The invention provides a preparation method of a right-handed lansoprazole crystal form. The preparation method is carried out in an ethyl acetate-isopropyl ether system. The preparation method comprises the following steps: (1) dissolving a purified lansoprazole hydrate in five times of mass/volume ratio of ethyl acetate, adding an appropriate amount of saturated sodium bicarbonate solution, stirring for 10 min, carrying phase separation, adding an appropriate amount of anhydrous sodium sulfate and 0.2g of active carbon into an organic phase, stirring for 30 min, filtering, and washing with ethyl acetate; (2) merging filtrate and washing liquor, slowly adding isopropyl ether two times of the volume of the ethyl acetate, adding a crystal seed A, and stirring for 10 min; (3) cooling to 0 to 10 DEG C, and precipitating solids; (4) performing suction filtration, and washing a filter cake with isopropyl ether; and (5) drying in vacuum for 2h at 40 DEG C, thus obtaining white crystals with relatively large density. By adopting the preparation method, the defect in the original patent that a great amount of solvent is consumed by using a methylene dichloride-isopropyl ether system can be overcome, an obtained crystal form is high in melt point, large in bulk density and high in stability, and the preparation method is small in environmental pollution during the preparation process, simple in operation, high in production efficiency and suitable for mass production.