Aspergillus quantitative detection fluorescence PCR (polymerase chain reaction) kit

Abstract

The invention relates to an Aspergillus quantitative detection fluorescence PCR (polymerase chain reaction) kit which comprises a positive work standard substance, a negative work standard substance, a detection reaction solution, an erythrocyte lysis solution, a dilute solution and a DNA (deoxyribonucleic acid) polymerase, wherein the positive work standard substance, negative work standard substance and detection reaction solution respectively contain specific primers and a specific probe; and the forward sequence of the specific primer is SQ1 or complementary strand thereof, the reverse sequence is SQ2 or complementary strand thereof, and the sequence of the specific probe is SQ3 or complementary strand thereof. The kit is quantitative and accurate, has the advantages of high sensitivity in PCR, specificity in DNA hybridization and high accuracy and quantitativeness in the spectral technique, has the characteristics of visual result and high detection speed, and can directly detect the changes in the PCR process. Compared with the common PCR, the result of the kit can be observed in real time, the product does not need gel electrophoresis detection, and the operation is completely performed in a closed pipe mode, thereby effectively lowering the pollution.

Description

technical field [0001] The invention belongs to the technical field of in vitro nucleic acid diagnostic reagents, in particular to a fluorescent PCR kit for quantitative detection of aspergillus. Background technique [0002] Aspergillus is a typical filamentous fungus that exists widely in the environment. It enters the human body mainly through the inhalation of mold spores through the respiratory tract. It first invades and infects the lungs and causes pulmonary aspergillosis. Aspergillus has become an important pathogenic fungus next to Candida albicans in immunosuppressed patients with blood system diseases, tuberculosis, chronic bronchitis, bronchiectasis, and lung cancer. Aspergillus infection has become one of the most common and widely spread opportunistic pathogens, but early diagnosis is difficult, which often leads to delay in treatment, and the fatality rate is as high as 30% to 50%. Traditional diagnostic methods such as fungal isolation, culture and histopath...

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Problems solved by technology

[0004] Aspergillus fumigatus is the most common infection in Aspergillus fumigatus, but currently there is no fluorescent PCR product for detecting Aspergillus fumigatus in China, let alone a wider range of fluorescent PCR detection products for Aspergillus fumigatus

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