Double-modified engineering bacterium and application thereof

A double modification and engineering technology, applied in the fields of application, bacteria, genetic engineering, etc., can solve the problems of poor permeability and difficulties, and achieve the effects of prolonging survival, good permeability, and inhibiting tumor growth
CN114196604APending Publication Date: 2022-03-18RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
Publication Date
2022-03-18

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Abstract

The invention belongs to the field of biological medicines, and relates to a dual-modified engineered bacterium and application thereof, in particular to an engineered bacterium externally coupled with an immune checkpoint inhibitor and internally coded with a tyrosinase gene, a pharmaceutical composition containing the dual-modified engineered bacterium and application of the pharmaceutical composition. Wherein the engineered bacterium is preferably escherichia coli. By utilizing the engineered bacteria, melanin expression and distribution which are controllable in space and time are obtained, melanin is similar to an inhibitor in distribution, and the melanin and the inhibitor are combined to enhance a dual-immune activation effect and synergistically reprogram a tumor immune microenvironment. The engineered bacterium can significantly inhibit tumor growth and prolong the lifetime by being singly used or combined with a conventional tumor treatment mode or medicine. The pharmaceutical composition containing the bacteria or the application of the pharmaceutical composition not only overcomes the difficult tumor inner barrier, but also has good permeability, and can uniformly and enduringly disperse the anti-tumor drug into the whole tumor.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and relates to an engineered bacterium encoding a tyrosinase gene, in particular to a double-modified engineered bacterium and its application. The bacterium is an externally coupled immune checkpoint inhibitor and an internally An engineered bacterium encoding a tyrosinase gene, a pharmaceutical composition containing the bacterium and applications thereof. Background technique

[0002] In terms of cancer therapy, approaches that enable the uniform and durable distribution of anticancer drugs throughout tumors are of great interest for maximizing their therapeutic efficacy, but have proven to be extremely challenging. At present, anticancer drugs have limited accumulation in tumor tissue and poor penetration ability, which often affects their curative effect. Various mildly loaded nanoparticles have been developed for concentrating drugs at tumor sites through the high-osmotic long-retention EPR effe...

Claims

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