Application of galangin or salt thereof to preparation of medicines or health-care products capable of preventing and treating lung fibrosis
A technology of pulmonary fibrosis and galangin, applied in the field of medicine, can solve problems such as increased risk of respiratory failure, decreased immunity, liver and kidney damage, etc., and achieves significant clinical and social significance, good curative effect, and low toxicity.
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Embodiment 1
[0029] Example 1 In vitro cell experiment of galangin preventing and treating pulmonary fibrosis
[0030] The excessive proliferation of mouse embryonic fibroblasts (NIH3T3) and pancreatic cancer human alveolar basal epithelial cells (A549) can cause collagen deposition, therefore, inhibiting their proliferation can inhibit the expression and deposition of collagen.
[0031] In the process of pulmonary fibrosis, TGF-β is the main fibrosis factor, which can promote epithelial-mesenchymal transition (EMT) in vitro, inhibit the apoptosis of fibroblasts, and promote the production of reactive oxygen species; Studies have also shown that the massive release of TGF-β will accelerate the process of pulmonary fibrosis. After A549 is stimulated by TGF-β1, it will accelerate the process of epithelial-mesenchymal transition (EMT), and then accelerate the formation of pulmonary fibrosis. Therefore, inhibiting the transformation of EMT can reduce the formation of pulmonary fibrosis.
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Embodiment 2
[0045] Example 2 In vivo animal experiment of galangin preventing and treating bleomycin-induced pulmonary fibrosis
[0046] Bleomycin (BLM) is a drug against squamous cell carcinoma, but it can cause pulmonary fibrosis. After bronchial infusion of bleomycin, type Ⅰ alveolar epithelial cells secrete pro-inflammatory factors after inflammatory injury, the proliferation and differentiation of type Ⅱ epithelial cells can be observed on the second day, myofibroblasts are recruited, synthesize collagen and Cause extracellular matrix accumulation, resulting in tissue and interstitial fibrosis. This is consistent with the induction of diffuse pulmonary fibrosis or fibrotic alveolitis in humans, suggesting that bleomycin-induced injury may provide a suitable model for this disease of unknown etiology and pathogenic mechanism. Based on this, this experiment established a bleomycin-induced pulmonary fibrosis model (CNV) in C57BL / 6 mice to evaluate the effect of galangin on pulmonary fi...
Embodiment 3
[0063] The in vitro safety evaluation of embodiment 3 galangin
[0064] 1. Cell Culture
[0065] Human normal hepatocytes (LO 2 ) was cultured with 10% FBS DMEM high-glucose medium, and 100 U / mL of penicillin and streptomycin were needed to be added during the cultivation and experiment process, and the environment conditions were 37°C, 5% CO 2 in the incubator.
[0066] 2. MTT method detects the effect of galangin on normal human liver cells (LO 2 ) Proliferation
[0067] Select cells in good logarithmic growth state, inoculate a 96-well plate according to the cell growth rate, about 1500-2000 cells per well, 100 μL per well, at 37 ° C, 5% CO 2 Incubator cultivation. On the second day after inoculation, add 100 μL of galangin dilution prepared with fresh medium to each well to make the final concentration 0 μmol / L, 6.25 μmol / L, 12.5 μmol / L, 25 μmol / L, 50 μmol / L, 100 μmol / L L, set 5 duplicate wells at the same concentration, at 37°C, 5% CO 2 After culturing in the incub...
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