Disposable separation chip module for circulating tumor cells in peripheral blood and method of use thereof

Abstract

The invention provides a disposable separating chip module for a peripheral blood circulating tumor cell and an application method of the disposable separating chip module. The disposable separating chip module comprises a micro-fluidic chip, a blood sample storage tank, a PBS sample storage tank, a waste liquid storage tank, an enrichment recycling liquid outlet, a connecting guide pipe (1) of the micro-fluidic chip and the blood sample storage tank, a connecting guide pipe (2) of the micro-fluidic chip and the PBS sample storage tank, a connecting guide pipe (3) of the micro-fluidic chip and the waste liquid storage tank, and a connecting guide pipe (4) of the micro-fluidic chip and the enrichment recycling liquid outlet. The invention further provides the application method of the disposable separating chip module. The micro-fluidic chip adopts a dual-inlet and dual-outlet structure and has an erythrocyte removing efficiency of 100%. A clinical blood sample requires no pretreatment process of attenuation or erythrocyte lysis, so that plenty of time for separating operation can be saved, and the activity of the circulating tumor cell after separation and enrichment can be effectively guaranteed.

Description

technical field [0001] The invention belongs to the technical field of tumor detection, and in particular relates to a disposable separation chip module for circulating tumor cells in peripheral blood and an application method thereof. Background technique [0002] Circulating tumor cells (CTCs) are tumor cells that enter peripheral blood from primary tumor lesions or metastatic lesions. The detection of circulating tumor cells is of great significance to cancer prevention and treatment, and can be effectively applied to in vitro early diagnosis, rapid evaluation of chemotherapy drugs, individualized treatment etc. For routine tumor detection methods, such as imaging, it is difficult to confirm that the tumor is abnormal if it is less than one centimeter. It can be seen from domestic and foreign research that many tumors have tumor cells entering the blood circulation when they are 2-4 mm in size. From this perspective, its significance for early diagnosis cannot be underes...

AI Technical Summary

Problems solved by technology

Because the study uses a single-entry chip, it is difficult to achieve a high recovery rate, and the blood needs to be diluted tenfold, and the separation time will be greatly prolonged; after the diluted blood is passed into the chip, the red blood cells and white blood cells are evenly distributed throughout the chip Yes, so the ratio of red blood cells coming out of the collection port of the enriched solution is the same as the dilution ratio of the blood, and the concentration of red blood cells in the final collection solution is the same as the concentration of blood dilution

Containing red blood cells in the enrichment solution will have a great impact on the observation, affecting the purity of circulating tumor cells, and thus adversely affecting the analysis and research of circulating tumor cells

Images