Cell preservation solution for rapid cell immunohistochemistry as well as preparation method and application thereof
A technology of cellular immunity and preservation solution, applied in the field of cytopathological detection, can solve the problems such as immaturity of cellular immunohistochemical technology, difficulty in accurately observing the shape of target cells, and weakening the timeliness of pathological detection results, so as to prevent cell edema, The effect of enhancing timeliness and shortening response time
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Embodiment 1
[0043] Example 1 Preparation of Cell Preservation Solution 1 for Rapid Immunohistochemistry
[0044] For the cell preservation solution 1 for rapid immunohistochemistry provided in this example, weigh or measure each component according to the following table:
[0045] Table 1 Formula of Cell Preservation Solution 1
[0046]
[0047]
[0048] In the above formula, "w / v" or "v / v", "v" in the denominator refers to the total volume of the prepared cell preservation solution; "w" or "v" in the numerator refers to the corresponding group points of mass or volume.
[0049] The preparation method of cell preservation solution 1 for rapid immunohistochemistry provided in this example is as follows:
[0050] 1) According to the formula shown in Table 1, add Tris buffer and NaCl, KCl, MgCl in double distilled water 2 , PEG4000, TritonX-100, EDTA.2NA, TCEP, and get solution A after mixing;
[0051] 2) Add fixative ethylene glycol and methanol to solution A, and mix well to obtain...
Embodiment 2
[0057] Example 2 Preparation of Cell Preservation Solution 2 for Rapid Immunohistochemistry
[0058] For the cell preservation solution 2 for rapid immunohistochemistry provided in this example, weigh or measure each component according to the following table:
[0059] Formulation of table 2 cell preservation solution 2
[0060]
[0061]
[0062] In the above formula, "w / v" or "v / v", "v" in the denominator refers to the total volume of the prepared cell preservation solution; "w" or "v" in the numerator refers to the corresponding group points of mass or volume.
[0063] The preparation method of cell preservation solution 1 for rapid immunohistochemistry provided in this example is as follows:
[0064] 1) According to the formula shown in Table 1, add phosphate buffer, NaCl, KCl, PEG4000, EDTA.2NA and TCEP into double distilled water, and mix well to obtain solution A;
[0065] 2) Add fixative ethylene glycol and ethanol to solution A, and mix to obtain solution B; ...
Embodiment 3
[0071] Example 3 Preparation of Cell Preservation Solution 3 for Rapid Immunohistochemistry
[0072] For the cell preservation solution 2 for rapid immunohistochemistry provided in this example, weigh or measure each component according to the following table:
[0073] Table 3 Formula of Cell Preservation Solution 3
[0074]
[0075]
[0076] In the above formula, "w / v" or "v / v", "v" in the denominator refers to the total volume of the prepared cell preservation solution; "w" or "v" in the numerator refers to the corresponding group points of mass or volume.
[0077] The preparation method of cell preservation solution 1 for rapid immunohistochemistry provided in this example is as follows:
[0078] 1) According to the formula shown in Table 3, add potassium phosphate buffer solution and NaCl, KCl, PEG4000, NH 4 CL, sodium citrate, and sodium mercaptosulfonate were mixed to obtain solution A;
[0079] 2) Add fixatives such as isopropanol and ethanol to solution A, an...
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