Method for extracting desoxyribonucleic acid from formalin fixed and paraffin embedded tissues

Abstract

The invention relates to a method for extracting desoxyribonucleic acid from formalin fixed and paraffin embedded tissues, and belongs to the technical field of nucleic acid application in biology. The method comprises the following steps of: preparing special lysis solution, adding the lysis solution into paraffin section tissues, boiling at high temperature for 30 minutes, and centrifuging to take supernate; adding absolute ethanol for uniform mixing, and adding the mixed solution into a silicon membrane absorption column for centrifuging; rinsing by using protein-free liquid and salt-free rinsing liquid; and eluting by using elution buffer. In the method, a toxic reagent of dimethylbenzene is not used for dewaxing, and harm to bodies of experimenters is avoided; and precious protease Kis not needed to perform long-time incubation enzymolysis, the operation is simple and quick, the extracted desoxyribonucleic acid in genome is high in quality and stability, and the cost and time can be saved to the greatest degree. The extracted product is subjected to polymerase chain reaction (PCR) detection, long segments with about 750pb can be obtained through amplification, and the work in the aspects such as scientific research, biomedicine and the like is greatly facilitated.

Description

technical field [0001] The invention relates to a method for extracting deoxyribonucleic acid from formalin-fixed paraffin-embedded tissues, belonging to the technical field of nucleic acid applications in organisms. Background technique [0002] Formalin is not only a preservative, but also has a good effect on maintaining the shape of many biological specimens. It is superior to other fixatives in terms of maintaining the integrity of tissue cell structure, antigen measurability, and tissue permeability. Therefore, it has been widely used in the research of biological evolution, phylogeny, endangered species protection, population genetics and other disciplines, as well as in clinical testing and medical science research. Therefore, a relatively simple, effective, safe and feasible method for extracting high-quality genomic deoxyribose nucleic acid (Deoxyribonucleic acid, hereinafter referred to as DNA) from formalin fixed and paraffin embedded tissues (FFPE, hereinafter ...

AI Technical Summary

Problems solved by technology

[0003] At present, the traditional paraffin-embedded tissue DNA extraction mostly adopts the method of xylene dewaxing and proteinase K incubation. The whole extraction process of this method is long, and proteinase K is expensive, and the operation is very cumbersome. Time-consuming and reagents are required, and the amount of DNA obtained is relatively low. few

And prone to DNA chain degradation into small fragments of DNA

It is not suitable for processing a large number of specimens, and the organic solvent xylene reagent used in the extraction process is likely to cause environmental pollution and harm to the operator's body

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