Application of Pseudomonas aeruginosa polyclonal antibody during salmonella separation process

A polyclonal antibody, Pseudomonas aeruginosa technology, applied in the field of Salmonella separation and identification, can solve problems such as loss of identification work

Inactive Publication Date: 2012-10-17
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These interfering bacteria bring unnecessa

Method used

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Embodiment Construction

[0004] (1) Preparation and purification of Pseudomonas aeruginosa antibody

[0005] Pseudomonas aeruginosa was inoculated on nutrient agar, and after 12 hours at 37°C, the bacterial lawn was collected and dissolved in 0.85% NaCl. Prepare 2 mg / mL bacterial solution, add an equal volume of IFA (incomplete Freund's adjuvant) to emulsify, and then immunize New Zealand white rabbits with an antigen dose of 1 mg. Rabbits were immunized with the same dose 2 weeks later, and then boosted immunized 2 weeks later, the dose was half of the initial immunization. Blood was collected 1 week after booster immunization, and antibodies were purified by saturated ammonium sulfate method.

[0006] (2) Addition of antibody

[0007] Add an appropriate amount of antibody to the enrichment solution of Salmonella, and isolate Salmonella by conventional methods after 12 hours at 37°C.

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Abstract

The invention relates to an application of a Pseudomonas aeruginosa polyclonal antibody during a salmonella separation process. Acceding to the invention, the polyclonal antibody is used for detecting salmonella, the method comprises the following steps: preparing the polyclonal antibody of interference bacteria-Pseudomonas aeruginosa during the salmonella separation process, purifying by a saturation ammonium sulfate method, adding the purified antibody in a selective enrichment broth during the salmonella separation process, and reacting for 12 hours at the temperature of 37 DEG C and then carrying out subsequent separating culture.

Description

technical field [0001] The invention belongs to the scope of isolation and identification of Salmonella. Background technique [0002] Salmonella is one of the important enteropathogenic bacteria in current public health, and it is also one of the pathogenic bacteria causing intestinal infectious diseases in animals. At present, the detection methods for Salmonella mostly use conventional bacterial isolation and identification, that is, the steps of non-selective enrichment, selective enrichment, isolation and identification are firstly adopted. Interfering bacteria usually appear during the isolation process, Pseudomonas aeruginosa is one of them, and the probability of occurrence is relatively high. These interfering bacteria brought unnecessary losses to the subsequent identification work. The invention patent is to eliminate the interference caused by Pseudomonas aeruginosa by adding the polyclonal antibody of Pseudomonas aeruginosa to the selective enrichment solution...

Claims

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Application Information

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IPC IPC(8): C12N1/20C07K16/12C07K16/06
Inventor 肖西志吴振兴高宏伟邓明俊郑小龙王群孙涛朱来华岳志芹臧伟卢瑜孙敏林超徐彪梁成珠
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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