A kind of rapid lysate of human hair for trace drug detection and its application

A lysing solution and hair technology, applied in the preparation of test samples, measuring devices, biological testing, etc., can solve the problems of environmental pollution, influence detection, low release efficiency, etc., and achieve shortened operation time, rapid lysis speed, and accelerated release. effect of the process

Active Publication Date: 2021-11-16
江苏苏博生物医学科技南京有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the background of the acid hydrolysis hair product is low, but the release efficiency of the drug molecule of the test substance is lower than that of the alkaline hydrolysis method; the alkali hydrolysis can completely dissolve the hair, so that the drug molecule to be tested is completely released, but the protein impurity content in the extract high and affect detection
Ultrasonic hydrolysis of organic solvents such as methanol and acetonitrile has problems such as environmental pollution, complicated operation, and difficult promotion at the grassroots level.

Method used

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  • A kind of rapid lysate of human hair for trace drug detection and its application
  • A kind of rapid lysate of human hair for trace drug detection and its application
  • A kind of rapid lysate of human hair for trace drug detection and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Detection of amphetamine-type drugs in the hair of drug-related personnel

[0036] According to the above lysate formula, the hair of drug-related personnel is processed and tested, and the operation steps are as follows:

[0037] Step 1. Hair sample collection: According to the "Standards for the Detection of Hair Samples of Drug-Related Personnel", start collecting from the root of the hair of the monitored person, mark the hair root and hair tail, and use hair 3cm away from the root, 30 hairs, about 5mg;

[0038] Step 2. Physically break the hair sample: use scissors to cut the collected hair sample into thin sections of 0.5mm-1mm to obtain hair sample powder;

[0039] Step 3. Preparation of mixed drug release solution in hair: absorb the lysate at a ratio of 500ul per 5mg of hair sample, put it in a 1.5ml centrifuge tube and mix it with the hair sample powder, and oscillate with ultrasound (frequency 25KHZ, power 300W) for 90 seconds to prepare Obtain dr...

Embodiment 2

[0042] Example 2: Detection of morphine-like drugs in the hair of drug-related personnel

[0043] Step 1. Hair sample collection: According to the "Standards for the Detection of Hair Samples of Drug-Related Personnel", start collecting from the root of the hair of the monitored person, mark the hair root and hair tail, and use hair 3cm away from the root, 30 hairs, about 5mg;

[0044] Step 2. Physically break the hair sample: use scissors to cut the collected hair sample into thin sections of 0.5mm-1mm to obtain hair sample powder;

[0045] Step 3. Preparation of the drug release mixed solution in hair: absorb the lysate at a ratio of 500ul per 5mg of hair sample, put it in a 1.5ml centrifuge tube, mix it with the hair sample powder, and oscillate with ultrasound for 90 seconds to prepare the drug release mixed solution in hair ;

[0046] Step 4: Take 5 drops (about 100uL) of the mixed drug release solution in hair prepared in step 3, drop it vertically into the sample hole ...

Embodiment 3

[0048] Example 3: Detection of ketamine-like drugs in the hair of drug-related personnel

[0049] Step 1. Hair sample collection: According to the "Standards for the Detection of Hair Samples of Drug-Related Personnel", start collecting from the root of the hair of the monitored person, mark the hair root and hair tail, and use hair 3cm away from the root, 30 hairs, about 5mg;

[0050] Step 2. Physically break the hair sample: use scissors to cut the collected hair sample into thin sections of 0.5mm-1mm to obtain hair sample powder;

[0051] Step 3. Preparation of the drug release mixed solution in hair: absorb the lysate at a ratio of 500ul per 5mg of hair sample, put it in a 1.5ml centrifuge tube, mix it with the hair sample powder, and oscillate with ultrasound for 90 seconds to prepare the drug release mixed solution in hair ;

[0052] Step 4: Take 5 drops (about 100uL) of the mixed drug release solution in hair prepared in step 3, and drop it vertically into the sample h...

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Abstract

The invention discloses a human hair rapid lysate for trace drug detection and application thereof. The human hair rapid lyse for trace drug detection has a raw material component comprising: 50-100nM alkaline protease AH-101 , 0.5‑2% thioglycolic acid, 25‑75 mM glycine, 30‑60 mM tris hydrochloride buffer, 40‑80 mM sodium chloride‑sodium hydroxide buffer, and 0.5‑1.5% polyethylene glycol octane phenyl phenyl ether (Triton X-100); wherein, nM is nanomoles per liter, mM is millimoles per liter, and % is volume percent. The present invention can quickly extract trace drug small molecules from human hair samples, has high release rate of drugs, little influence of impurities, non-toxic and pollution; Quickly, the whole cracking time only needs 90 seconds, so that the drug molecules are effectively released, compared with the cracking method in the prior art, the operation time is greatly shortened, and the authenticity and reliability of the experimental results are guaranteed.

Description

technical field [0001] The invention relates to a rapid human hair lysate for trace drug detection and an application thereof, belonging to the field of drug detection in vivo. Background technique [0002] Drug abuse refers to the repeated and massive use of some drugs with dependence or dependence potential irrespective of the medical purpose. The drug users adopt the form of self-administration, leading to mental and physical dependence, causing mental confusion and some abnormal behavior. Substance abuse is also known as "drug use" or "drug abuse." Drug abuse is a major problem that seriously endangers social security and human life and health. According to statistics, the number of new-type drug users in my country increases by about 30% every year, of which young people account for 86%. In order to effectively combat drug crimes and cooperate with law enforcement departments such as public security in drug control work, researchers have established a series of accur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/40G01N1/34G01N33/48
CPCG01N1/34G01N1/4044G01N33/48
Inventor 王宇峰苏雪峰刘洋春
Owner 江苏苏博生物医学科技南京有限公司
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