Dental pulp stem cell preparation method

A technology of dental pulp stem cells and dental pulp, applied in cell dissociation methods, biochemical equipment and methods, animal cells, etc., can solve the problems of dental pulp stem cell death, low success rate, and large cell damage, and reduce cell damage and apoptosis, simple and convenient application, and the effect of ensuring survival rate
CN110468098APending Publication Date: 2019-11-19北京中瑞联合生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京中瑞联合生物科技有限公司
Publication Date
2019-11-19

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Abstract

The invention discloses a dental pulp stem cell preparation method. The dental pulp stem cell preparation method includes the following steps of: transporting dental pulp, wherein a deciduous tooth isplaced in a sampling bottle, the sampling bottle and a medical ice bag are placed back into a transportation box to be transported back to a laboratory; separating dental pulp stem cells, wherein tweezers are adopted to clamp the crown of the tooth, then, a syringe is used for sucking up normal saline to blow a dental pulp cavity inwards from a fracture surface of the root of the tooth to wash and loosen the dental pulp, and using a broach for taking out the dental pulp; and proliferating and culturing the dental pulp stem cells, wherein a complete culture medium is adopted, a dental pulp precipitate is resuspended and transferred to a 12-well microplate, one broach is used for fixing the dental pulp, barbs on another broach are used for stripping the dental pulp to make the dental pulp become fine pieces, the cells in the dental pulp are released, and then culture is conducted. According to the dental pulp stem cell preparation method, the broach is adopted to strip the agglomerate dental pulp to release the stem cells in the dental pulp, damage caused by using collagenase and the like on the dental pulp stem cells is avoided, damage on the dental pulp stem cells during digestioncan be eliminated, therefore, the extraction success rate of the dental pulp stem cells reaches 100%, and application is simple and convenient.
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Description

technical field

[0001] The invention relates to the technical field of cell life science, in particular to a method for preparing dental pulp stem cells. Background technique

[0002] Dental pulp stem cells (DPSC) are a type of mesenchymal stem cells that exist in dental pulp tissue. They are easy to obtain and can be obtained from replaced deciduous teeth without causing functional and health damage to the donor site. It has strong proliferative ability and possesses multiple properties of stem cells. Under different induction conditions, it can differentiate into osteoblasts, adipocytes, nerve cells, endothelial cells, etc. Studies have compared DPSCs with bone marrow mesenchymal stem cells and found that they have similar immunophenotypes, but dental pulp stem cells have higher clone formation and proliferation rates, and exhibit strong calcified tissue formation capabilities. In particular, DPSCs have low expression of MHC-II molecules, etc., which have low immunogenici...

Claims

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