Vagina microbial immunofluorescence staining solution
An immunofluorescent staining and microbiological technology, which is applied in the field of vaginal microbial immunofluorescent staining solution, can solve the problems of large data difference, poor stability effect, short storage time of fluorescent staining solution, etc., and achieve efficiency improvement, fluorescence retention time increase, Improve the effect of dyeing effect
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Embodiment 1
[0030] A vaginal microorganism immunofluorescent staining liquid, comprising the following raw materials in parts by weight: 5 parts of fluorescent dye, 2 parts of auxiliary dye, 1 part of stabilizer, 1 part of penetrant, 2 parts of bacteriostatic agent, and anti-quenching agent 1 part, 2 parts of permeabilization agent, 2 parts of buffer solution, 5 parts of cell protection agent and 20 parts of purified water. The cell protection agent is one of oil, dimethyl sulfoxide, propylene glycol, and ethylene glycol.
[0031] The fluorescent staining agent is one of acridine orange, fluorescein isothiocyanate, tetraethylrhodamine, propidium iodide, pyrolorubin G, phycoerythrin, methyl green or brilliant green.
[0032] The stabilizer is one of glycerol, propylene glycol and sorbitol.
[0033] The penetrating agent is one of dimethylsulfoxide, dimethylacetamide and N-N-dimethylformamide.
[0034] The auxiliary dye is one of 6-carboxyfluorescein, 5-carboxyfluorescein succinimide ester...
Embodiment 2
[0052] A vaginal microbiological immunofluorescence staining solution, comprising the following raw materials in parts by weight: 6 parts of fluorescent dye, 2 parts of auxiliary dye, 2 parts of stabilizer, 2 parts of penetrant, 3 parts of bacteriostatic agent, and anti-quenching agent 2 parts, 3 parts of permeabilization agent, 3 parts of buffer solution, 51 parts of cell protection agent and 30 parts of purified water. The cell protection agent is one of oil, dimethyl sulfoxide, propylene glycol, and ethylene glycol.
[0053] The fluorescent staining agent is one of acridine orange, fluorescein isothiocyanate, tetraethylrhodamine, propidium iodide, pyrolorubin G, phycoerythrin, methyl green or bright green.
[0054]The stabilizer is one of glycerol, propylene glycol and sorbitol.
[0055] The penetrating agent is one of dimethylsulfoxide, dimethylacetamide and N-N-dimethylformamide.
[0056] The auxiliary dye is one of 6-carboxyfluorescein, 5-carboxyfluorescein succinimide ...
Embodiment 3
[0074] A vaginal microorganism immunofluorescence staining solution, comprising the following raw materials in parts by weight: 8 parts of fluorescent dye, 3 parts of auxiliary dye, 3 parts of stabilizer, 3 parts of penetrant, 5 parts of bacteriostatic agent, and anti-quenching agent 2 parts, 4 parts of permeabilization agent, 4 parts of buffer solution, 6 parts of cell protection agent and 40 parts of purified water. The cell protection agent is one of oil, dimethyl sulfoxide, propylene glycol, and ethylene glycol.
[0075] The fluorescent staining agent is one of acridine orange, fluorescein isothiocyanate, tetraethylrhodamine, propidium iodide, pyrolorubin G, phycoerythrin, methyl green or bright green.
[0076] The stabilizer is one of glycerol, propylene glycol and sorbitol.
[0077] The penetrating agent is one of dimethylsulfoxide, dimethylacetamide and N-N-dimethylformamide.
[0078] The auxiliary dye is one of 6-carboxyfluorescein, 5-carboxyfluorescein succinimide es...
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