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Haematococcus pluvialis cell wall breaking method

A technology of Haematococcus pluvialis and cells, which is applied in the field of cell wall breaking of Haematococcus pluvialls, which can solve the problems of affecting yield, difficult crushing, and affecting the extraction rate of astaxanthin, and achieves rapid and efficient wall breaking without foreign matter The effect of adding and effectively protecting biologically active ingredients

Active Publication Date: 2017-07-18
YUNNAN AIERKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the advancement of culture technology, the content of astaxanthin has increased significantly, breaking through 5%, and even reaching more than 6%. The theoretical output of astaxanthin has doubled, but the higher the content of astaxanthin, the more the In order to better protect themselves, the cell cellulose increases, the thicker and tougher the cell wall has reached 6-8 microns, and it is more difficult to break. The rate of cell breakage directly affects the extraction rate of astaxanthin, directly affecting the actual output. If the general method of breaking the wall is used, such as freeze-thaw method, ultrasonic method, biological enzymatic hydrolysis method, high-pressure homogenizer, colloid mill, etc., one or more confluences are used to break the wall, and the breaking rate can only reach 80-85%. , so the wall-breaking technology has seriously restricted the large-scale development of Haematococcus pluvialis cultivation and the expansion of the subsequent astaxanthin industry chain. Now it has become the most breakthrough core issue of Haematococcus pluvialis development technology

Method used

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  • Haematococcus pluvialis cell wall breaking method
  • Haematococcus pluvialis cell wall breaking method
  • Haematococcus pluvialis cell wall breaking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The method for breaking the cell wall of Haematococcus pluvialis comprises the following steps:

[0038] Step (1), take 50kg of Haematococcus pluvialis wet algae mud after pressure filtration, and its solid content is 60%, so as to obtain 30kg of solid content, put it in the freezer and freeze at -25°C for 12 hours, and measure the wet algae The temperature of the mud is -18°C, crushed into powder;

[0039] Take 45kg of edible alcohol and 55kg of water to make 100kg of alcohol solution with a mass concentration of 45%, put it in the freezer, freeze at -35°C for 12 hours, and measure the alcohol solution to be -25°C; then wet the algae mud at -18°C , scattered among them;

[0040] Step (2), adding food-grade antioxidant vitamin E to the alcohol solution in step (1), the addition amount is 90g, to obtain a feed liquid;

[0041] In step (3), the material liquid obtained in step (2) is ground and mixed evenly by a colloidal mill, and then the wall is broken once with a hi...

Embodiment 2

[0044] The method for breaking the cell wall of Haematococcus pluvialis comprises the following steps:

[0045] Step (1), take 32kg of edible alcohol and 48kg of water to make 80kg of alcohol solution with a mass concentration of 40%, put it in the freezer, freeze at -35°C for 10 hours, and measure the alcohol solution to be -25°C; then take dry rain Haematococcus powder algal powder 20kg is dispersed in it;

[0046] Step (2), adding food-grade antioxidant phospholipids to the alcohol solution in step (1) in an amount of 20 g to obtain a feed liquid;

[0047] In step (3), the material liquid obtained in step (2) is ground and mixed evenly by a colloidal mill, and then the wall is broken once with a high-pressure homogeneous nanomachine at a pressure of 120-130mpa to obtain Haematococcus pluvialis cells Wall-breaking liquid material; the wall-breaking process is carried out under the conditions of cold chain low temperature and light-proof sealing.

[0048] Through microscope...

Embodiment 3

[0050] The method for breaking the cell wall of Haematococcus pluvialis comprises the following steps:

[0051]Step (1), take 24kg of edible alcohol and 56kg of water to make 80kg of alcohol solution with a mass concentration of 30%, put it in the freezer, freeze it at -35°C for 18 hours, and measure the alcohol solution to be -30°C; then take the dry 25kg of Haematococcus pluvialis flake algae powder are dispersed in it;

[0052] Step (2), adding food-grade antioxidant butylated hydroxyanisole to the alcohol solution in step (1) in an amount of 60 g to obtain a feed liquid;

[0053] In step (3), the material liquid obtained in step (2) is ground and mixed evenly by a colloidal mill, and then the wall is broken once with a high-pressure homogeneous nanomachine at a pressure of 120-130mpa to obtain Haematococcus pluvialis cells Wall-breaking liquid material; the wall-breaking process is carried out under the conditions of cold chain low temperature and light-proof sealing.

...

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Abstract

The invention belongs to the technical field of microalgae microorganisms and relates to a wall-breaking method for haematococcus pluvialis cells which are rich in a biological active substance: astaxanthin. The wall-breaking method for the haematococcus pluvialis cells provided by the invention comprises the following three steps: 1, freezing and ice-crystallizing the haematococcus pluvialis cells and dispersing the cells in a flowing liquid below 0 DEG C; 2, adding a bioactive component which protects the haematococcus pluvialis into the liquid before the ice-crystallized algae cells are broken so as to reduce a protectant which is oxidized and decomposed after wall breaking; and 3, after uniformly grinding by a colloid mill, carrying out ultra-high-pressure wall-breaking by using an ultra-high-pressure homogeneous nanometer machine. The wall-breaking process provided by the invention is carried out under a condition of low-temperature, cold chain and close away from light and breaks walls by means of the characteristic of nanometer crushing by using the ultra-high-pressure homogeneous nanometer machine and has the advantages of rapidness, efficiency and no addition of foreign matters and can be used for effectively protecting the bioactive component.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to the technology for breaking the cell walls of Haematococcus pluvialis cells rich in astaxanthin bioactive substances. Background technique [0002] Haematococcus pluvialis is a single-celled green algae that lives in fresh water. Under certain conditions, it can accumulate a large amount of carotenoids, of which more than 80% are astaxanthin and esters. Astaxanthin has important physiological and biological functions such as anti-oxidation, anti-tumor and increasing immunity. [0003] Haematococcus pluvialis cells have cellulosic cell walls, the cell wall thickness is generally 3-5 microns, and the texture is tough. With the advancement of culture technology, the content of astaxanthin has increased significantly, breaking through 5%, and even reaching more than 6%. The theoretical output of astaxanthin has doubled, but the higher the content of astaxanthin, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06C12R1/89
CPCC12N1/066
Inventor 张勇王天黎王琦吴秋瑾梁文伟
Owner YUNNAN AIERKANG BIOTECH
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