Authenticity evaluating method for populus-type propolis based on multi-index quality control

An evaluation method and technology of authenticity, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of good separation effect, high sensitivity and strong feasibility

Inactive Publication Date: 2013-09-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant established a method for distinguishing propolis from poplar gum based on salicin in the previous research, which solved the problem of whether poplar gum is contained in the sample to be tested. However, since this method is unique to poplar gum and propolis does not contain As a reference, it is unavoidable to use non-poplar gum as propolis or adulterate propolis, and then artificially add flavonoids such as rutin to meet the content requirements of "total flavonoids". It is urgent to establish the authenticity evaluation of propolis The system promotes the healthy development of the propolis industry

Method used

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  • Authenticity evaluating method for populus-type propolis based on multi-index quality control
  • Authenticity evaluating method for populus-type propolis based on multi-index quality control
  • Authenticity evaluating method for populus-type propolis based on multi-index quality control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Weigh respectively caffeic acid, ferulic acid, p-coumaric acid, cinnamic acid, rutin, myricetin, apigenin, galangin, chrysin, CAPE, pinenin reference substances 10, 10, 10, 10, 40, 40, 10, 10, 40, 20, 10 mg, dilute to 10 mL with methanol. Then take 0.5, 0.4, 0.3, 0.3, 0.5, 0.5, 2, 2, 0.5, 1.0, 2.0 mL respectively as the standard mixture, filter through a microporous membrane, and then use high performance liquid chromatography for determination. See results Figure 1b , in the figure 1, caffeic acid; 2, p-coumaric acid; 3, ferulic acid; 4, rutin; 5, myricetin; 6, cinnamic acid; 10, galangin.

[0028] 10 g of propolis was taken, frozen, pulverized and mixed, extracted with absolute ethanol ultrasonically, the ratio of solid to liquid was 1:30, ultrasonically extracted twice for 45 min, filtered, and rotary evaporated to obtain the extract. Take 10 mg of propolis extract respectively, ultrasonically dissolve it in methanol to a constant volume, filter it through a micr...

Embodiment 2

[0031] Weigh respectively caffeic acid, ferulic acid, p-coumaric acid, cinnamic acid, rutin, myricetin, apigenin, galangin, chrysin, CAPE, pinenin reference substances 10, 10, 10, 10, 40, 40, 10, 10, 40, 20, 10 mg, dilute to 10 mL with methanol. Then take 0.5, 0.4, 0.3, 0.3, 0.5, 0.5, 2, 2, 0.5, 1.0, 2.0 mL respectively as the standard mixture, filter through a microporous membrane, and then use high performance liquid chromatography for determination. See results Figure 2b , in the figure 1, caffeic acid; 2, p-coumaric acid; 3, ferulic acid; 4, rutin; 5, myricetin; 6, cinnamic acid; 10, galangin.

[0032] Take 10 g of poplar gum, freeze and pulverize it, and then extract it ultrasonically with anhydrous ethanol at a ratio of solid to liquid of 1:30. Ultrasonic for 45 min, extract twice, filter, and rotary evaporate to obtain the extract. Take 10 mg of poplar gum extract respectively, dissolve it with methanol to a constant volume, filter it through a microporous membrane,...

Embodiment 3

[0035] 10 g of poplar propolis from different sources were taken, frozen, pulverized and mixed, then ultrasonically extracted with absolute ethanol at a solid-liquid ratio of 1:30, ultrasonically extracted twice for 45 min, filtered, and rotary evaporated to obtain the extract. Take 10 mg of propolis extract from different sources, dissolve it in methanol to a constant volume, filter it through a microporous membrane, and measure it with high performance liquid chromatography. see image 3 , in the figure a, Liaoning; b, Sichuan; c, Shandong; d, Shaanxi; e, Anhui.

[0036] The conditions of the liquid chromatograph were: Sepax C18 (150 mm×4.6 mm, 5 μm); flow rate 1.0 mL / min; detection wavelength 280 nm; column temperature 30°C; injection volume: 2 μL. The mobile phase was pure methanol and 0.5% acetic acid water mixture by volume ratio, followed by binary gradient elution by volume ratio, and the elution program was: 0~25 min, methanol 20~40%; 25~55 min, methanol 40~55 %; 55...

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Abstract

The invention provides an authenticity evaluating method for a populus-type propolis based on multi-index quality control, and caffeic acid, p-coumaric acid, ferulic acid, rutin, myricetin, cinnamic acid, galangin, chrysin, pinocembrin and CAPE in the propolis are detected by high performance liquid chromatography. A mixing standard sample of caffeic acid, p-coumaric acid, ferulic acid, rutin, myricetin, cinnamic acid, galangin, chrysin, pinocembrin and CAPE is taken as a reference index in the method; wherein whether the propolis is from a populus-type material is defined by the existence or inexistence of cinnamic acid, galangin, chrysin, pinocembrin and CAPE, a poplar tree's gum and the propolis are distinguished by the existence or inexistence of caffeic acid, p-coumaric acid and ferulic acid, adulteration or no adulteration of rutin or myricetin is determined by taking rutin or myricetin as a reference; and thus a detection method for distinguishing between the poplar tree's gum and the populus-type propolis, and adulteration or no adulteration of rutin or myricetin, is constructed, and the method helps to evaluate whether a sample is from populus plants, and helps to achieve discarding of the false and retaining of the true about the populous-type propolis.

Description

technical field [0001] The invention belongs to the field of quality control of bee products, and relates to a method for evaluating the authenticity of Populus type propolis based on multi-index quality control. Background technique [0002] Propolis has been used in natural medicine since ancient times. It is called "black wax" in the ancient Arabic medical book "Medical Code", and it is also one of the main components of dew honeycomb that was first recorded in "Shen Nong's Materia Medica". Propolis itself is a drug, which has been included in the ninth volume of "Chinese Materia Medica", and has been included in the "Pharmacopia of the People's Republic of China" (2005, 2010 edition, one part). Due to the multiple pharmacological activities of propolis, coupled with the enhancement of people's awareness of health care, propolis products are more and more favored by consumers, and have been widely used in medicine, health food, daily chemical and other industries, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张翠平胡福良黄帅
Owner ZHEJIANG UNIV
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