a temperature higher than room temperature, and reacting for 0.5h or more to obtain the product formamide. The method has the advantages of high controllability and no addition of a catalyst in the reaction process; the promoter is a gas and rapidly volatilizes when the reaction ends, so separation is not needed; the yield of the product formamide reaches above 95%; and the promoter has very good substrate universality.

The invention belongs to the technical field of cellulose materials, and relates to a preparation method of cellulose-based foaming buffer packaging materials in a BmimCl / LiOH system. The preparationmethod includes the steps: performing vacuum drying on microcrystalline cellulose; taking mixed solution of chlorination-1-butyl-3-methylimidazole and lithium hydroxide solution as a solvent, adding the dried microcrystalline cellulose, and uniformly mixing mixture; leading in shielding gas, and performing anti-oxidation decomposition treatment; performing stirring and heating to obtain microcrystalline cellulose solution; dripping the solution into water by a falling-ball method to obtain cellulose hydrogel; washing the cellulose hydrogel by a gradient washing method; performing vacuum freezedrying to obtain porous cellulose-based foaming buffer packaging materials; performing filtration, purification and rotary evaporation on the residual solvent, and recycling ionic liquid. The prepared cellulose packaging materials can be biodegraded and are environmentally friendly, a preparation process of the cellulose-based foaming buffer packaging materials is simplified, chemical additivesare omitted, and the packaging materials has the advantages that the materials are environmentally friendly and free from pollution.

The invention discloses a method for improving the antioxidant activity of pueraria flavone. The method comprises the steps of dispersing pueraria flavone into a buffer solution; adding beta-glucosidase for reaction at 30-50 DEG C; and filtering after the reaction is completed, extracting filtrate by using ethyl acetate, and carrying out reduced pressure distillation and drying on an extract solution. The pueraria flavone powder is subjected to hydrolytic treatment by adopting flavor hydrolase beta-glucosidase, and pueraria flavonoid glycoside is hydrolyzed into aglycone with higher antioxidant activity and better lipid solubility to expand the application of the pueraria flavone powder in the industries of foods and the like.

The invention relates to a Cu-BTC MOF carbonized porous material as well as a preparation method and application thereof. The preparation method of the Cu-BTC MOF carbonized porous material comprises the following steps of calcining and carbonizing Cu-BTC MOF, wherein the calcining and carbonizing temperature is 400-800 DEG C, the calcining and carbonizing temperature is raised to a specified temperature at the speed of 1-3 DEG C per minute, and then the calcining and carbonizing temperature is kept for 3 hours, and removing copper ions by using concentrated hydrochloric acid, washing with water until the material is neutral, and drying at 100 DEG C to obtain the material. After the Cu-BTC MOF material is carbonized, the affinity of the material to aflatoxin B1 can be obviously enhanced. The Cu-BTC MOF carbonized porous material is used for adsorbing aflatoxin in vegetable oil and has the advantages of being easy to operate, large in adsorption capacity, high in adsorption speed and the like; the method has the advantages of simple operation, low pollution, high safety, almost no influence on nutritional indexes of the vegetable oil after adsorption, no cytotoxicity, and certain application prospect.

The invention discloses a method for synthesizing nicotine salt by using a micro-channel reactor under a solvent-free condition. The method comprises the following steps: respectively adding liquid nicotine and acetic acid at a molar ratio of 1: (1.1-6) into an injector, pumping the liquid nicotine and acetic acid into the micro-channel reactor, heating to 25-95 DEG C to react for 85-706 seconds,and discharging through a liquid receiving port after the pressure of the system reaches a certain value, thereby obtaining nicotine salt. The method has the advantages of mild reaction conditions, simple operation, low raw material cost, no solvent addition, environmental protection, no post-treatment purification and high yield, and the prepared nicotine salt has the advantages of stable state,high purity, effective reduction of the irritation of nicotine, and suitableness for industrial production.

The invention discloses sweet potato powder capable of maintaining beauty and slimming a body and a making method thereof. The making method comprises the following steps that 1, sweet potatoes are cleaned and peeled and then cut into slices and blocks with the particle size of 1-15 mm to obtain sweet potato strips, the sweet potato strips are soaked in color protecting liquid to be subjected to color protecting for 10 min to 40 min, filtering is conducted, and a color protecting product is obtained; 2, the color protecting product is put into enzyme liquid at the temperature of 30 DEG C to 40 DEG C to be subjected to enzymolysis for 1 h to 4 h, filtering is conducted, and an enzymolysis product is obtained; 3, the enzymolysis product is dried for 5 h to 15 h at the temperature of 50 DEG C to 80 DEG C and then crushed to be 100 mesh to 500 mesh. The sweet potato powder is made by carefully selecting the fresh sweet potatoes as raw materials through the steps of cleaning, cutting to strips, color protecting, enzymolysis conducting, drying and crushing. Compared with the prior art, the sweet potato powder has the advantages that rapidness and high efficiency are achieved, no foreign matter is added, and bioactive components can be effectively protected and is more beneficial for human body absorption and high in nutritional health-care value.

