Construction method and application of caenorhabditis elegans hyperuricemia model and screening method of construction method

Abstract

The invention relates to a construction method of a caenorhabditis elegans hyperuricemia model. The method comprises the following steps that 1, the uric acid content in caenorhabditis elegans is measured through a high performance liquid chromatography method; 2, an optimal drug delivery and culture mode is determined; 3, an optical drug delivery substance and drug delivery dosage are determined; 4, the optimal drug delivery time of xanthine is determined; 5, series experiment evaluation is conducted on the caenorhabditis elegans hyperuricemia model obtained through the construction method, and the caenorhabditis elegans hyperuricemia model which is low in damage, efficient, stable and reliable is obtained. According to the construction method, the hyperuricemia animal model is constructed by means of the model organism caenorhabditis elegans which has the high conservative property to human genes and has the similar uric acid metabolic pathway with human beings, the model has typical pathological characterization and is stable and reliable, various quantity indexes have the statistical significance, and a powerful tool is provided for large-scale and high-throughput drug screening of gout and the hyperuricemia symptom.

Description

technical field [0001] The invention belongs to the technical field of animal models of human chronic diseases, and relates to a method for establishing an animal model of human chronic diseases, in particular to a construction method and application of a Caenorhabditis elegans hyperuricemia model and a screening method for the construction method. Background technique [0002] With the improvement of people's living standards and changes in dietary structure, the incidence of hyperuricemia has been significantly increasing worldwide in recent years. Hyperuricemia itself not only poses a great threat to human health, but also produces various complications, such as cardiovascular and cerebrovascular diseases and atherosclerosis. Therefore, it has become an urgent task to strengthen the prevention and control of hyperuricemia and the research and development of related drugs. However, the lack of existing animal models of hyperuricemia has become a major obstacle in the path...

AI Technical Summary

Problems solved by technology

Different drug administration methods have their own advantages and disadvantages: the uneven food intake of feeding animals will make the blood uric acid level in the body unstable, but the duration is longer; intragastric administration can avoid the unstable blood uric acid level; injection is easy to operate, but The hyperuricemia state lasts for a short time, so it is not suitable for the study of pathogenesis; the combined administration of several methods is often effective, and the constructed hyperuricemia model has the characteristics of persistence and stability; the model mice established by gene knockout survived Short time, high cost

[0009] Through searching, there is no relevant report on the establishment of hyperuricemia model using Caenorhabditis elegans

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