A method for enriching Danshensu in fermented liquid by using macroporous adsorption resin

A technology of pore adsorption and danshensu, which is applied in the field of separation and purification of fermentation products, can solve the problems that the method of danshensu has not been published in the newspapers, etc., and achieve the effect of good industrial application prospect, simple operation and high yield

Inactive Publication Date: 2016-08-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of separating and purifying Danshensu from E. coli fermentation broth has not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for enriching Danshensu in fermentation broth using macroporous adsorption resin, comprising the following steps:

[0027] (1) Centrifuge the fermentation broth obtained by fermenting Danshensu-producing Escherichia coli to obtain a supernatant, adjust the pH to 2.0, and use it as a sample solution for subsequent use;

[0028] (2) Accurately weigh 50g each of the pretreated macroporous adsorption resins X-5 and ADS-17, and respectively wet-pack them into columns (25*300 with sand cores) with a sample flow rate of 2BV / h. (1) The obtained sample solution is added to the macroporous adsorption resin column;

[0029] (3) Adding 50 mL of aqueous ethanol solution with a volume concentration of 80% to the macroporous adsorption resin column respectively, eluting through the macroporous adsorption resin column at a flow rate of 1 BV / h, collecting 10 to 40 mL of this eluate;

[0030] (4) Concentrate and dry the eluate to obtain Danshensu.

[0031] Calculate the adsorp...

Embodiment 2

[0035] A method for enriching Danshensu in fermentation broth using macroporous adsorption resin, comprising the following steps:

[0036] (1) Centrifuge the fermented liquid obtained by Escherichia coli producing Danshensu to obtain the supernatant, and take 3 parts to adjust the pH=1.5, pH=2.0, and pH=3.0 respectively, and use it as a sample solution for later use;

[0037] (2) Accurately weigh 3 parts of pretreated model X-5 macroporous adsorption resin, 50g each, respectively wet packing (25*300 with sand core) and 250mL pH=1.5, pH=2.0, pH=3.0 The sample solution is added to the X-5 macroporous adsorption resin column at a sample flow rate of 2BV / h;

[0038] (3) Adding 50 mL of aqueous ethanol solution with a volume concentration of 80% to the macroporous adsorption resin column respectively, eluting through the macroporous adsorption resin column at a flow rate of 1 BV / h, collecting 10 to 40 mL of this eluate;

[0039] (4) Concentrate and dry the eluate to obtain Danshen...

Embodiment 3

[0042] A method for enriching Danshensu in fermentation broth using macroporous adsorption resin, comprising the following steps:

[0043] (1) Centrifuge the fermentation broth obtained by fermenting Danshensu-producing Escherichia coli to obtain a supernatant, adjust the pH to 2.0, and use it as a sample solution for subsequent use;

[0044] (2) Accurately weigh 3 parts of pretreated X-5 macroporous adsorption resin, 50g each, and wet-pack the column (25*300 with sand core) respectively, and take 150mL, 200mL, 250mL of the sample solution, and the sample flow rate Add 2BV / h to the X-5 macroporous adsorption resin column;

[0045] (3) Adding 50 mL of aqueous ethanol solution with a volume concentration of 80% to the macroporous adsorption resin column respectively, eluting through the macroporous adsorption resin column at a flow rate of 1 BV / h, collecting 10 to 40 mL of this eluate;

[0046] (4) Concentrate and dry the eluate to obtain Danshensu.

[0047] The calculated ads...

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Abstract

The invention discloses a method for enriching tanshinol in fermentation broth by using macroporous resin. The method comprises the following steps: (1) centrifuging fermentation broth obtained by fermenting Escherichia coli SyBE 002444 with a preservation number of CGMCC No.7961 to obtain a supernatant, and regulating the pH to 1.5-3.0 to serve as a liquid sample for later use; (2) adding the liquid sample into a macroporous resin column; (3) adding an ethanol aqueous solution with volume concentration of 70-90% into the macroporous resin column, eluting the liquid sample at a flow rate of 0.5-2 BV / H through the macroporous resin column, and collecting eluant; (4) concentrating and drying the eluant to obtain tanshinol. The method disclosed by the invention has the advantages of simplicity in operation, high yield, environmental friendliness and the like. The method disclosed by the invention can be applied to separation and purification of tanshinol production by microbiological fermentation.

Description

technical field [0001] The invention relates to the separation and purification of fermentation products, in particular to a method for enriching danshensu in fermentation liquid by using macroporous adsorption resin. Background technique [0002] Macroporous adsorption resin is a kind of organic polymer copolymer with macroporous structure, and it is a kind of artificially synthesized organic polymer adsorbent. Macroporous adsorption resin adsorption technology was first used in wastewater treatment, pharmaceutical industry, chemical industry, analytical chemistry, clinical testing and treatment, etc. In recent years, it has been widely used in the extraction, separation and purification of active ingredients of Chinese herbal medicines, such as flavonoids, saponins , Refining and purification of traditional Chinese medicine ingredients such as alkaloids. [0003] Danshensu (Salvianic acid A, Danshensu), belongs to acidic aromatic compounds, its chemical name is β-(3,4-dih...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/52C07C51/47
CPCC07C51/47C07C59/52
Inventor 赵广荣白晨龙
Owner TIANJIN UNIV
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