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Method for extracting and detecting flavonoids from galangal

An extraction method and detection method technology, applied in the field of natural compound extraction, can solve the problems of increased safety hazards, increased steps, residues, etc., and achieve the effects of good repeatability, high sensitivity, simple and efficient operation

Inactive Publication Date: 2019-02-22
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In this field, organic reagents are mostly used for the extraction of active ingredients in galangal. The extracts are directly applied to oral preparations to increase the subsequent steps of solvent removal, and it is easy to cause residues and increase potential safety hazards.

Method used

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  • Method for extracting and detecting flavonoids from galangal
  • Method for extracting and detecting flavonoids from galangal
  • Method for extracting and detecting flavonoids from galangal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Test method

[0053] 1.1 Pretreatment of galangal

[0054] Put the purchased galangal into a grinder for crushing, and then pour it into different purpose sieves to sieve. Four different purpose samples were obtained sequentially: 41-51 mesh, 51-69 mesh, 69-91 mesh, and less than 91 mesh. Theoretically speaking, the finer the particle size of the medicinal material, the better the extraction effect, but in reality, the particle size of the traditional Chinese medicinal material is too fine, which will increase the difficulty of subsequent operations during the experiment. Therefore, this application uses galangal less than 51 mesh for the experimental operation.

[0055] 1.2 Preparation of standard solution

[0056] Weigh 0.001g standard samples of each of quercetin, joconin, isorhamnetin, kaempferol, kaempferol, curcumin, and galangin, dissolve them in methanol, and set the volume to a 10mL colorimetric tube. Shake well and perform ultrasonic treatment. After the...

Embodiment 2

[0074] Select less than 51 mesh, weigh about 0.2000g of galangal crushed sample, add it to the subcritical extraction reaction kettle, then add 4.5mL deionized water, react at 130°C for 10min, open the reaction kettle and rinse the inner liner with methanol several times The solution. Pour the rinsed solution completely into a 10mL colorimetric tube and dilute to 10mL with methanol. After shaking well and standing still, take the supernatant, filter through a 0.45 μm water film membrane to obtain a sample solution, and perform HPLC detection. The test results showed that the extraction rate of quercetin was 0.0060, that of kaempferol was 0.0134, that of isorhamnetin was 0.0026, that of chopinenin was 0.0199, that of galangin was 0.4223, that of kaempferol was 0.0875, and that of curcumin was 0.0016.

Embodiment 3

[0076] Select less than 51 mesh, weigh about 0.2000g of galangal crushed sample, add it to the subcritical extraction reaction kettle, then add 4.5mL deionized water, react at 140°C for 10min, open the reaction kettle and rinse the inner tube with methanol several times The solution. Pour the rinsed solution completely into a 10mL colorimetric tube and dilute to 10mL with methanol. After shaking well and standing still, take the supernatant, filter through a 0.45 μm water film membrane to obtain a sample solution, and perform HPLC detection. The test results showed that the extraction rate of quercetin was 0.0068, that of kaempferol was 0.0131, that of isorhamnetin was 0.0049, that of chopinenin was 0.0191, that of galangin was 0.3465, that of kaempferol was 0.0702, and that of curcumin was 0.0010.

[0077] Example 3

[0078]Select less than 51 mesh, weigh about 0.2000g of galangal crushed sample, add it to the subcritical extraction reaction kettle, then add 4.5mL deionized...

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Abstract

The invention belongs to the field of natural compound extraction, and particularly relates to a method for extracting and detecting flavonoids from galangal. In the prior art, subcritical extractionof the flavonoids in galangal mostly adopts an organic solvent as an extraction reagent or is combined with ultrasonic extraction. The method only adopts subcritical water as the extraction reagent, and studies the optimum preparation conditions for extraction of seven substances of quercetin, pinocembrin, isorhamnetin, kaempferol, kaempferide, galangin and curcumin. A galangal extract obtained bythe preparation method can be directly applied to an oral preparation due to the fact that an organic reagent is not introduced during the preparation process. In addition, a high-performance liquidphase detection condition for the galangal extract is provided, and can simultaneously detect flavonoid compounds, and the repeatability is good. The extract and the method are applied to the preparation of cardiovascular drugs and have important production significance.

Description

technical field [0001] The invention relates to the field of natural compound extraction, in particular to a method for extracting flavonoids from galangal by subcritical water, an extract and a high-efficiency liquid phase detection method. Background technique [0002] Galangal is the dried rhizome of galangal (Alpinia officinarum Hance), a plant of the ginger family. It is fragrant, pungent and hot, and returns to the spleen and stomach meridian. It has the functions of warming the stomach, relieving vomiting, dispersing cold and relieving pain. Stomach cold vomiting, belching acid regurgitation embolism. Modern pharmacological studies have shown that galangal also has antibacterial, antiviral, antitumor, antioxidative, anti-gastrointestinal bleeding, anti-ulcer and gastric mucosal protection effects. In recent years, researchers at home and abroad have done a lot of research on the flavonoid components and pharmacological effects of galangal. Several types of flavonoids...

Claims

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Application Information

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IPC IPC(8): B01D11/02G01N30/02G01N30/06
CPCB01D11/02G01N30/02G01N30/06G01N2030/146
Inventor 程燕薛富民王学重刘伟王岱杰赵辉
Owner SHANDONG ANALYSIS & TEST CENT
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