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Simple method for culturing haematococcus pluvialis to produce astaxanthin

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the field of cultivating Haematococcus pluvialls to produce astaxanthin, which can solve the problem that the annual output is difficult to exceed 20 tons, the composition of the medium is complicated, the algal cell adhesion and shading, etc. problems, to achieve the effect of easy expansion of production capacity, low cost, and cheap procurement

Active Publication Date: 2009-11-25
LIJIANG CHENGHAI BAOER BIOLOGICAL DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrogen content of the culture solution disclosed by the first method above is 5-20 mg / L, and the phosphorus content is 0.5-2 mg / L. The content of nitrogen and phosphorus in the culture medium is relatively high, and the composition of the culture medium is relatively complicated. Need to discharge wastewater containing nitrogen and phosphorus frequently
The above two types of methods must be carried out in a closed photobioreactor, the cost of producing astaxanthin is relatively high, and the production capacity of astaxanthin is small, and the annual output is difficult to exceed 20 tons
Photobioreactors are prone to cause algae cell adhesion and shading, resulting in a decrease in light transmittance during the cultivation period, and difficulties in cleaning the pipe wall.

Method used

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Examples

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Effect test

Embodiment 1

[0025]Culture solution preparation: select clean fresh water, filter, boil, then cool, add nitrogen and phosphorus-containing nutrient salts to prepare a culture solution with a nitrogen content of 6 mg / L and a phosphorus content of 0.2 mg / L for use. In the present embodiment, also can be in the culture fluid: the content of nitrogen is 2mg / L, and the content of phosphorus is 0.1mg / L; Or the content of nitrogen is 3mg / L, and the content of phosphorus is 0.1mg / L; Or the content of nitrogen is 4mg / L, the phosphorus content is 0.15mg / L; or the nitrogen content is 8mg / L, the phosphorus content is 0.3mg / L; the nitrogen content is 10mg / L, the phosphorus content is 0.4mg / L, etc.; but It is better to have a nitrogen-to-phosphorus weight ratio greater than 25.

Embodiment 2

[0027] The first step is cultivated: in the Erlenmeyer flask of 5000 milliliters, pack the nutrient solution of 3000 milliliters embodiment 1, in aseptic room inoculate Haematococcus pluvialls to the nutrient solution in the conical flask then, make every milliliter of nutrient solution Pluvialls The number of cells of Haematococcus is 1000-6000, and it is better to detect about 3000 / ml. Use soft light, control the light intensity on the surface of the Erlenmeyer flask in the range of 1500Lux-3000Lux, and control the culture temperature at 15°C-25°C. At this time, it is better to control the culture temperature at 16°C to 20°C, and the light hours are controlled at 10 hours to 18 hours for static cultivation. The light hours are preferably about 12 hours, and the cell density of the cultured Haematococcus pluvialis reaches 50,000 to 100,000 per milliliter to obtain primary vegetative growth. It is better to detect about 80,000 per milliliter in the primary vegetative growth, an...

Embodiment 3

[0031] It is basically the same as Example 2, except that in the second step of cultivation, nylon bags are used instead of plastic buckets, and in the third step of cultivation, halogen salts are used instead of mineral salts, and the concentration of halogen salts in the liquid in the pond is 0.3% to 0.5% by weight. %, H 2 o 2 The weight percent concentration is 0.002% to 0.005%, FeCl 2 The weight percent concentration of the nitrogen is 0.006% to 0.008%, and the weight ratio of nitrogen and phosphorus is about 0.1.

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Abstract

The invention discloses a simple method for culturing haematococcus pluvialis to produce astaxanthin; the method comprises three culturing processes of narrow neck flask, plastic cask or Nylon bag, cement pit; each step passes through various physical and chemical environmental parameters, such as nitrogen and phosphorus contents, pH value, incubation temperature, illumination intensity, illumination time, exact adjusting and controlling measure of adding with halite or halogeno salt, H2O2 and FeCl2 and cell state, etc; each step has definite object, and reasonable control such that the haematococcus pluvialis has excellent vegetative growth, fast conversion of vegetative growth to non-vegetative growth, and accumulates much astaxanthin, thereby improving the efficiency and stability of culturing, converting and accumulating, and effectively preventing the pollution of mixed algae; because the invention is an open culturing and does not need closed photobioreactor, thereby greatly reducing the production cost and obviously improving the productivity, and open culturing haematococcus pluvialis to produce astaxanthin in large scale.

Description

technical field [0001] The invention relates to a method for cultivating Haematococcus pluvialls to produce astaxanthin, in particular to a simple method for cultivating Haematococcus pluvialls to produce astaxanthin. Background technique [0002] Astaxanthin is a kind of carotenoid, mainly used as a natural colorant of fish. It has super anti-oxidation ability, protects the skin, improves immunity, inhibits cancer and other functions, and is widely used in aquatic products, cosmetics, medicine and other industries. Haematococcus pluvialis belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococcus pluvialis, and Haematococcus pluvialis. Haematococcus pluvialis is currently known as the organism with the highest astaxanthin content. It accumulates astaxanthin in a stressful environment, such as nutrient deficiency, strong light, etc., and its astaxanthin content can reach 1-4% of the cell mass , is an algae with great potential for large-scale production of astaxanthin. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/89
Inventor 骆其君严小军黄胜奎陈黎明王瑞陈苏苏
Owner LIJIANG CHENGHAI BAOER BIOLOGICAL DEV
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