Method for evaluating antioxidant, spot-fading and skin-brightening efficacy by using zebra fish model

Abstract

The invention belongs to the technical field of detection. The invention discloses a method for evaluating antioxidant, spot-fading and skin-brightening efficacy by using a zebra fish model. The method comprises the following steps: carrying out an in-vitro experiment on zebra fish within 3 days after fertilization, taking menadione as a molding agent and an oxidative stress reagent as a dye, andtaking an experimental endpoint as the fourth day after fertilization; collecting a fluorescence picture and a white light picture of zebra fish yolk sacs as data, using yolk sac fluorescence intensity, namely software parameter brightness sum (B), for evaluating an antioxidant efficacy, and using yolk sac pigment intensity, namely software parameter opacity sum (O), for evaluating freckle-fadingand skin-brightening efficacy. The method uses menadione for the first time to establish evaluation of antioxidant, spot-fading and skin-brightening efficacy with the zebra fish model; establishes ananimal model that can evaluate antioxidant, spot-fading and skin-brightening efficacy of both oral health food and applying cosmetics and conforms to a principle of substitution; has a short experimental period, completes efficacy evaluation in 1-2 days, and can screen formulas in batches.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for evaluating the effects of anti-oxidation and blemish-lightening and skin-brightening by using a zebrafish model. Background technique [0002] From 1986, the United Kingdom partially banned cosmetic animal testing, and today the European Union, Brazil, Norway, Turkey, South Korea, India, New Zealand and Israel and other countries and regions prohibit the use of animals to evaluate the efficacy of cosmetics. Cosmetic efficacy evaluation mainly uses in vitro experiments and juvenile zebrafish (zebrafish within 5 days after fertilization are considered acceptable because they do not need to eat) for alternative experiments. [0003] In Chinese skin care cosmetics, the whitening effect has attracted much attention. Whitening mechanisms are divided into the following seven categories: 1. Tyrosinase activity inhibitors; 2. Melanocyte cytotoxic agents; 3. Age...

AI Technical Summary

Problems solved by technology

[0007] 1. The existing detection methods show that the whitening evaluation methods focus on tyrosinase activity inhibitors, reducing agents and melanin metabolism agents, and there is no method that can be used to evaluate the efficacy of improving pigmentation disorders such as chloasma and age spots;

[0008] 2. In vitro testing lacks the process of absorption, distribution, metabolism and excretion, so it is impossible to accurately predict whether the test product that improves pigmentation disorders is really effective in the human body, nor can it predict the test products that have the effect of improving pigmentation disorders after metabolism in the body Taste;

[0009] 3. In vitro testing does not have the advantage of visualization, and the display form is not intuitive, which is not conducive to the promotion of cosmetics and health food;

[0010] 4. Animal models of pigment metabolism disorders that can be used for alternative experiments have not been established;

[0011] 5. The human experiment period is long, which is not conducive to the efficacy evaluation of large quantities of test products, especially not suitable for early formula screening

Images