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Pseudomonas aeruginosa DGNK-JL2 and application thereof

A DGNK-JL2, Pseudomonas aeruginosa technology, applied in the field of agricultural biology, can solve problems such as unfavorable agricultural sustainable development, depletion of phosphorus and phosphorus resources, and environmental pollution

Active Publication Date: 2019-12-27
DONGGUAN AGRI SCI RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the seasonal utilization rate of phosphorus fertilizers in China is only 5% to 25%, resulting in a large amount of phosphorus fertilizer residues in the soil being absorbed by soil minerals into insoluble phosphate, which cannot be absorbed and utilized by plants (Gustafsson et al., 2012; Roberts et al.,2015)
Long-term application of chemical phosphate fertilizers is also likely to cause problems such as depletion of phosphorus and phosphorus resources, environmental pollution, and soil compaction (Qin Lijin et al., 2006), which is not conducive to the sustainable development of agriculture
Therefore, how to transform the ineffective phosphorus in the soil and increase the content of available phosphorus in the soil is an effective way to solve the lack of phosphorus in the soil

Method used

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  • Pseudomonas aeruginosa DGNK-JL2 and application thereof
  • Pseudomonas aeruginosa DGNK-JL2 and application thereof
  • Pseudomonas aeruginosa DGNK-JL2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The separation of embodiment 1 decomposing organophosphate bacteria

[0034] After 2 months of composting the traditional Chinese medicine dregs, take the traditional Chinese medicine dregs from the composting place, use the three-point sampling method, mix them into 1 part, seal them in a sterile bag, and bring them back to the laboratory to decompose organophosphate bacteria.

[0035] Under sterile conditions, weigh 10g of medicinal dregs and dissolve in 90mL of sterile water to fully shake and gradiently dilute to 10 －3 、10 －4 、10 －5 times the drug residue suspension, and then take 100 μL of each dilution and evenly spread it on the organophosphate-dissolving bacterial medium plate, and incubate it upside down at 37°C for 3 days, and observe whether there is a phosphorus-dissolving circle. The isolates with phosphate-dissolving effect were streaked and purified on the plate, repeated 3 times, and the purity of the colonies was observed with a microscope until a pur...

Embodiment 2

[0036] The double screening of embodiment 2 solution organophosphate bacteria

[0037] Using the phosphorus-dissolving circle method, the bacterial strain to be tested obtained in Example 1 was spot-connected on the organic phosphorus solid medium, repeated 3 times, and cultivated in a 36°C incubator for 3 days, according to the diameter of the transparent circle (D) and the diameter of the colony (d). The ratio (D / d) preliminarily determines the strength of the strain's ability to decompose organic phosphorus.

Embodiment 3

[0038] The mensuration of embodiment 3 phosphorus dissolving ability

[0039] The strains screened in Example 2 were inoculated into 20 mL of high-temperature sterilized nutrient broth medium and activated at 36° C. and 150 r / min for 1 d as seed liquid. Use a pipette gun to draw the seed solution according to the inoculum amount of 2% and add it to 100mL of high-temperature sterilized Montina lecithin medium, and use the fermentation medium without any bacterial solution as the blank control group, and set 3 replicates for each treatment , and cultivated at 36°C and 150r / min for 5 days. The fermentation broth was centrifuged at 4°C, 8000r / min for 15 minutes, and the supernatant was taken, and the effective phosphorus content in the fermentation broth was determined by the molybdenum-antimony anti-colorimetric method. The difference between the soluble phosphorus content of the experimental group and the control group was the strain to be tested. Phosphorus-dissolving capacity...

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Abstract

The invention discloses pseudomonas aeruginosa DGNK-JL2. The pseudomonas aeruginosa DGNK-JL2 is collected by a Guangdong Microbial Culture Collection Center, which is located at fifth floor of building 59# of yard 100#, middle Xianlie road, Guangzhou, Guangdong 510075, on Aug 22, 2019 and has the collection number of GDMCC NO: 60748. A strain of phosphate solubilizing bacteria, i.e., the pseudomonas aeruginosa DGNK-JL2 is separated and screened out from traditional Chinese medicine decoction dreg compost and is subjected to identification and biological control action tests, so that a strain for preparing efficient biofertilizers is reserved, and the sustainable development of ecological agriculture is boosted; and by employing the pseudomonas aeruginosa DGNK-JL2 disclosed by the invention, the content of available phosphorus in soil can be increased by 8.6 times to the maximum, and fermentation broth of the strain DGNK-JL2 disclosed by the invention has a certain antagonistic action on pepper anthracnose and bitter melon blight.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a strain of Pseudomonas aeruginosa (Pseudomonasaeruginosa) DGNK-JL2 and its application. Background technique [0002] In order to efficiently utilize the "phosphorus pool" resources in the soil, reduce the amount of phosphate fertilizer used, alleviate the non-point source of agricultural pollution caused by excessive use of phosphate fertilizer, and improve the soil. In this experiment, the compost of traditional Chinese medicine dregs was used as the material, and the bacteria with phosphorus-dissolving ability were screened with organic phosphorus bacterial culture medium. Morphological observation, physiology and biochemistry and 16S rDNA identification were carried out on the bacteria; the antagonism of phosphate-solubilizing bacteria on rice sheath blight, pepper anthracnose and bitter gourd wilt was determined by plate confrontation method. [0003] Phosphorus i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P3/00C12R1/385
CPCC05F11/08A01N63/00C12R2001/385C12N1/205Y02A50/30
Inventor 胡珊梁卫驱黄皓陈仕丽罗华建徐匆
Owner DONGGUAN AGRI SCI RES CENT
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