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Pseudomonas aeruginosa and application

A Pseudomonas aeruginosa, denitrification technology, applied in the field of environmental engineering and biology, can solve problems such as affecting the yield and quality of agricultural products, wasting resources, and restricting economic development.

Active Publication Date: 2015-09-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, it affects the output and quality of agricultural products, on the other hand, it also restricts economic development and seriously endangers human health.
Not only caused serious damage to the environment, but also caused a lot of waste of resources

Method used

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  • Pseudomonas aeruginosa and application
  • Pseudomonas aeruginosa and application
  • Pseudomonas aeruginosa and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Isolation, purification and identification of strains

[0035] In this study, a piece of filler with biofilm was taken from the reactor of the above-mentioned bio-trickling filtration system into a conical flask containing 90 mL of sterile denitrification culture solution, and the biofilm on the filler was shaken in an oscillator until it fell off. After removing the filler, continue to shake the biofilm to break it up. The broken biofilm was re-extracted and added to a new sterile denitrification liquid medium, and placed in a 30°C, 160r / min air bath constant temperature shaker for shaking culture. After 24 hours, the inoculum with a volume ratio of 10% was inserted into 100 mL of fresh sterile denitrification culture solution. After repeating this process for three days, it was found that the bacterial solution grew well, and the denitrification rate reached more than 80%. Take 1ml of the bacterial solution into a test tube filled with 9ml sterile wate...

Embodiment 2

[0038] Example 2: Denitrification Performance Detection of Pseudomonas aeruginosa CP1

[0039] 1. The denitrification performance of Pseudomonas aeruginosa CP1 under different carbon sources

[0040] Nitrogen source (KNO 3 ) under the same condition, investigate the utilization of the strains for the carbon sources of sodium succinate, sodium citrate, glucose, sodium acetate and sucrose. The specific operation steps are as follows:

[0041] Inoculate 1mL of the bacterial suspension into 99mL of denitrification medium. The composition of the denitrification medium is as follows (g / L): KNO 3 1;Na 2 HPO 4 7.9; KH 2 PO 4 1.5; MgSO 4 ·7H 2 O 0.1; trace element solution 2mL / L; pH 7.0~7.5. Trace element solution composition (g / L): EDTA 50.0; ZnSO 4 2.2; CaCl 2 5.5; MnCl 2 4H 2 O 5.06; FeSO 4 ·7H 2 O 5.0; (NH 4 ) 6 Mo 7 o 2 4H 2 O 1.1; CuSO 4 ·5H 2 O 1.57; CoCl 2 ·6H 2 O 1.61. Add the above five carbon sources (concentrations are 9.4g / L, 6.79g / L, 4.16g / L, 5.68...

Embodiment 3

[0059] Example 3: No-current flue gas denitrification experiment of Pseudomonas aeruginosa CP1

[0060] The experiment of removing NO under high temperature environment was carried out by using the biotrickling filter tower. biofiltration systems such as Figure 8 . The nutrient composition in the liquid storage tank of the biofiltration tower during the film formation period is the same as the denitrification medium in Example 2, and the bacteria are inoculated into the biotrickling filter tower to investigate the effect of removing NOx in the flue gas under long-term operation conditions. . First, put the expanded cultured strain CP1 into the storage bottle of the circulating fluid. The film-hanging process adopts the method of intermittently soaking the filler, which includes two methods: the circulating liquid is transported to the top of the tower through the pump, and then returns to the liquid storage bottle after passing through the entire trickling filter tower, an...

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Abstract

The invention discloses a pseudomonas aeruginosa and an application. The pseudomonas aeruginosa CP1 is collected by China Centre for Type Culture Collection, wherein the collection number is CCTCC NO: 2015197, and the collection date is April 6, 2015. The strain is used for a denitrification treatment for nitrogen-containing wastewater or used for an aerobic denitrification denitration treatment for NOx smoke or waste gas. In case of no impressed current, the optimal culture condition of the strain comprises a temperature of 30-40 DEG C and C / N of not less than 12. The strain is still capable of maintaining an efficient nitrate degradation process under an aerobic condition. However, the strain is capable of achieving a removal rate of 99.01% or above for nitrate nitrogen with an initial concentration of 250mg / L within 16 hours in the case that the C / N is reduced to 4-6 under the current simulation of 10mA, and hardly generates nitrate accumulation.

Description

technical field [0001] The invention belongs to the field of environmental engineering and biology, and relates to a Pseudomonas aeruginosa (pseudomonas aeruginosa) CP1 strain with electroactive aerobic denitrification performance and its ability to remove nitrate and nitrate in water in high-temperature environment Application of nitrite. Background technique [0002] my country is a large agricultural country, and the amount of agricultural waste generated every year is huge. For a long time, my country has focused on pollution control in cities, but neglected the vast rural areas that account for 90% of the country's total area. A large number of crop wastes are randomly burned or naturally rotting, which increases the air pollution index and affects traffic and air transportation. Seeps into the ground, causing nitrate pollution of surface and ground water. On the one hand, it affects the output and quality of agricultural products; on the other hand, it also restrict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34B01D53/84B01D53/56C12R1/385C02F101/16
CPCB01D53/56B01D53/84C02F3/02C02F3/34C02F2101/16C12N1/205C12R2001/385Y02W10/10Y02A50/20
Inventor 黄少斌周少锋张永清
Owner SOUTH CHINA UNIV OF TECH
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