Autoantibody detection tissue chip

A tissue chip and autoantibody technology, which is applied in the field of biomedical detection chips, can solve the problems of inability to detect autoantibodies well, the limitation of cultured cells is strong, and the tissue structure is not clear enough, and achieves a simple and feasible preparation method and is easy to commercialize. the effect of a clear organizational structure

Inactive Publication Date: 2003-09-03
陕西超英生物医学研究开发有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention overcomes the difficult preservation of fresh tissue frozen section substrates in the prior art and the high cost, and the tissue structure is not clear enough; the cultured cells have strong limitations and can only be used to detect antinuclear antibodies (such as Hep2 cells), and cannot be used in the original detection and analysis at the same position; the combined detection of the two cannot detect all the shortcomings of autoantibodies well

Method used

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  • Autoantibody detection tissue chip
  • Autoantibody detection tissue chip
  • Autoantibody detection tissue chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, see figure 1 ,

[0034] 1. Anti-double-stranded DNA antibody detection chip:

[0035] The matrix combination of cultured Hep-2 cells and liver tissue is selected for detection matrix. The combination of Hep-2 cells and liver tissue is the standard matrix for detecting double-stranded DNA antibodies. Two liver tissue areas are combined into one detection area, the basic unit area is 0.3cm×0.6cm, the area of ​​Hep-2 cells is 0.3cm×0.3cm, and the number of Hep-2 cells is 5×10 2 ~1×10 3 piece / mm 2 , the size of the liver tissue area is 0.3cm×0.3cm.

[0036] There are 16 basic units arranged on the detection chip, which are arranged into a combination of 4 basic units to form a tissue / cell microarray, which can detect at least 4 different serum samples at a time or set at least 4 different serum dilutions for the same serum. detection. The liver tissue matrix is ​​a paraffin section or a frozen section of fresh liver tissue.

[0037] 2. The production proc...

Embodiment 2

[0052] Example 2, see figure 2 : There are 5 basic units arranged on the detection chip, and each basic unit is arranged from top to bottom in order of Hep-2 cells, monkey liver, rat liver, and human embryonic liver paraffin section combination, and the size of each point is 0.3cm. The distance between each point in the vertical direction is 0.3cm, the distance between each point in the horizontal direction is 0.8cm, and the number of Hep-2 cells is 5×10 2 ~1×10 3 piece / mm 2 , 1 to 3 serums can be detected at a time.

[0053] (2) The basic unit arranged on the organ / tissue-specific antibody detection chip is the same or different single tissue or tissue combination for detection of various autoimmune antibodies. The appearance of each of the above autoantibodies is related to one or more related to an autoimmune disease, or a specific / marker antibody for a certain type of autoimmune disease, this group of chips can be used for clinical assistance in the diagnosis of autoim...

Embodiment 3

[0058] Example 3, see image 3 :

[0059] 1. Production process of anti-desmosome antibody / pemphigus antibody detection chip

[0060] The detection matrix is ​​a combination of human (or rat) esophagus and tongue tissue paraffin sections, which is the standard matrix for detecting double-stranded DNA antibodies. Area (basic unit), the size of the basic unit is 0.3cm×0.6cm, the size of the esophageal tissue area is 0.3cm×0.3cm, and the size of the tongue tissue area is 0.3cm×0.3cm.

[0061] The arrangement of paraffin tissue slices in the detection chip is 4×12 points, each point has a diameter of 0.3 cm, and there are four points in the longitudinal direction, which are esophagus tissue, tongue tissue, esophagus tissue and tongue tissue in sequence, points 1 and 2 They are connected to form a basic unit. The 3rd and 4th points are connected to form a basic unit. The distance between the 2nd and 3rd points is 0.6cm, and there are 12 columns in the horizontal direction. Morph...

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Abstract

A tissue chip for detecting autoantibody is composed of substrate and the matrix on the substrate. Said matrix may be paraffin wax coated tissue, frozen fresh tissue, or cell specimen. An indirect immunofluorescence technique is used to detect, screen and analyze a great deal of autoantibodies in the blood serum of autoimmunopathy patient.

Description

Technical field: [0001] The patent invention relates to a biomedical detection chip, and further relates to an autoantibody detection tissue chip. Background technique: [0002] Autoimmune diseases refer to diseases in which autoimmune reactions cause tissue damage, including more than 30 different diseases in 11 categories, including systemic lupus erythematosus, autoimmune hepatitis, rheumatism and rheumatoid diseases, ulcerative colitis , atrophic gastritis, type I diabetes, myocarditis, cardiomyopathy and malignant tumors are very common diseases, and the diagnosis is more difficult. Among patients with autoimmune diseases, one or more autoantibodies can be detected in the serum or other body fluids of a considerable number of patients, which can provide a very valuable basis for the diagnosis of autoimmune diseases, regardless of clinical diagnosis, treatment and Prognosis follow-up, or research on the mechanism of autoimmune diseases, the research on the method and me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/53G01N33/532G01N33/533G01N33/543G01N33/68
Inventor 李军李晓华卫军霞步雪张美萍李娟
Owner 陕西超英生物医学研究开发有限公司
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