Staphylococcus aureus separation method for eliminating interference of food background miscellaneous bacteria
A technology of staphylococcus and separation method, applied in the field of staphylococcus aureus separation, can solve the problems of hemolysis, misjudgment, difficult separation of staphylococcus aureus and the like
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Embodiment 1
[0029] A method for isolating Staphylococcus aureus that can eliminate the interference of background bacteria, comprising the following steps:
[0030] Step 1, strain activation and preparation of working bacterial solution:
[0031] Streak the strains stored in the glycerol tubes in the -80°C ultra-low temperature refrigerator to activate them on ordinary trypsin soybean agar plates, culture them statically at 37°C for 18-24 hours, and then store them in the refrigerator at 4°C. Pick a single colony and inoculate it into TSB, and place it in a shaker incubator at 37°C and 200 rpm for overnight culture. After the culture, centrifuge at 10000rpm for 30s, discard the supernatant, wash the precipitate with saline containing 0.85% sodium chloride, repeat twice, and finally adjust the OD with a microplate reader 600 Prepared as a working bacterial solution.
[0032] Step 2, determine the inoculation ratio:
[0033] Adjust ATCC 29213 and miscellaneous bacteria to a consistent OD...
Embodiment 2
[0055] The application of the Staphylococcus aureus enrichment and isolation method that can eliminate background miscellaneous bacteria interference includes the following steps:
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