Bacillus megaterium nbnc-104 for controlling root-knot nematode, its compound microbial agent and application

By using Bacillus megaterium NBNC-104 and its compound bacterial agents, the problems of resistance to chemical control of nematodes and the lack of biological control resources have been solved, achieving efficient and stable control of root-knot nematodes, which is suitable for organic agriculture.

CN120060013BActive Publication Date: 2025-11-21HUBEI BIOPESTICIDE ENG RES CENT
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Patent Information

Application Number
CN202510182339.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-02-19
Publication Date
2025-11-21
Estimated Expiration
2045-02-19

AI Technical Summary

Technical Problem

Existing chemical control methods for nematodes are prone to inducing resistance and pose food and environmental safety risks. Biological control resources are limited and difficult to effectively control root-knot nematodes.

Method used

Nematicides were prepared by fermentation broth using Bacillus megaterium NBNC-104 and its compound bacterial agents, including Bacillus thuringiensis NBIN-863 and Stizemonas kunmingensis NBNH-006, and the synergistic effect was used to improve the control effect.

Benefits of technology

It significantly improves the control effect against southern root-knot nematodes. The fermentation liquid has a control effect of up to 89.25% against root-knot nematodes, which is comparable to chemical agents. Moreover, it can be stably established in the soil and is suitable for organic agricultural production.

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Abstract

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.
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