Bionic degradable artificial nerve conduit for regulating and controlling immune microenvironment and guiding regeneration by utilizing topological structure, and preparation method of bionic degradable artificial nerve conduit

A topological structure and guided regeneration technology, applied in the field of biomimetic degradable artificial nerve conduits and its preparation, can solve the problems of mismatching degradation speed, human body's own repair speed, inability to stimulate self-remodeling potential, lack of bionic design, etc., to promote migration and differentiation, prevention of scar tissue formation, and prevention of cast collapse

Inactive Publication Date: 2020-05-05
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of the lack of bionic design, the mismatch between the degradation speed and the human body's own repair speed, and the inability to stimulate the potential of self-remodeling in the existing nerve conduit products on the market

Method used

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  • Bionic degradable artificial nerve conduit for regulating and controlling immune microenvironment and guiding regeneration by utilizing topological structure, and preparation method of bionic degradable artificial nerve conduit
  • Bionic degradable artificial nerve conduit for regulating and controlling immune microenvironment and guiding regeneration by utilizing topological structure, and preparation method of bionic degradable artificial nerve conduit
  • Bionic degradable artificial nerve conduit for regulating and controlling immune microenvironment and guiding regeneration by utilizing topological structure, and preparation method of bionic degradable artificial nerve conduit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A method for preparing a biomimetic and degradable artificial nerve guide that regulates the immune microenvironment and guides regeneration by using topological structure, comprising the following steps:

[0066] Step 1, preparing the shell: using the first material to prepare by electrospinning;

[0067] Step 1.1, select 1g of PLCL as the first material, mix it with a solvent and stir overnight at room temperature to dissolve, and prepare a solution with a concentration (solute mass to solvent volume ratio) of 10 g / ml;

[0068] Step 1.2, mount a 3 mm diameter stainless steel receiving rod on the electrospinning machine and ground it, draw the PLCL solution described in step 1.1 into the syringe, install the syringe on the syringe pump, and place the syringe needle at a distance of 15 cm from the receiver , use a high-voltage DC power supply to apply a voltage of 16kV to the needle;

[0069] Step 1.3, set the advance speed of the syringe pump to 4ml / h, the rotational ...

Embodiment 2-4

[0076] The preparation method is the same as in Example 1, only the preparation parameters are adjusted. See Table 1-2 for specific parameters.

[0077] Table 1 Shell preparation condition table described in embodiment 1-4

[0078]

[0079] Table 2 Table of conditions for preparing inner fibers described in Examples 1-4

[0080]

[0081] For the product that embodiment 1-4 obtains, tested, test result is as follows Figure 2-9 , it can be seen that the product of the present invention can achieve the effect of guiding nerve conduit regeneration.

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Abstract

The invention belongs to the field of tissue engineering, and particularly relates to a bionic degradable artificial nerve conduit for regulating and controlling immune microenvironment and guiding regeneration by utilizing a topological structure. The bionic degradable artificial nerve conduit comprises a shell (1) and inner fibers (2), wherein the shell (1) adopts a first material with degradation time of 12-24 months; and the inner fibers (2) are made of a second material with the degradation time of 3-10 months. The bionic degradable artificial nerve conduit has the beneficial effects thatthe macrophages can be regulated and controlled by the internal fibers, so the macrophages are polarized into M2 phenotypes for promoting tissue regeneration, and nerve regeneration is further promoted; the degradation speed of the shell is matched with a nerve scar formation period, so infiltration of surrounding tissue is effectively prevented, and formation of scar tissue is avoided; and the shell is provided with pores, so nutrient exchange and substance transfer of nerve cells in the regeneration process are not influenced.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a bionic degradable artificial nerve guide which utilizes topological structure to regulate immune microenvironment and guide regeneration and a preparation method thereof. Background technique [0002] As a country with a large population, with the growth of China's and the world's economy, the improvement of the quality of life and the enhancement of health awareness, the medical needs for severe trauma treatment and chronic degenerative diseases are increasing. According to the 2018 "National Health Data Report", the number of hospitalized patients with peripheral nerve injuries due to accidental injuries, accidents and other joint trauma accounted for 9% of the total number of hospitalizations, ranking third only in respiratory diseases ( 12%) and gastrointestinal diseases (10%). According to the relevant information disclosed in the "National Key Basic Research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/40A61L27/50A61L27/58A61F2/02
CPCA61L27/18A61L27/50A61L27/58A61F2/02C08L67/04
Inventor 董显豪孔德领朱美峰
Owner NANKAI UNIV
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