Method for extracting propolis step by step with subcritical fluid

A subcritical fluid and extraction technology, applied in solid solvent extraction, food science, etc., can solve the problems of incomplete extraction of active components, excessive heavy metal content, deep color of the extract, etc., to reduce extraction costs, low operating costs, The effect of low extraction temperature

Active Publication Date: 2017-06-13
江苏鸿祺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional extraction methods of propolis mainly include: water extraction, alcohol extraction, propylene glycol extraction, macroporous resin adsorption, supercritical carbon dioxide extraction, etc. The extracted propolis contains a large amount of inactive components beeswax, and in different degrees There are problems such as complex process, high operating cost, incomplete extraction of active components, deep color of the extract, and excessive heavy metal content.

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  • Method for extracting propolis step by step with subcritical fluid

Examples

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Effect test

example 1

[0024] Weigh 1000g of fur glue (content: 40.7%), freeze at -20°C for 48 hours and pulverize to 0.1-2 mm coarse particles; put the crushed fur glue into a 300-mesh silk bag, tie the mouth, put Put it into the extraction kettle of the subcritical extraction device, vacuumize, add 5200 mL of liquefied butane gas, extract 3 times at 23 ° C, and extract for 40 minutes each time; the extract is transferred to the evaporation tank 2 for evaporation, compressed and cooled by the compressor Then put it in the solvent storage tank 4 for recycling; desolvate the evaporation tank 2 for 40 minutes under negative pressure of -0.09MPa, take out the extract, and obtain 186g of beeswax. Precipitate and vacuumize the extraction tank 2, add 6100 mL of liquefied tetrafluoroethane, extract 3 times at 30°C, and extract for 50 minutes each time; transfer the extract to the evaporation tank 2, evaporate, and desolventize under negative pressure to obtain light-colored The refined propolis extract was...

example 2

[0026] Weigh 1000g of fur glue (content: 23.4%), freeze at -20°C for 48 hours and pulverize to 0.1-2 mm coarse particles; put the crushed fur glue into a 300-mesh silk bag, tie the mouth, put Put it into the extraction kettle of the subcritical extraction device, vacuumize, add 3100 mL of liquefied propane gas, and extract 3 times at 20°C for 30 minutes each time; the extract is transferred to the evaporation tank 2 for evaporation, compressed and cooled by the compressor Put it in the solvent storage tank 4 for recycling; desolvate the evaporation tank 2 for 20 minutes under negative pressure of -0.09MPa, take out the extract, and obtain 107g of beeswax. Desolventize in extraction tank 2, vacuumize, add 2100 mL of liquefied tetrafluoroethane, extract 3 times at 30°C, and extract for 40 minutes each time; transfer the extract to evaporation tank 2, evaporate, and desolventize under negative pressure to obtain light-colored The refined propolis extract was 125g, and the total f...

example 3

[0028] Weigh 500g of fur glue (content: 68.8%), freeze at -20°C for 48 hours and pulverize to 0.1-2 mm coarse particles; put the crushed fur glue into a 300-mesh silk bag, tie the mouth, put Put it into the extraction kettle of the dual-solvent subcritical extraction device, vacuumize, add 5000 mL of liquefied butane gas, extract 3 times at 25 ° C, and extract for 60 minutes each time; the extract is transferred to evaporation tank 2 for evaporation, and compressed by a compressor 1. After cooling, put it in the solvent storage tank 4 for recycling; desolventize the evaporation tank 2 for 60 minutes under negative pressure of -0.09MPa, take out the extract, and obtain 157g of beeswax. Desolventize in extraction tank 2, vacuumize, add 4000 mL of liquefied dimethyl ether, extract 4 times at 55°C, and extract for 70 minutes each time; transfer the extract to evaporation tank 2, evaporate, and desolventize under negative pressure to obtain light-colored refined The propolis extrac...

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Abstract

The invention discloses a method for extracting propolis step by step with subcritical fluid with an aim to extract beewax and propolis from glue respectively. The method includes: performing freezing and breaking on the glue; loading broken glue raw materials in to a sieving cloth bag which is put into an extraction pot for vaporization and adding a primary extractant for extraction; moving the extractant to an evaporating pot for solvent evaporation and placing the vaporized primary extractant in a solvent tank after cooling and liquidation; subjecting the evaporating pot to negative press separation to take out an extractant to obtain the beewax; after the extraction pot is subjected to solvent removal, adding a secondary extractant for extraction; moving the secondary extractant to the evaporating pot for solvent evaporation and placing the vaporized second extractant to the solvent tank after cooling and liquidation; subjecting the evaporating pot to negative press desolventizing to obtain the light-colored refined propolis extract. The method is low in extraction temperature, capable of reducing damage of active components, simple in process, low in extraction cost, high in extraction rate, high in total flavonoid content, high in galangin and complete in extraction of aspen, free from heavy metal in the propolis extraction and solvent residue free.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, in particular to a method for subcritical fluid stepwise extraction of propolis. Background technique [0002] In recent years, propolis has been a hot spot in the research and development of bee products, and its broad-spectrum biological activity has been widely used in modern medicine. [0003] Propolis is a jelly made by mixing the resin collected by bees from plant spores or tree trunks with the secretions of their palate glands and wax glands. 10,000 bees can only collect about 10-12g of propolis a year, known as "purple gold". Propolis has a special aromatic smell, is yellowish brown to dark brown, and can be used as medicine. Propolis contains complex components, including flavonoids, acids, alcohols, phenols, aldehydes, esters, ethers, alkenes, terpenes, steroids, and various amino acids, fatty acids, enzymes, vitamins, and various trace elements. Flavonoids account...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02A23L21/20
CPCB01D11/02
Inventor 张勇王国喜刑正朱新亮
Owner 江苏鸿祺生物科技有限公司
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