Method for manufacturing paraffin-embedded tissue cell specimen

A technology of tissue cells and paraffin embedding, applied in the preparation of test samples, etc., can solve the problems of toxicity and other problems, achieve the effect of fewer steps, wide production range, and improved accuracy

Inactive Publication Date: 2014-07-23
QINGDAO MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional production time takes 3-5 days, and the immunohistochemical time usually takes 5-7 days to get the diagnosis report
And in the production process, due to the volatilization of formaldehyde and xylene, it will cause certain toxicity to the human body.
Therefore, the current production method needs to be improved urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1: A kind of preparation method of paraffin embedding of pleural effusion or peritoneal effusion cell specimen of the present invention, concrete steps are as follows:

[0065] (1) Precipitate cells: put 100ml of extracted pleural effusion or peritoneal effusion into a clean vessel and let it rest for 1 hour, so that the cells in pleural effusion or peritoneal effusion naturally settle to the bottom of the vessel;

[0066] (2) Collect tissue cells: Discard the supernatant, put about 50ml of the bottom sediment into 10 test tubes with a volume of 5ml, and centrifuge at 2000 rpm for 10 minutes, and discard the supernatant. Go, finally concentrate the tissue cells in the 10 test tubes into one test tube until more tissue cell numbers are obtained;

[0067] (3) Coagulate tissue cells: add 10% neutral buffered formaldehyde to the final precipitated tissue cells, break up the precipitated cells with a pipette, centrifuge for 3 minutes, and take out the semi-coagula...

Embodiment 2

[0114] Embodiment 2: A method for making paraffin-embedded cell samples of pleural effusion or peritoneal effusion according to the present invention, the specific steps are as follows:

[0115] (1) Precipitate cells: put 500ml of extracted pleural effusion or peritoneal effusion into a clean vessel and let it rest for 2 hours, so that a large amount of cells in pleural effusion or peritoneal effusion naturally settle to the bottom of the vessel;

[0116] (2) Collect tissue cells: Discard the supernatant, put 100ml of the bottom sediment into 10 test tubes with a volume of 10ml and centrifuge at 2500 rpm for 5 minutes, then discard the supernatant , finally concentrating the tissue cells in the 10 test tubes into one test tube until a larger number of tissue cells is obtained;

[0117] (3) Coagulate tissue cells: add 10% neutral buffered formaldehyde to the final precipitated tissue cells, break up the precipitated cells with a pipette, and centrifuge for 3 minutes, let them s...

Embodiment 3

[0163] Embodiment 3: A kind of preparation method of paraffin embedding of urine, lymph fluid or cell suspension cell specimen of the present invention, concrete steps are as follows:

[0164] (1) Collect tissue cells by centrifugation: First, use a large amount of urine, lymph or cell suspension in multiple 50ml centrifuge tubes, centrifuge on a large centrifuge, discard the supernatant, and finally concentrate the sediment Centrifuge again in a 5ml test tube, discard the supernatant; because the total amount of lymph fluid and cell suspension is small, but the cell content is large, it can be directly put into the test tube for centrifugation and discard the supernatant;

[0165] (2) The embedding includes primary embedding and secondary embedding. The primary embedding is to add a substance with a melting point of 45°C to 52°C into a tissue cell in the urine, lymph or cell suspension. 5% agar, quickly condensed into a ball at room temperature, put into gauze and wrap it ...

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PUM

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Abstract

The invention provides a method for manufacturing a paraffin-embedded tissue cell specimen. The method is characterized by comprising the following steps: precipitating and performing centrifugal treatment on a tissue cell specimen, subsequently extracting the tissue cell specimen, adding an environmental-friendly biologic synthesis agent, synchronously fixing, dehydrating, transparentizing and performing paraffin dipping treatment on the tissue cell specimen by an ultrasonic tissue treatment instrument or a medical microwave oven, slicing the paraffin-embedded tissue, and dyeing and sealing the paraffin-embedded tissue slice. The method has the advantages that the operation is rapid, no pollution is caused, the operation is simple and convenient, a dyeing process can be performed together with daily work, a molecular pathological examination can be also performed, and the result is stable. The positive detection rate and the accuracy in pathological diagnosis are improved.

Description

technical field [0001] The invention belongs to the detection method of cytology in the field of clinical pathological diagnosis and scientific research, in particular to a preparation method of tissue cell specimen paraffin embedding. Background technique [0002] Detecting tumor cells through pleural, ascites and lymph fluid is one of the commonly used detection methods for clinical pathological diagnosis. At present, more methods are used, such as smear method, TCT, LCT, etc. The cell smears produced by them are evenly distributed, clear in shape, and positive. The diagnostic rate is high, but due to the small number of tumor cells loaded, cell clusters, overlapping, extrusion, etc. may occur, and it cannot meet the differential diagnosis of immunohistochemistry in the later stage. The number of sliced ​​cells made by the cell wax block is large, can be sliced ​​continuously, and can meet the requirements of immunohistochemistry and molecular pathology, and meet the needs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36
Inventor 赵洁付海洋
Owner QINGDAO MUNICIPAL HOSPITAL
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