Application of carbendazim and galangin compound bactericide in citrus rot prevention and fresh keeping

A compound fungicide, galangin technology, applied in the application, fruit and vegetable preservation, food preservation and other directions, can solve problems such as reduction, and achieve the effects of convenient use, prolonging the fresh-keeping period, and reducing the dosage used

Active Publication Date: 2018-12-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no specific application of imidamide, carbendazim and flavonoid compound fresh-keeping research at present, so the present invention uses imidamide, carbendazim, galangin, pinenin, and chrysin as raw materials to utilize the compound to extract citrus fruit. Based on the antibacterial activity of Penicillium italicum, a composite preservative was prepared to reduce the amount of fungicides imidamide and carbendazim, and an antiseptic and fresh-keeping method that can effectively prolong the postharvest shelf life of citrus was developed

Method used

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  • Application of carbendazim and galangin compound bactericide in citrus rot prevention and fresh keeping
  • Application of carbendazim and galangin compound bactericide in citrus rot prevention and fresh keeping
  • Application of carbendazim and galangin compound bactericide in citrus rot prevention and fresh keeping

Examples

Experimental program
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Effect test

Embodiment 1

[0018] A kind of application of carbendazim and galangin compound bactericide in citrus antisepsis and fresh-keeping, its steps are:

[0019] (1) Configuration of carbendazim mother solution: weigh 0.6892g of carbendazim powder (50% wettable powder), dilute 2500 times with sterile water to obtain 137.84 μg / mL carbendazim mother solution;

[0020] (2) Configuration of galangin mother liquor: Weigh 0.385g of galangin (99%), add 1mL of dimethyl sulfoxide to dissolve, and dilute to 50mL with sterile water to obtain 0.0077g / mL of galangin Mother liquor;

[0021] (3) Configuration of carbendazim and galangin compound fungicide: the carbendazim mother liquor of step (2) and the galangin mother liquor of step (3) are mixed in an equal volume ratio to obtain carbendazim and galangin element compound fungicide, wherein, the concentration of carbendazim is: 68.92 μg / mL, and the concentration of galangin is: 0.00385 g / mL;

[0022] (4) Apply carbendazim and galangin compound fungicide on...

Embodiment 2

[0025] The present embodiment studies the minimum inhibitory concentration of 2 kinds of bactericides to Penicillium italicum:

[0026] The two fungicides were carbendazim and imidamide.

[0027] For each compound fungicide, 10 concentrations were set, and the lowest inhibitory concentration was determined respectively. Select 2 mL of PDA medium with medicine and spread it on the glass slide. After the medium on the glass slide is solidified, absorb a drop of Penicillium italicum spore suspension (concentration: 5×10 6 cfu / mL) was dropped on the medium slide, and then the slide was placed in a petri dish, repeated three times, and cultured at a constant temperature of 26°C for about 10h to observe the spore germination.

[0028] The minimum inhibitory concentrations of the two fungicides are listed in Table 1.

[0029] Table 1 Determination results of minimum inhibitory concentrations of two fungicides

[0030]

Embodiment 3

[0032] This embodiment studies the minimum inhibitory concentration of 3 different types of compound fungicides on Penicillium italicum:

[0033] The three compound fungicides were: carbendazim and galangin compound fungicide, imidamide and pinusin compound fungicide, imidamide and galangin compound fungicide.

[0034] For each compound fungicide, 10 concentrations were set, and the lowest inhibitory concentration was determined respectively. Select 2 mL of PDA medium with medicine and spread it on the glass slide. After the medium on the glass slide is solidified, absorb a drop of Penicillium italicum spore suspension (concentration: 5×10 6 cfu / mL) was dropped on the medium slide, and then the slide was placed in a petri dish, repeated three times, and cultured at a constant temperature of 26°C for about 10h to observe the spore germination.

[0035] The minimum inhibitory concentrations of the three compound fungicides are shown in Table 2.

[0036] Table 2 Determination r...

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Abstract

The invention discloses an application of a carbendazim and galangin compound bactericide in citrus rot prevention and fresh keeping. The application comprises the following steps: (1) preparing a carbendazim mother solution: weighing carbendazim powder, and diluting the powder with sterile water to obtain the carbendazim mother solution; (2) preparing a galangin mother solution: weighing galangin, adding dimethyl sulfoxide for dissolution, and adding sterile water to a fixed volume to obtain the galangin mother solution; (3) preparing the carbendazim and galangin compound bactericide: mixingthe carbendazim mother solution with the galangin mother solution according to an equal volume proportion to obtain the carbendazim and galangin compound bactericide; and (4) applying the carbendazimand galangin compound bactericide on the surface of citrus. The bactericide is simple in method and convenient to use, can alleviate the problem of microbial drug resistance caused by long-term use ofa single drug, reduces the use dosage of current bactericides, and is greener and pollution-free. The bactericide effectively inhibits penicillium disease after pickling citrus, and prolongs the preservation period of picked citrus.

Description

technical field [0001] The present invention relates to the technical field of fruit preservation, and more specifically relates to the application of a compound fungicide of carbendazim and galangin in antiseptic preservation of citrus. The shelf life of the fruit. Background technique [0002] Citrus has a variety of varieties, rich nutrition, sweet and sour taste, and is deeply loved by people. At present, 90% of citrus in my country are mainly sold fresh, but citrus fruits are easily infected with green mold during storage and transportation, which leads to fruit rot and deterioration, seriously affecting product sales. Common antiseptic and fresh-keeping methods on the market are physical, chemical and biological control. In addition, there are some newly developed composite preservatives. Due to the limitations of physical control and biological control, the most widely used method in production is the use of chemical means. Commonly used chemical fungicides were fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
CPCA23B7/154
Inventor 彭丽桃程晨杨书珍
Owner HUAZHONG AGRI UNIV
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