Staphylococcus aureus separation method for eliminating interference of food background miscellaneous bacteria

A technology of staphylococcus and separation method, applied in the field of staphylococcus aureus separation, can solve the problems of hemolysis, misjudgment, difficult separation of staphylococcus aureus and the like

Active Publication Date: 2020-10-20
SHANGHAI JIAO TONG UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when Staphylococcus aureus was isolated from frozen meat and meat products (especially frozen chicken) in the early stage, it was found that due to the presence of diffuse background bacteria contamination, the selective plate was often covered with a layer of gray matter, which made the appearance of the colony The morphology changes, which causes misjudgment ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Staphylococcus aureus separation method for eliminating interference of food background miscellaneous bacteria
  • Staphylococcus aureus separation method for eliminating interference of food background miscellaneous bacteria
  • Staphylococcus aureus separation method for eliminating interference of food background miscellaneous bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for isolating Staphylococcus aureus that can eliminate the interference of background bacteria, comprising the following steps:

[0030] Step 1, strain activation and preparation of working bacterial solution:

[0031] Streak the strains stored in the glycerol tubes in the -80°C ultra-low temperature refrigerator to activate them on ordinary trypsin soybean agar plates, culture them statically at 37°C for 18-24 hours, and then store them in the refrigerator at 4°C. Pick a single colony and inoculate it into TSB, and place it in a shaker incubator at 37°C and 200 rpm for overnight culture. After the culture, centrifuge at 10000rpm for 30s, discard the supernatant, wash the precipitate with saline containing 0.85% sodium chloride, repeat twice, and finally adjust the OD with a microplate reader 600 Prepared as a working bacterial solution.

[0032] Step 2, determine the inoculation ratio:

[0033] Adjust ATCC 29213 and miscellaneous bacteria to a consistent OD...

Embodiment 2

[0055] The application of the Staphylococcus aureus enrichment and isolation method that can eliminate background miscellaneous bacteria interference includes the following steps:

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

In a first aspect, the invention discloses a Staphylococcus aureus separation method for eliminating the interference of food background miscellaneous bacteria. The separation method comprises an enrichment method and a purification method, the enrichment method uses an improved enrichment broth, and the purification method uses an improved trypsin soybean agar medium; in a second aspect, the invention further discloses an application of the Staphylococcus aureus enrichment method and purification method in the separation of Staphylococcus aureus; and in a third aspect, the invention also discloses a Staphylococcus aureus separation kit prepared by the application of the separation of Staphylococcus aureus. The Staphylococcus aureus separation method for eliminating the interference of food background miscellaneous bacteria provided by the invention effectively eliminates the interference of the miscellaneous bacteria, improves a separation rate of Staphylococcus aureus in food, has the advantages such as low detection method cost, simple operation, and high accuracy, and has broad application prospects and economic benefits.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for isolating Staphylococcus aureus for eliminating the interference of food background bacteria. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is an important food-borne pathogen, which often causes food poisoning, hospital and community-acquired infections, and is the key object of food-borne disease surveillance in my country. Because Staphylococcus aureus exists widely in nature and is zoonotic, it generally has a strong ability to form a bacterial film. Therefore, Staphylococcus aureus is considered to have the potential to spread drug resistance, and multi-drug resistance occurs from time to time. In recent years, as the problem of bacterial drug resistance has become increasingly serious, researchers have realized that multidrug-resistant Staphylococcus aureus contaminated in food may become a carrier of drug resistance ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/02C12R1/445
CPCC12N1/20C12N1/02Y02A40/90
Inventor 施春雷区楚君尚岱麒常江杨婧娴陈博
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap