Bone marrow peptide extraction and preparation method

Abstract

The invention discloses a bone marrow peptide extraction and preparation method, which comprises: taking a fresh bone, removing connective tissue, filling the bone marrow into a centrifugation vessel, carrying out ultrasonic treatment on the treated solution to break cells, carrying out centrifugation to remove the supernatant, adding an erythrocyte lysis buffer and a sterile PBS buffer solution, and carrying out centrifugation to obtain the bone marrow peptide. And performing continuous graded gradient ultrafiltration on the mixed solution to prepare a concentrated raw material solution, and preparing different preparations of the bone marrow peptide according to the concentration requirement. According to the method, bone marrow peptide extraction can be effectively carried out on various materials. Meanwhile, the extraction rate of bone marrow peptide is greatly increased, the activity of bone marrow peptide can be reserved through ultrasonication, the denaturation trend of protein is effectively avoided. Meanwhile, the production time is shortened; the red blood cell lysis buffer is matched with the PBS buffer solution, so that the generation rate of the peptide can be further improved, the bone marrow peptide with the highest quality can be obtained under the filtration of an ultrafilter with an ultrafiltration membrane, and the influence of the molecular weight distribution of the polypeptide can be reduced through the spray drying process and the control of the temperature of inlet air and outlet air.

Description

technical field [0001] The invention relates to the technical field of polypeptide extraction and preparation, in particular to a method for extracting and preparing bone marrow peptides. Background technique [0002] Small molecular peptides can strengthen the reproduction of beneficial bacteria in the digestive tract, improve the synthesis of bacterial proteins, and enhance the body's disease resistance; endogenous peptides such as interferon and interleukin can stimulate and regulate the immune response center, and have immune activity. In addition, some small molecular peptides (such as Exorphines) can make the small intestine of small animals mature in advance, stimulate the secretion of digestive enzymes, and improve the body's immunity. [0003] In addition to being a donor of amino acids and promoting production performance with its absorption mechanism, peptides also serve as physiological regulators. They can directly act as neurotransmitters or indirectly stimulat...

AI Technical Summary

Problems solved by technology

However, the traditional methods for determining available amino acids just ignore the important physiological effects of small molecular peptides on animals.

[0004] Not only that, when people or animals lose a lot of glutamic acid / glutamine due to injury, long-term disease and sepsis, the degradation of muscle protein is accelerated. When glutamic acid is supplied parenterally in the form of peptides to surgical patients, It can partially reduce the negative nitrogen balance and prevent the loss of muscle amino acids, but the free form of glutamic acid has no such effect; most of the peptides are extracted from the bone marrow of animals. At present, the extraction rate of bone marrow peptides is low, and the material Single, resulting in higher production cost of bone marrow peptide