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Integrated microfluidic chip for capture of cancer cells in whole blood

A microfluidic chip, cancer cell technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of easy breakage and low selectivity of PDMS, and achieve the advantages of avoiding pollution, simple manufacturing process and less sample usage. Effect

Active Publication Date: 2012-02-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The small slits with a width of 2 μm on the side of the design make PDMS easy to break when demoulding, and the selectivity of the chip to capture cells is not high, and the red blood cells, white blood cells and cancer cells in the whole blood will be captured at the same time

Method used

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  • Integrated microfluidic chip for capture of cancer cells in whole blood
  • Integrated microfluidic chip for capture of cancer cells in whole blood
  • Integrated microfluidic chip for capture of cancer cells in whole blood

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Embodiment

[0039] Such as figure 1 As shown, an integrated microfluidic chip for whole blood cancer cell capture is mainly composed of inlet 1, red blood cell lysing unit 2, white blood cell capture unit 3, cancer cell capture unit 4 and outlet 5.

[0040] There are three inlets 1, which are whole blood inlet, erythrocyte lysate inlet and buffer solution inlet containing leukocyte surface antigen antibody magnetic beads respectively.

[0041] Such as figure 2 As shown, the inlet 1 is a cylindrical cavity structure in PDMS with a diameter of 1.5mm and a height of 3mm.

[0042] The middle inlet of the inlet 1 is 4 mm long, 100 μm wide, and 20 μm high, and the pipes on both sides are 3 mm long, 100 μm wide, and 20 μm high.

[0043] The erythrocyte lysing unit 2 is an S-shaped mixing channel structure.

[0044] Such as image 3 As shown, the S-shaped mixing channel is composed of a plurality of semicircular rings connected end to end, and the radius of the inner semicircle is R 1 150μm...

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Abstract

The invention relates to an integrated microfluidic chip for capture of cancer cells in whole blood. The integrated microfluidic chip for capture of cancer cells in whole blood comprises three inlets, an erythrocyte lysis unit, a leucocyte capture unit, a cancer cell capture unit and an outlet, wherein the three inlets, the erythrocyte lysis unit, the leucocyte capture unit, the cancer cell capture unit and the outlet are connected orderly. The leucocyte capture unit comprises a first diverter, a soft magnetic microcolumn array and permanent magnets. The first diverter is connected with the soft magnetic microcolumn array. The permanent magnets are arranged at two sides of the soft magnetic microcolumn array. The cancer cell capture unit comprises a second diverter and a capture microstructure array, wherein the second diverter and the capture microstructure array are connected orderly. Compared with the prior art, the integrated microfluidic chip for capture of cancer cells in whole blood has the advantages of high integration degree, simple operation, high cell capture efficiency, simple manufacture processes and the like.

Description

technical field [0001] The invention relates to a microfluidic medical diagnosis chip, in particular to an integrated microfluidic chip for whole blood cancer cell capture. Background technique [0002] In today's society, the threats of various diseases, especially AIDS and cancer, seriously threaten human health. Cell research is very important to the research of major diseases such as AIDS and cancer, and providing a convenient and fast cell capture and detection method will promote the development of cell biology. Therefore, it is of great significance to separate, capture and detect cancer cells from whole blood for the study of cancer cell metastasis and postoperative judgment. [0003] With the rapid development of Micro-Electro-Mechanical Systems (MEMS) technology and life sciences, analytical sciences and other disciplines, biological MEMS (Bio-MEMS) technology and Micro Total Analysis System (Micro Total Analysis System) based on Bio-MEMS technology Analysis Syst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
Inventor 陈迪陈景东袁涛陈翔崔大祥
Owner SHANGHAI JIAO TONG UNIV
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