Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Short term storage method for large-size fungus species

A technology for strains and fungi, applied in fungi and other directions, can solve the problems of strains easily mutated, strains inactivated, time-consuming and space-consuming, and achieve the effect of not easy to mutate.

Inactive Publication Date: 2007-04-25
SOUTH CHINA AGRI UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this preservation method are (1) time-consuming and space-consuming; (2) the medium is easy to lose water and inactivate the bacteria; (3) the tampon is easy to grow mold to contaminate the bacteria; (4) the number of transfers is too many Strains prone to mutation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Short term storage method for large-size fungus species
  • Short term storage method for large-size fungus species
  • Short term storage method for large-size fungus species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the preservation of Flammulina velutipes bacterial classification

[0019] 1. The medium used is the commonly used Flammulina velutipes culture medium, and their formulas are respectively:

[0020] PDSA solid plate medium: peeled potato 200g, glucose 20g, KH 2 PO 4 3g, MgSO 4 1.5g, vitamin B 1 5-10mg, agar 20g, H 2 O 1L;

[0021] 2. The tested Flammulina velutipes strains were purchased from the Edible Fungi Research Center of Xijiu Town, Xishui County, Guizhou City, and the strain numbers were Fve1, Fve2, Fve3, Fve4, and Fve5.

[0022] Specific steps are as follows:

[0023] (1) Prepare solid plate medium according to conventional methods, and inoculate 0.5cm 2 Five large and small slanted female species of Flammulina velutipes were placed in the middle of the plate, and cultured at a temperature of 21°C after inoculation;

[0024] (2) One week later, when the mycelium has just overgrown the petri dish, use a scalpel to gently divide the plate i...

Embodiment 2

[0026] Embodiment 2: the detection of Flammulina velutipes preserved bacterial classification

[0027] 1. Inclined plate mycelium experiment:

[0028] (1) Prepare the PDSA medium according to the routine, and make it into a flat plate and a slant.

[0029] (2) Take out the Flammulina velutipes bacteria strains preserved in Example 1 from the refrigerator at 4° C. every other month, inoculate them on slopes respectively, and then place them in a constant temperature incubator at 21° C. for cultivation.

[0030] (3) Observed after one week, it was found that the mycelia of the strains preserved for 1 to 10 months had normal mycelium morphology and good growth status (see Figure 1 and Figure 2), and the strains' viability decreased after 11 months of preservation.

[0031] (4) In the ultra-clean workbench, the strains preserved at 4°C were connected to the plate, and no antagonistic lines were produced when the mycelium of the colonies came into contact, indicating that the stra...

Embodiment 3

[0044] The preservation method of the present invention is compared with the existing slope preservation method, see Table 1 for details.

[0045] Table 1 The inventive method compares with inclined-plane cryopreservation method

[0046] method

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention disclosed a way of preserving the big fungus strain for a short term. It comprises the following steps: (1) inoculating the strain to the solid medium and cutting the medium into several pieces when the hyphal body nearly covered the whole flat plate; (2) adding sterile distilled water to the container first, then putting the pieces of medium in the container; covering the lid and preserving it at 4 Deg C. The invention can preserve the strain for a short term without variation; besides, it's very simple and economical.

Description

technical field [0001] The invention relates to the technical field of strain preservation methods, in particular to a short-term preservation method for macrofungal strains. Background technique [0002] At present, the short-term preservation method of macrofungal strains is mainly inclined-plane cryopreservation method, that is, the strains to be preserved are inoculated on a suitable inclined-plane medium, cultivated at a suitable temperature, and taken out when the mycelium grows robustly on the inclined-plane, and placed on 3 Store in a low-temperature dry place at -5°C or in a refrigerator or freezer at 4°C, and transplant and transfer tubes every 4-6 months. The disadvantages of this preservation method are (1) time-consuming and space-consuming; (2) the medium is easy to lose water and inactivate the bacteria; (3) the tampon is easy to grow mold to contaminate the bacteria; (4) the number of transfers is too many Strains are prone to variation. (Yang Xinmei, "Edib...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 林俊芳郭丽琼王秀旭阮秋菊韩君莉
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com