A kind of rapid lysate of human hair for trace drug detection and its application

Abstract

The invention discloses a human hair rapid lysate for trace drug detection and application thereof. The human hair rapid lyse for trace drug detection has a raw material component comprising: 50-100nM alkaline protease AH-101 , 0.5‑2% thioglycolic acid, 25‑75 mM glycine, 30‑60 mM tris hydrochloride buffer, 40‑80 mM sodium chloride‑sodium hydroxide buffer, and 0.5‑1.5% polyethylene glycol octane phenyl phenyl ether (Triton X-100); wherein, nM is nanomoles per liter, mM is millimoles per liter, and % is volume percent. The present invention can quickly extract trace drug small molecules from human hair samples, has high release rate of drugs, little influence of impurities, non-toxic and pollution; Quickly, the whole cracking time only needs 90 seconds, so that the drug molecules are effectively released, compared with the cracking method in the prior art, the operation time is greatly shortened, and the authenticity and reliability of the experimental results are guaranteed.

Description

technical field [0001] The invention relates to a rapid human hair lysate for trace drug detection and an application thereof, belonging to the field of drug detection in vivo. Background technique [0002] Drug abuse refers to the repeated and massive use of some drugs with dependence or dependence potential irrespective of the medical purpose. The drug users adopt the form of self-administration, leading to mental and physical dependence, causing mental confusion and some abnormal behavior. Substance abuse is also known as "drug use" or "drug abuse." Drug abuse is a major problem that seriously endangers social security and human life and health. According to statistics, the number of new-type drug users in my country increases by about 30% every year, of which young people account for 86%. In order to effectively combat drug crimes and cooperate with law enforcement departments such as public security in drug control work, researchers have established a series of accur...

AI Technical Summary

Problems solved by technology

Under normal circumstances, the background of the acid hydrolysis hair product is low, but the release efficiency of the drug molecule of the test substance is lower than that of the alkaline hydrolysis method; the alkali hydrolysis can completely dissolve the hair, so that the drug molecule to be tested is completely released, but the protein impurity content in the extract high and affect detection

Ultrasonic hydrolysis of organic solvents such as methanol and acetonitrile has problems such as environmental pollution, complicated operation, and difficult promotion at the grassroots level.

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