This application discloses a method for detecting CHI3L1
protein based on microfluidic
fiber optic sensing, belonging to the field of biomedical detection technology. The method includes: providing a microstructured
fiber optic sensing unit with a through-flow
microfluidic channel; immobilizing a CHI3L1
protein-
specific antibody on the inner surface of the
microfluidic channel; injecting the sample to be tested into the channel, causing the CHI3L1
protein to be specifically captured; and achieving quantitative detection of the CHI3L1 protein by detecting the drift of
fiber optic transmission spectral characteristic parameters caused by
antigen-
antibody binding. This invention combines
microfluidics with fiber optic sensing, utilizing
antibody immobilization on the inner wall of the
microfluidic channel to increase the interaction efficiency between the
light field and biomolecules, significantly improving detection sensitivity. It has the advantages of
rapid detection, low sample consumption, and
label-free operation, making it suitable for early non-invasive screening and
dynamic monitoring of clinical
liver fibrosis.