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Pseudomonas aeruginosa bacterial strain capable of producing phytocidal active substance and crude toxin preparation method of pseudomonas aeruginosa bacterial strain

A Pseudomonas aeruginosa, crude toxin technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve undiscovered problems

Inactive Publication Date: 2013-10-23
董金皋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through searching, we have not found relevant literature reports on using Pseudomonas aeruginosa extracts as herbicides

Method used

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  • Pseudomonas aeruginosa bacterial strain capable of producing phytocidal active substance and crude toxin preparation method of pseudomonas aeruginosa bacterial strain
  • Pseudomonas aeruginosa bacterial strain capable of producing phytocidal active substance and crude toxin preparation method of pseudomonas aeruginosa bacterial strain
  • Pseudomonas aeruginosa bacterial strain capable of producing phytocidal active substance and crude toxin preparation method of pseudomonas aeruginosa bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Dilute the crude toxin of Pseudomonas aeruginosa strain (CB-4) with distilled water to 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100mg L -1 , each adding 1% Tween 80 as an auxiliary agent, the herbicidal biological activity was determined by the stem and leaf treatment method. The specific method is: put 8 cm deep vermiculite in a plastic cup with a diameter of 7 cm and a height of 10 cm (with a small hole at the bottom of the cup), sprinkle evenly germinated crabgrass seeds in each cup, cover the vermiculite after 1 cm, and put the vermiculite after sowing. Transfer the plastic cup to the disk, keep a 0.5cm water layer, cultivate under the conditions of 25°C, 3800Lx light, and 70% humidity, until the crabgrass grows to about 2-3cm in 2 leaves for the experiment. Throat sprayer spray each agent evenly, spray 0.4mL per pot of crabgrass, observe the results after 48 hours of treatment, and calculate the mortality rate according to the following formula. The death rat...

example 2

[0024] Dissolve the crude toxin of Pseudomonas aeruginosa strain (CB-4) with methanol, and dilute it to 62.5, 125, 250, 375, 500, 750, 1000mg L -1 , Determination of herbicidal biological activity by inhibition of seed germination. The specific method is: put a piece of filter paper the size of the bottom of the dish in a φ5cm petri dish, add 2 mL of methanol solution of crude toxin of CB-4 strain of different concentrations to each dish, and add 2 mL of distilled water to each dish after it is completely dry in a ventilated place. Sow 10 germinated crabgrass seeds, and use methanol as a control. After 48 hours, measure the root shoot length, and calculate the inhibition rate according to the following formula. The inhibition rate is shown in Table 2

[0025]

[0026] Table 2 Inhibitory effect of crude toxins of different concentrations of Pseudomonas aeruginosa strain (CB-4) on crabgrass seed germination

[0027]

[0028] The IC50 of the crude toxin to inhibit the ro...

example 3

[0030] In recent years, Chlorella pyrenoidosa is often used as a model organism for herbicide screening. 250mL Erlenmeyer flask SE medium was sterilized with 100mL SE medium, inoculated with 1mL Chlorella pyrenoidosa seed solution (purchased from the Freshwater Algae Species Bank of Chinese Academy of Sciences), and 0.2mL each of crude toxin methanol solutions of different concentrations were added to make the algae solution Crude toxin concentrations were 500, 50, 10, 5 mg·L -1 , set methanol as control, light 3700Lx, culture at 25°C for 7 days, and measure the absorbance of the algae liquid with a UV spectrophotometer at the maximum absorption wavelength of 680nm every day. The results are shown in Table 3.

[0031] Table 3 different concentrations of Pseudomonas aeruginosa strain (CB-4) crude toxin treatment Chlorella pyrenoidosa

[0032]

[0033] It can be seen that with the growth of time, the inhibitory effect of the crude toxin of CB-4 strain on Chlorella pyrenoido...

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Abstract

The invention relates to a pseudomonas aeruginosa bacterial strain (serial number being CB-4) and a crude toxin preparation method thereof, which belong to the field of applying microorganisms to agricultural plant protection. The strain crude toxin has a strong inhibition effect for weeds including crab grass, amaranthus retroflexus, barnyard grass, green bristlegrass, rhodes grass and the like, and in particular respectively has IC50 reaching 299.25mg.L<-1> and 210.11mg.L<-> to seed germination root stalk length, and has the characteristics of being safe and environment-friendly, harmless to crops and the like.

Description

technical field [0001] The invention relates to a method for controlling weeds by a Pseudomonas aeruginosa strain (numbered CB-4) and its secondary metabolites, belonging to the field of microorganisms applied to agricultural plant protection. Background technique [0002] There are more than 30,000 species of weeds in the world, of which more than 1,800 species can cause damage to various agricultural crops, causing a loss of about 9.7% of the total crop output every year. Chemical herbicides are commonly used to control weeds at present, but their disadvantages are becoming more and more prominent after long-term use, such as resistance of weeds, pollution of water and soil, and difficult degradation of residues. It has become a top priority to develop new herbicides that are pollution-free and safe. Biological herbicides are favored by people because of their small impact on plants other than target weeds, small environmental negative effects, and high safety. unique ad...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P13/02C12P1/04C12R1/385
Inventor 曹宏哲董金皋张利辉徐鹏张金林
Owner 董金皋
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