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Vagina microbial immunofluorescence staining solution

An immunofluorescent staining and microbiological technology, which is applied in the field of vaginal microbial immunofluorescent staining solution, can solve the problems of large data difference, poor stability effect, short storage time of fluorescent staining solution, etc., and achieve efficiency improvement, fluorescence retention time increase, Improve the effect of dyeing effect

Pending Publication Date: 2022-03-18
济南德亨医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a vaginal microbiological immunofluorescent staining solution, which has the advantages of stable dyeing effect, long storage time and high dyeing rate, and solves the problem of the dyeing stability of the existing fluorescent staining solution in use. The effect is not good, which leads to the phenomenon of large data difference in the detection, and at the same time solves the problem of short storage time of the existing fluorescent staining solution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A vaginal microorganism immunofluorescent staining liquid, comprising the following raw materials in parts by weight: 5 parts of fluorescent dye, 2 parts of auxiliary dye, 1 part of stabilizer, 1 part of penetrant, 2 parts of bacteriostatic agent, and anti-quenching agent 1 part, 2 parts of permeabilization agent, 2 parts of buffer solution, 5 parts of cell protection agent and 20 parts of purified water. The cell protection agent is one of oil, dimethyl sulfoxide, propylene glycol, and ethylene glycol.

[0031] The fluorescent staining agent is one of acridine orange, fluorescein isothiocyanate, tetraethylrhodamine, propidium iodide, pyrolorubin G, phycoerythrin, methyl green or brilliant green.

[0032] The stabilizer is one of glycerol, propylene glycol and sorbitol.

[0033] The penetrating agent is one of dimethylsulfoxide, dimethylacetamide and N-N-dimethylformamide.

[0034] The auxiliary dye is one of 6-carboxyfluorescein, 5-carboxyfluorescein succinimide ester...

Embodiment 2

[0052] A vaginal microbiological immunofluorescence staining solution, comprising the following raw materials in parts by weight: 6 parts of fluorescent dye, 2 parts of auxiliary dye, 2 parts of stabilizer, 2 parts of penetrant, 3 parts of bacteriostatic agent, and anti-quenching agent 2 parts, 3 parts of permeabilization agent, 3 parts of buffer solution, 51 parts of cell protection agent and 30 parts of purified water. The cell protection agent is one of oil, dimethyl sulfoxide, propylene glycol, and ethylene glycol.

[0053] The fluorescent staining agent is one of acridine orange, fluorescein isothiocyanate, tetraethylrhodamine, propidium iodide, pyrolorubin G, phycoerythrin, methyl green or bright green.

[0054]The stabilizer is one of glycerol, propylene glycol and sorbitol.

[0055] The penetrating agent is one of dimethylsulfoxide, dimethylacetamide and N-N-dimethylformamide.

[0056] The auxiliary dye is one of 6-carboxyfluorescein, 5-carboxyfluorescein succinimide ...

Embodiment 3

[0074] A vaginal microorganism immunofluorescence staining solution, comprising the following raw materials in parts by weight: 8 parts of fluorescent dye, 3 parts of auxiliary dye, 3 parts of stabilizer, 3 parts of penetrant, 5 parts of bacteriostatic agent, and anti-quenching agent 2 parts, 4 parts of permeabilization agent, 4 parts of buffer solution, 6 parts of cell protection agent and 40 parts of purified water. The cell protection agent is one of oil, dimethyl sulfoxide, propylene glycol, and ethylene glycol.

[0075] The fluorescent staining agent is one of acridine orange, fluorescein isothiocyanate, tetraethylrhodamine, propidium iodide, pyrolorubin G, phycoerythrin, methyl green or bright green.

[0076] The stabilizer is one of glycerol, propylene glycol and sorbitol.

[0077] The penetrating agent is one of dimethylsulfoxide, dimethylacetamide and N-N-dimethylformamide.

[0078] The auxiliary dye is one of 6-carboxyfluorescein, 5-carboxyfluorescein succinimide es...

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Abstract

The invention relates to the technical field of medical detection, and discloses a vagina microorganism immunofluorescence staining solution. Comprising the following raw materials in parts by weight: 5-8 parts of a fluorescent coloring agent, 2-3 parts of an auxiliary dye, 1-3 parts of a stabilizer, 1-3 parts of a penetrant, 2-5 parts of a bacteriostatic agent, 1-2 parts of an anti-quenching agent, 2-4 parts of a permeable agent, 2-4 parts of a buffer solution, 5-6 parts of a cell protective agent and 20-40 parts of purified water. According to the vagina microbial immunofluorescence staining solution, the stability of the fluorescent staining solution during staining can be improved by adding the stabilizer, the fluorescence retention time is prolonged, the problem that the staining fluorescence is not obvious due to poor staining stability and the detection data is influenced is avoided, and meanwhile, the antibacterial activity of the fluorescent staining solution is improved by adding the bacteriostatic agent; the fluorescent staining solution can be stored for a longer time, the permeability agent and the penetrant added in the fluorescent staining solution can improve the permeability to cell membranes, staining of cells by fluorescein can be accelerated, and the practicability of the vagina microbial immunofluorescence staining solution is enhanced.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a vaginal microorganism immunofluorescence staining solution. Background technique [0002] There are many microbial flora symbiotic in the female lower reproductive tract, which have a natural defense function against the invasion of pathogens. However, when the vaginal microecology is out of balance and the natural defense function is destroyed, pathogens are easy to invade, leading to vaginitis, cervicitis, pelvic inflammatory disease, etc. The occurrence of diseases increases the risk of AIDS and cervical cancer; women with vaginitis will affect the quality of sperm during pregnancy, and even lead to infertility; even during pregnancy, it is easy to cause intrauterine infection, birth canal infection and other links to infect the fetus , newborns, resulting in miscarriage, premature birth, congenital deformities, mental retardation and other serious consequences. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N21/64G01N33/569G01N33/58
CPCG01N1/30G01N21/6486G01N33/569G01N33/582G01N2001/302
Inventor 亓德胜谭四军
Owner 济南德亨医学科技有限公司
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