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Phlebia acerina strain and application thereof in degrading metalaxyl pesticide residue

A technology of P. aceriformis and pesticide residues, applied in the field of environmental microorganisms, can solve the problems of phytotoxicity of plants, entry into the human body, and human hazards, and achieve the effects of not easy to mutate, fast growth, and simple cultivation methods

Active Publication Date: 2013-05-29
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is unavoidable that a large number of pesticides are used to kill insect natural enemies and beneficial microorganisms, make harmful organisms develop resistance, cause human and animal poisoning, and easily cause phytotoxicity to plants. Some of the chemical pesticides entering the environment can be exposed to sunlight, The action of microorganisms in the soil, etc., gradually degrades and fails; the other part will eventually enter the human body through the action of the "food chain", causing long-term harm to humans
At present, there is no literature report on the use of Phlebia acerina to degrade metalaxyl pesticide residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The culture of embodiment 1 thalline: pick the mycelia of Phlebia acerinaY3667 with an inoculation needle and inoculate it into the above-mentioned bacterial species activation medium plate, and cultivate it in a 28°C incubator for 4 days, and the colony is loose and snowy. White, cottony. Scrape the loose hyphae with a scalpel and inoculate them into a conical flask filled with 500ml of culture medium that was autoclaved at 121°C and 0.1MPa for 20min. Then place it at 30° C., rotate at 140 rpm / min, and culture on a shaking table for 4 days to obtain a mixed bacterial agent of mycelium and bacterial liquid.

Embodiment 2

[0029] Example 2 Degradation of metalaxyl residues in supplemented carbon source solid medium: when the strain activation medium was sterilized and cooled to 40°C, mixed with metalaxyl pesticide sterilized by ultraviolet light for 30 minutes, the concentration was 5mg / L, After mixing well, pour it into a petri dish with a diameter of 60 mm, with 3 mL of culture medium per dish, and the culture medium is pink. The mycelia cultivated in Example 1 were punched into small pieces along the edge of the colony with a puncher with a diameter of 6 mm. After the medium mixed with metalaxyl pesticide is condensed, a small piece of mycelium is inserted, and the mycelium is close to the surface of the medium downward. Placed in a 30°C incubator for 10 days, the pink color in the medium has faded, the molecular structure of metalaxyl pesticide has been degraded, and the medium is creamy translucent.

Embodiment 3

[0030] Example 3: Degradation of high-concentration metalaxyl pesticide residues by degrading bacteria agent

[0031] (1) Add 5mg / L of metalaxyl pesticide to 100ml of the culture medium cultivated in claim 3, and culture it on a shaking table at 30°C with a rotation speed of 140rmp / min, and add an equal concentration to the uninoculated culture medium The metalaxyl pesticide was used as the control group;

[0032](2) Take a sample every 12 hours, take 3ml of the sample and place it in a 50mL plastic centrifuge tube, add 10mL of acetonitrile, shake on the shaker for 30min, centrifuge at 3000rmp / min, take out the supernatant, repeat 3 times, and combine the supernatant , after extracting the metalaxyl pesticide residues in the culture solution, it was purified by a Florisil solid-phase extraction column (specification: 500mg3ml) in a solid-phase extraction device, concentrated by a rotary evaporator (below 40°C), and stored at -20°C After freeze-drying, the extracted pesticide ...

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Abstract

The invention relates to phlebia acerina strain and application thereof in degrading metalaxyl pesticide residue, and belongs to the biological technical field of organisms. The strain is utilized for degrading high-concentration metalaxyl pesticide residue. According to the identification, the scientific name of the white-rot fungi is phlebia acerina Y3667 which is stored in the Common Organism Center of the China Committee for Culture Collection of Microorganisms, wherein the storage number is CGMCC 6809; the ITS (Internal Transcribed Spacers) sequences are extracted by the DNA, cloned and measured; compared with the stored sequences in GenBank, the homology of the phlebia acerina strain with the phlebia acerina strain BMC3014 is 99%. The strain is cultivated in liquid medium to obtain microbial inoculum; and the microbial inoculum is added with the high-concentration metalaxyl pesticide for effectively degrading the metalaxyl pesticide residue. The degrading microbial inoculum generated by the technical scheme in the invention can be used for effectively degreasing the metalaxyl pesticide on the surface of water and agricultural products, so that the ecological environment is protected and restored.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and uses microorganisms to degrade chemical pesticide residues, and relates to a strain of Phlebia acerina and its application in the degradation of high-concentration metalaxyl pesticide residues. Background technique [0002] Chemical pesticides have the advantages of wide application range, many control objects, low production cost, good control effect and high economic benefit. Their wide application has greatly promoted the development of modern agriculture and improved the labor efficiency and mechanization degree of agricultural production. However, it is unavoidable that a large number of pesticides are used to kill insect natural enemies and beneficial microorganisms, make harmful organisms develop resistance, cause human and animal poisoning, and easily cause phytotoxicity to plants. Some of the chemical pesticides entering the environment can be exposed to sunlight, The acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34A62D3/02C12R1/645C02F101/38A62D101/04A62D101/28
Inventor 袁海生戴玉成魏玉莲
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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