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Enterobacter cloacae strain and applications thereof

A technology of Enterobacter cloacae and strains, applied in the field of microorganisms, to achieve the effects of fast growth, low mutation and good degradation effect

Inactive Publication Date: 2017-03-08
普洱学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of Enterobacter cloacae bacterial strain and its application, aim at solving the problem of degrading high-concentration metalaxyl pesticide residue, provide a kind of microbial strain that efficiently degrades high-concentration metalaxyl pesticide residue, this bacterium can Efficiently degrades high-concentration metalaxyl pesticide residues, and the bacteria can be used for the biodegradation of metalaxyl residues in water, soil and surfaces

Method used

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  • Enterobacter cloacae strain and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The cultivation of embodiment 1 thalline: use the inoculation needle to pick the mycelia of Enterobacter cloacae (Enterobacter cloacae) and inoculate it in the plate of the above-mentioned strain activation medium, and cultivate it in a 28°C incubator for 4 days at a temperature, and the bacterial strain is a stubby rod Shaped, 0.6μm~1.1μm×1.2μm~3.0μm, Gram stain negative, no spores, no capsule, with whole body flagella (6-8 flagella) positive motility, able to move, facultative anaerobic. Scrape the bacteria with a scalpel and inoculate them into a conical flask filled with 500ml of culture medium that was autoclaved at 121°C and 0.1MPa for 20min. Then place it at 30° C. with a rotation speed of 240 rpm / min and shake it on a shaking table for 4 days to obtain a mixed bacterial agent of mycelium and bacterial liquid.

Embodiment 2

[0026] Embodiment 2 Supplements the degradation of metalaxyl residues in the carbon source solid-state medium: when the strain activation medium is sterilized and cooled to 40° C., mixed with metalaxyl pesticide through ultraviolet sterilization for 30 minutes, the concentration is 5 mg / L, After mixing well, pour it into a petri dish with a diameter of 60ml, with 3mL medium per dish, and the culture medium is pink. The mycelia cultivated in Example 1 were punched into small pieces along the edge of the colony with a puncher with a diameter of 6 mm. After the medium mixed with metalaxyl pesticide is condensed, a small piece of mycelium is inserted, and the mycelium is close to the surface of the medium downward. Placed in a 30°C incubator for 10 days, the pink color in the medium has faded, the molecular structure of metalaxyl pesticide has been degraded, and the medium is creamy translucent.

Embodiment 3

[0027] Embodiment 3: degrading bacterial agent is to the degradation of high concentration metalaxyl pesticide residue

[0028](1) Add 5mg / L of metalaxyl pesticide to 100ml of the cultured culture medium, culture at 30°C with a rotation speed of 240rmp / min on a shaking table, and add metalaxyl of equal concentration to the uninoculated culture medium Ling pesticide was used as the control group;

[0029] (2) Sampling once every 12h, get 3ml samples and place them in 50mL of Enterobacter cloacae (Enterobactercloacae) to degrade the metalaxyl pesticide residues on the surface of water, soil and tobacco leaves. Shake on the shaker for 30 minutes, centrifuge at 3000rmp / min, take out the supernatant, repeat 3 times, combine the supernatant, extract the metalaxyl pesticide residues in the culture medium, and pass through Florisil in a solid phase extraction device Soil solid-phase extraction column (specification: 500mg3ml) purification, rotary evaporator concentration (below 40 ° ...

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Abstract

The invention discloses an enterobacter cloacae strain and applications of the enterobacter cloacae strain. The enterobacter cloacae strain is assigned with the accession number of CGMCC No.12819. The invention discloses the enterobacter cloacae which is obtained by separating the soil at the root of tobacco and can efficiently degrade the bactericide, namely, metalaxyl. The experiment proves that enterobacter cloacae can grow in an LB culture medium by taking metalaxyl as the unique carbon source and energy, and can degrade metalaxyl; under the liquid culture condition with added exogenous nutrients, enterobacter cloacae can degrade 90% or above of metalaxyl with the high concentration being 5mg / L mixed in the culture medium within 48h, and has good degrading effect within the wide pH range. With the degrading fungicide produced by adopting the technical scheme provided by the invention, the metalaxyl pesticide residue in the water body and soil and on the surfaces of tobacco leaves can be efficiently degraded, so that the ecological environment is protected and repaired, and the technical gap of degrading the metalaxyl pesticide residue by adopting enterobacter cloacae at present is made up.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an Enterobacter cloacae strain and application thereof. Background technique [0002] Chemical pesticides have the advantages of wide application range, many control objects, low production cost, good control effect and high economic benefit. Their wide application has greatly promoted the development of modern agriculture and improved the labor efficiency and mechanization degree of agricultural production. However, it is unavoidable that a large number of pesticides are used to kill insect natural enemies and beneficial microorganisms, make harmful organisms develop resistance, cause human and animal poisoning, and easily cause phytotoxicity to plants. Some of the chemical pesticides entering the environment can be exposed to sunlight, The action of microorganisms in the soil will gradually degrade and fail; the other part will eventually enter the human body...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C09K17/14A62D3/02B09C1/10C09K101/00A62D101/04C12R1/01C02F101/30
CPCA62D3/02A62D2101/04B09C1/10C02F3/34C02F2101/306C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 成文章张春花蒋智林单治国饶智王绍坤王超罗华元
Owner 普洱学院
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