Microbe sample preservation solution for gene detection, preparation method and kit thereof, and application of kit

A gene detection and microbial technology, applied in the field of bioengineering, can solve the problems of reduced inactivation effect, cell rupture, easy to be degraded and damaged, etc., and achieve the effect of comprehensive microbial inactivation ability, maintenance of cell structure, and thorough inactivation effect
CN108546646AActive Publication Date: 2018-09-18知几未来(成都)生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
知几未来(成都)生物科技有限公司
Publication Date
2018-09-18

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Abstract

The invention provides a microbe sample preservation solution for gene detection, a preparation method and a kit thereof, and an application of the kit, and belongs to the technical field of bioengineering. The microbe sample preservation solution for gene detection has effective and comprehensive microbe inactivation capability; and low-concentration ethanol is used to thoroughly inactivate bacteria in the storage process of a sample, and avoids inactivation failure caused by the stress reaction of bacteria due to high-concentration ethanol. The microbe sample preservation solution has good permeability on the premise of not destroying the cell structure, and effectively destroys the protein activity of bacteria and viruses and the activities of various nucleases to make the bacteria, viruses and nucleases not have of infection or proliferation ability, so the thorough inactivation effect is achieved. A glycerol-containing cytoprotective agent and a physiological concentration buffersolution are used to keep the cell structure of microbes and prevent the cell structure from be ruptured in order to protect the integrity of nucleic acids in cells; and the microbe sample preservation solution contains no high-toxicity components or volatile components, so no volatile poisoning occurs during use.
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Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a microbial sample preservation solution for gene detection, a preparation method, a kit and an application thereof. Background technique

[0002] At present, the oral / fecal bacterial preservation solutions on the market are mainly composed of ethanol / methanol / SDS, and are prepared into weakly alkaline isotonic solutions, which can kill the bacteria in the sample, but will cause damage to the cell structure, resulting in Cell contents are released and cannot be detected by immunological or other methods. At the same time, methanol\guanidine isothiocyanate in the preservation solution is volatile or highly toxic, and it is easy to cause volatilization during use and cause harm to operators.

[0003] The existing preservation solution is inactivated with high-purity ethanol; some bacteria will have a stress response to high-concentration ethanol, resulting in a reduced inac...

Claims

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