Method for monitoring salmonella

Abstract

The invention discloses a method for monitoring salmonella. The method for monitoring salmonella comprises the following steps that an appropriate amount of a sample to be detected is mixed with a BWPsolution, the mixture is ground / stirred to obtain a suspension / mixed solution, insoluble matter is removed by centrifugation, then the bacteria is washed with normal saline, lytic cells are boiled, asupernatant is taken through centrifugation, the supernatant is placed in a sterile test tube, enrichment culturing is performed in an incubator for 8-10 hours at 36 DEG C, a BPW liquid culture is inoculated into selective enrichment broth SC of the salmonella, an enrichment culturing solution is inoculated on a SS medium in a one-ring mode, and medium-sized, circular colonies with black centerson the SS medium are selected for pure culture, and the purely cultured colonies are simultaneously subjected to dual PCR and biochemical identification. The detection time of the salmonella is shortened, the detection process is accelerated, the operation complexity is reduced, the labor intensity is reduced, the detection cost is reduced, and fast, simple, and sensitive detection of a target object is achieved, the dual PCR and biochemical identification are simultaneously carried out, so that the accuracy is improved.

Description

Technical field [0001] The present invention relates to the technical field of salmonella monitoring, in particular to a method for monitoring salmonella. Background technique [0002] Salmonella is a common food-borne pathogen, a gram-negative, intracellular parasitic intestinal bacteria. The bacterium is widespread in nature, and it can not only cause acute, chronic or invisible infections of livestock, poultry and other animals, but also cause food poisoning of humans by contaminating food, posing a great threat to humans. According to statistics, among the types of bacterial food poisoning in various countries in the world, food poisoning caused by Salmonella often tops the list. Salmonella causes about 40,000 Americans to fall ill each year and about 600 people die. Salmonella is the most common pathogenic bacteria in food poisoning in my country, and it ranks first in food poisoning. The clinical symptoms of salmonellosis mainly include headache, abdominal pain, fever, e...

AI Technical Summary

Problems solved by technology

At present, the detection of Salmonella mostly adopts the traditional microbial culture method, which mainly includes steps such as pre-enrichment, selective enrichment, plate separation, biochemical test and serological identification, which involves many experiments, cumbersome operations, and a long detection cycle. (Generally it takes 4-7 days), the preparation and finishing work are heavy, and it can no longer meet the realistic needs of rapid detection of pathogenic bacteria in food at this stage