Pretreatment reagent of sample for leukocyte classification and method

Abstract

The invention relates to a pretreatment reagent of a sample for leukocyte classification. The pretreatment reagent contains a hemolytic agent, and an active component of the hemolytic agent is mainlycomposed of organic acid and a polymer non-ionic surfactant; the organic acid selects from at least one of formic acid, acetic acid and propionic acid; the polymer non-ionic surfactant selects from atleast one of polyvinylpyrrolidone and polyethylene glycol. When the pretreatment reagent of the sample is used for treating the sample for the leukocyte classification, the erythrocyte lysis efficiency is high, the leukocyte destructiveness is small, and the treated sample is long in preservation time.

Description

technical field [0001] The invention relates to the technical field of detection and analysis, in particular to a pretreatment reagent and method for a sample for leukocyte classification. Background technique [0002] Flow cytometry (Flow Cytometry, FCM) is an analytical technique developed in the 1970s that integrates multiple disciplines such as optics, fluid, electronics, mechanics, computers, and immunity. At present, it is mainly used in the medical field for the analysis of lymphocyte subsets and the detection of leukemia immunophenotyping and other items. In the analysis of white blood cell classification, clinical blood samples or bone marrow samples often contain a large number of red blood cells, which will increase the background of cell signals and interfere with the classification of white blood cells. Therefore, hemolytic agents should be used to lyse the red blood cells in the sample before analysis. To eliminate the influence of red blood cells on flow cyto...

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In addition, the source of lipoprote

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