The invention belongs to the field of electrocatalysts, in particular to a strontium-doped noble metal oxide oxygen evolution electrocatalyst and a preparation method thereof. The invention utilizes the change of the solubility of strontium hydroxide with temperature, reacts the high-temperature strontium hydroxide solution with metal chloric acid, and then cools down the outer layer of the noble metal hydroxide to precipitate strontium hydroxide, and calcines the precipitate at high temperature to obtain strontium-doped The oxygen evolution electrocatalyst of miscellaneous noble metal oxides; the method of the invention is simple, has low equipment requirements, and the obtained oxygen evolution electrocatalyst has good stability, many reaction sites and high catalytic activity.

The invention discloses health care non-transgenic sweet potato powder and a preparation method thereof. The preparation method comprises the following steps of (1) cleanly cleaning sweet potatoes by clean water; draining the water; crushing the sweet potatoes to 2 to 20 meshes; then, freeze drying is performed at -15 DEG C to -35 DEG C for 5 to 15 hours; obtaining drying powder; (2) adding the drying powder into an ultrafine grinder to be subjected to ultrafine grinding into 1 to 10 mum; obtaining ultrafine powder; (3) adding the ultrafine powder into enzyme liquid with the temperature being 35 to 55 DEG C; performing stirring for 3 to 6 hours at the rotating speed of 300 to 800 r / min; then, performing centrifugal separation; obtaining wall-broken powder; (4) performing vacuum drying on the wall-broken powder at 45 to 75 DEG C for 5 to 15 hours; then, performing ultraviolet sterilization. The non-transgenic sweet potatoes are used as raw materials; crushing, freeze drying, superfine grinding and wall breaking are performed to obtain the health care non-transgenic sweet potato powder. Compared with the prior art, the preparation method provided by the invention has the advantages that in the wall breaking process, the wall breaking goal is achieved through enzymolysis; the speed and the efficiency are high; no foreign material is added; bioactive substances can be effectively protected; the human body adsorption is facilitated; the nutrition and health care values are high.

The invention discloses a preparation method of resin carbon for blood purification. The preparation method comprises the following steps: preparing chloromethylated styrene-divinylbenzene microspheres from p-chloromethyl styrene monomers and divinylbenzene monomers; preparing polystyrene-divinylbenzene microspheres from the chloromethylated styrene-divinylbenzene microspheres; dipping the polystyrene-divinylbenzene microspheres by using a zinc-chloride solution to obtain dipped microspheres; and carbonizing the dipped microspheres, and activating the dipped microspheres after the carbonizing so as to obtain a finished product. The preparation method disclosed by the invention has the obvious effects that the resin carbon with the excellent absorbing effect for middle molecules can be prepared and obtained at relatively low temperature and within shorter time, the preparation process is simple, the pollution is slight, the product yield is high and the purity is high.

The invention relates to a Cu-BTC MOF carbonized porous material and its preparation method and application. The preparation method of Cu-BTC MOF carbonized porous material includes calcining and carbonizing Cu-BTC MOF; the temperature of the calcining and carbonization is 400-800°C, and the temperature of the calcining and carbonization is raised to a specified temperature at 1-3°C per minute, and then kept for 3h ; Then use concentrated hydrochloric acid to remove copper ions, wash with water until the material is neutral, and dry at 100°C. In the present invention, the Cu-BTC MOF material is carbonized, which can significantly enhance the resistance of the material to aflatoxin B. 1 affinity. Using the Cu-BTC MOF carbonized porous material of the present invention to adsorb aflatoxin in vegetable oil has the advantages of simple operation, large adsorption capacity, fast adsorption speed, etc.; small pollution, high safety, and almost no damage to the nutritional index of vegetable oil after adsorption. impact and no cytotoxicity, has certain application prospects.

The invention belongs to the field of biomedical materials and tissue engineering, and in particular relates to a bionic skin dressing having a 3D micropattern structure and a preparation method thereof. The biomimetic skin dressing is obtained by curing a polymer composite gel into a composite gel film having a 3D micropattern structure, and then using calcium alginate for high hydrophilic surface treatment. The polymer composite gel is prepared by compounding RGD-peptide-containing methacrylate gelatin and methacrylate hyaluronic acid in a mass ratio of 1:3 to 3:1. The bionic skin dressing having the 3D micropattern structure has a straight stripe groove or a mesh stripe groove array, RGD peptide in the groove array can promote cell adhesion, and the calcium alginate in a convex array structure region can effectively inhibit cell growth, so that cells can selectively grow on the surface of the bionic skin dressing to form the cell micropattern, and the 3D micropattern structure can promote the ability of stem cells to differentiate into fibroblasts and angioblast in vivo and in vitro.

The invention relates to a method for manufacturing a decorative Tibetan tea brick, which is prepared by collecting tea leaves, testing, fixing, fermenting, drying, watering and stirring, pressing into a mold, drying again, preparing brackets, and packaging. The invention has a low-temperature natural manufacturing process, no chemical additives, and can keep the beneficial substances in the tea leaves. The prepared tea bricks are not only convenient for storage, carrying and drinking, but also have the functions of emitting tea fragrance indoors and improving indoor air and decoration environment.