A method for extracting seawater microbial metagenomics
An extraction method and a metagenomics technology, which is applied in the field of extraction of seawater microbial metagenomics, can solve problems such as the difficulty of extracting DNA, achieve the effects of improving impurity interference, speeding up experiment speed, and increasing DNA concentration
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[0016] A method for extracting seawater microbial metagenomes, first obtaining seawater samples and performing pretreatment, and then extracting the microbial metagenomes. Specific steps include:
[0017] (1) Removal of impurities in seawater: α-cellulose was added to the seawater sample at a mass-volume ratio of 2%, and allowed to stand at room temperature for 0.5 h; a 0.8 um filter membrane was installed on a sterilized filter, connected Turn on the negative pressure pump, pour the seawater sample over the filter, turn on the power of the negative pressure pump, and perform filtration; then replace the 0.2 um filter membrane to filter the filtrate again; collect both filter membranes; Quickly freeze in liquid nitrogen for 1 min, then place at room temperature for 90 s, repeat freezing and thawing 5 times;
[0018] (2) Lysis of seawater microorganisms: Cut the filter membrane into pieces and place in a 5 ml centrifuge tube, then add 250 μl of cell lysate, 40 μl of lysozyme a...
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