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Kit for utilizing combination of four kinds of protein for predicting prognosis of esophagus cancer patient

A kit, technology for esophageal cancer, applied in the field of kits that use 4 proteins to jointly predict the prognosis of patients with esophageal cancer

Inactive Publication Date: 2014-09-10
CHIFENG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The expression of this protein in tumor tissues is poorly studied

Method used

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  • Kit for utilizing combination of four kinds of protein for predicting prognosis of esophagus cancer patient
  • Kit for utilizing combination of four kinds of protein for predicting prognosis of esophagus cancer patient
  • Kit for utilizing combination of four kinds of protein for predicting prognosis of esophagus cancer patient

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Experimental program
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Embodiment Construction

[0019] We collected archival wax blocks of surgical specimens from 299 patients with esophageal cancer from 2007 to 2011. Further detailed description will be given below in conjunction with the embodiments of the accompanying drawings.

[0020] The steps of immunohistochemical hypersensitive two-step method are as follows.

[0021] After the specimens were serially sectioned, dewaxed in xylene, and hydrated with graded alcohol, the expression of the target protein was detected by an ultrasensitive two-step method, and the specific method was as follows.

[0022] (1) The slices were repaired with 0.01M citrate repair solution, 3% H 2 o 2 After inactivating endogenous peroxidase and blocking with goat serum, they were incubated with δ-catenin, Dynamin-2, Erbin, and LAP-1 antibodies overnight at 4°C.

[0023] (2) The sections were incubated with Polymer enhancer and Polymer polymer respectively and then developed by DAB.

[0024] (3) The sections were counterstained with hem...

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Abstract

The to-be solved technical problem of the invention is to provide a reagent for detecting the expression situation of target protein in a paraffin-embedded tissue. The technical scheme for solving the technical problem is to provide a kit for evaluating prognosis information of an esophagus cancer patient. The kit comprises four antibodies of target protein, and the four antibodies comprise delta-catenin, Dynamin-2, Erbin and LAP-1, and the kit also comprises goat serum, 0.01 M citrate restoration liquid, 3% H2O2, a Polymer reinforcing agent, a polymer, a DAB chromogenic reagent and a PBS solution. The kit can help to relatively simply practicably evaluate prognosis of esophagus cancer patients compared with an international TNM staging method, and is higher in both sensitivity and specificity compared with the TNM staging method, and is relatively suitable for esophagus cancer prognosis evaluation of countrymen.

Description

technical field [0001] The invention relates to a kit for combining multiple protein antibodies and using an immunohistochemical hypersensitive two-step method to detect the expression of esophagus cancer patient specimens and then predict the patient's prognosis. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal cancer each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal cancer in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in Henan Province, Yangquan in Shanxi Province, Yanting in Sichuan Province, Chaoshan in Guangdong Province, and Huai'an in Jiangsu Province were established as the six high...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57407G01N33/6893G01N2800/52
Inventor 张俊毅白玉勤瑞云董彩凤许秀娥吴健谊许丽艳李恩民
Owner CHIFENG UNIV
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