The invention relates to the technical field of tissue embryo teaching, and discloses a low-temperature fresh-keeping storage device for tissue embryo teaching, which comprises a cabinet body, a cabinet door adaptively mounted on the cabinet body, at least one base inserted in the cabinet body, at least two carrier plates arranged on the base along the moving direction of the base, and an action mechanism, the top of the carrier plate is provided with at least one vessel containing groove used for fixing vessels containing embryonic tissues. According to the low-temperature fresh-keeping storage device for tissue embryo teaching, when a vessel needs to be stored, all the butt-joint insertion plates on the base can rotate and lift synchronously through the action mechanism when the base is pulled out of the cabinet opening, so that a carrier plate is inserted, and the pulling-out distance of the base relative to the cabinet opening is shortened; the supporting effect between the track surface of the track groove and the base surface in contact with the track groove is reduced, loss between the track surface and the base surface is reduced, damage and deformation are avoided, follow-up use is guaranteed, time and labor are saved, and convenience and rapidness are achieved.