Method for extracting astaxanthin from haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, applied in the field of extracting astaxanthin

Inactive Publication Date: 2014-06-25
QINGDAO ZHONGREN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the above-mentioned problems existing in the prior art, the object of the present invention is to provide a method for extracting astaxanthin from Haematococcus pluvialis, to solve Commercially available astaxanthin is chemically synthesized and expensive

Method used

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Embodiment

[0015] The method for extracting astaxanthin from Haematococcus pluvialis of the present embodiment comprises the following specific steps:

[0016] (1) Sporophyte collection by breaking the wall: take fresh wet algae mud, add 2 times the mass ratio of 3mol / L HCl, stir evenly, stir for 3 minutes at 50°C, and stir at a speed of 180 times per minute, then cool rapidly, and take after centrifugal washing Algae;

[0017] (2) Extract astaxanthin: add 2 times ethanol to the broken sporophytes, shake at 10°C for 5 minutes, centrifuge, recover ethanol, repeat twice, combine the second ethanol, concentrate in vacuum at low temperature, recover ethanol by volatilization, and leave The substance is astaxanthin.

[0018] Because the spore wall of Haematococcus pluvialis is thick and solid, and the sporophyte is very small, it brings many difficulties to breaking the wall. The present invention adopts the acid-heat method, takes fresh wet algae mud, and adds 2 times of HCl with a mass rat...

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Abstract

The invention discloses a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of (1) breaking walls of sporophore: taking fresh wet algae mud, adding 3 mol / L HCl with an amount of two times by mass of the fresh wet algae mud, stirring uniformly, stirring for 3 min at a temperature of 50 DEG C with a stirring speed of 180 times per minute, cooling rapidly, centrifuging and washing and taking the algae bodies; and (2) extracting astaxanthin: adding ethanol into the wall-broken sporophore with an amount of two times of the sporophore, oscillating for 5 min at a low temperature of 10 DEG C, centrifuging, recovering ethanol, repeating for 2 times, merging the recovered ethanol; concentrating at a low temperature and in vacuum; and recovering ethanol by evaporation, and thus the left material being astaxanthin. The method is simple and can be easily understood, is reasonable in operation, enables the haematococcus pluvialis to perform nutritive growth relatively well, is relatively fast in transformation from nutritive growth to non-nutritive growth, and accumulates relatively much haematococcus pluvialis, thereby increasing efficiency and stability of culture, transformation and accumulation and effectively preventing contamination of miscellaneous algae during a culture process. The manufactured astaxanthin is relatively low in cost.

Description

[0001] technical field [0002] The invention belongs to, and specifically relates to a method for extracting astaxanthin from Haematococcus pluvialis. [0003] Background technique [0004] Astaxanthin is a type of carotenoid that is mainly used as a natural colorant for fish. It has super anti-oxidation ability, protects the skin, improves immunity, inhibits cancer and other functions, and is widely used in aquatic products, cosmetics, medicine and other industries. Haematococcus pluvialis belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococcus pluvialis, and Haematococcus pluvialis. Haematococcus pluvialis is currently known as the organism with the highest astaxanthin content. It accumulates astaxanthin in stressful environments, such as nutrient deficiency, strong light, etc., and its astaxanthin content can reach up to 1-4% of the cell mass , is an algae with great potential for large-scale production of astaxanthin. [0005] Haematococcus pluvialis is a singl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 张述智夏伟朱绍辉张浩王晓丽徐权汗李之详许团辉
Owner QINGDAO ZHONGREN PHARMA
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