Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity, enzymolysis preparation method and application thereof

A glucosidase and hemp seed technology, which is applied in the field of hemp seed meal polypeptide and its enzymatic hydrolysis preparation, can solve the problems of underutilization of hemp seed meal and waste of hemp seed resources, so as to improve the utilization of biological activity High efficiency, clear use, and high activity

Inactive Publication Date: 2014-11-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most hemp producing areas in my country use hemp seeds as oil, only for extracting hemp seed oil, while the hemp seed meal rich in protei

Method used

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  • Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity, enzymolysis preparation method and application thereof
  • Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity, enzymolysis preparation method and application thereof
  • Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity, enzymolysis preparation method and application thereof

Examples

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Example Embodiment

[0037] Example 1

[0038] Take 500g hemp seed meal dry powder, the ratio of dry powder to water is 5g:100mL, adjust the pH to 10, adjust the temperature to 50℃, stir evenly for 2h at a speed of 200r / min, take the supernatant, and adjust the pH to 4 , Obtain protein precipitation, adjust its pH to 6.5, and dry to prepare hemp seed meal protein. Add this protein to water to dissolve, the ratio of protein to water is 7g:100mL, control the pH at 8.0, then add Alcalase 2.4L protease (Novozymes, Denmark), the ratio of protein weight to the enzyme activity is 0.5:1000, reaction The temperature is 55°C, the stirring speed is 100r / min, and the reaction time is 3.5h. Boil for 10 minutes, pass the enzymatic hydrolysate through a macroporous adsorption resin exchange column (DA201-C), gradually increase the ethanol concentration from pure water, and perform gradient elution with 0, 25, 50, 75, and 100% ethanol at different concentrations. The flow rate is controlled at 1.0mL / min, the absor...

Example Embodiment

[0039] Example 2

[0040] Take 500g hemp seed meal dry powder, the ratio of dry powder to water is 10g:100mL, adjust the pH to 9.5, adjust the temperature to 40℃, stir evenly for 1.5h at a speed of 250r / min, take the supernatant, and adjust the pH to 4.5, the protein precipitate is finally obtained, the pH is adjusted to 7.0, and dried to obtain hemp seed meal protein. Add this protein to water to dissolve, the ratio of protein to water is 5g:100mL, control the pH at 8.5, then add Alcalase 2.4L protease (Novozymes, Denmark), the ratio of protein weight to the enzyme activity is 2:1000, reaction The temperature is 65°C, the stirring speed is 150r / min, and the reaction time is 4h. Boil for 10 minutes, pass the enzymatic hydrolysate through a macroporous adsorption resin exchange column (DA201-C), gradually increase the ethanol concentration from pure water, and perform gradient elution with 0, 25, 50, 75, and 100% ethanol at different concentrations. The flow rate is controlled at...

Example Embodiment

[0041] Example 3

[0042] Take 500g hemp seed meal dry powder, the ratio of dry powder to water is 15g:100mL, adjust the pH to 10, adjust the temperature to 55℃, and stir evenly at a speed of 100r / min for 1h, take the supernatant, and then adjust the pH to 4.0 Finally, the protein precipitate is obtained, the pH is adjusted to 6.8, and dried to obtain hemp seed meal protein. Add this protein to water to dissolve, the ratio of protein to water is 4g:100mL, control the pH at 8.5, then add Alcalase 2.4L protease (Novozymes, Denmark), the ratio of protein weight to enzyme activity is 0.1~5:1000 The reaction temperature is 50~75℃, the stirring speed is 200r / min, and the reaction time is 4h. Boil for 10 minutes, pass the enzymatic hydrolysate through a macroporous adsorption resin exchange column (DA201-C), gradually increase the ethanol concentration from pure water, and perform gradient elution with 0, 25, 50, 75, and 100% ethanol at different concentrations. The flow rate is contro...

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Abstract

The invention discloses an enzymolysis preparation method of Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity. The method includes: drying Cannabis sativa seed meal and crushing it into powder, adding water and stirring the mixture evenly, adjusting the pH to 8.0-10.5, adjusting the temperature to 30-80DEG C, performing stirring at a uniform speed for 0.5h-2h, conducting centrifugation and adjusting the pH of the supernatant to 3-6, thus obtaining protein precipitate; adding water to dissolve the protein, with the proportion being 2-7g:100mL, adjusting the pH to 7.0- 9.5, then adding alkaline protease, with the ratio of the protein weight to the enzyme activity being 0.1-5:1000, keeping the temperature at 50-75DEG C, and controlling the time at 1-4h; subjecting the enzymatic hydrolysate to separation and purification through macroporous adsorption resin and a Sephadex column, and carrying out collecting and drying, thus obtaining the alpha-glucosidase inhibiting active peptide powder. The Cannabis sativa seed meal polypeptide provided by the invention adds new sources for alpha-glucosidase inhibiting active substances, realizes full utilization of raw materials, and has the advantages of high product activity, convenient and feasible preparation process, and environmental protection, etc.

Description

technical field [0001] The invention relates to a biological peptide and its preparation method and application, in particular to a hemp seed meal polypeptide with the activity of inhibiting α-glucosidase and its enzymolysis preparation method and application. Background technique [0002] Hemp, also known as hemp, has played an important role in the development of human history and is an important raw material for making paper, clothing, ropes, etc. Hemp seed is an important by-product of hemp processing, and it is also a precious medicine and food homologous substance. It is rich in protein, lecithin, linolenic acid, vitamins, calcium, iron and other mineral elements necessary for human body. It has a good preventive effect on constipation, hyperlipidemia, high blood pressure, diabetes and gastrointestinal diseases. It is a "natural health product" for long-term consumption of centenarians in Bama County, the hometown of longevity. [0003] At present, most of the hemp pr...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16A23L1/29A23L33/00
Inventor 吴晖任尧梁凯
Owner SOUTH CHINA UNIV OF TECH
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