Method and kit for performing quick co-detection on anti-human Hi (Haemophilus influenzae) IgM (Immunoglobulin M) and IgG (Immunoglobulin G) antibodies based on magnetic separation and multi-color quantum dot labeling

Abstract

The invention discloses a method and a kit for performing quick co-detection on anti-human Hi (Haemophilus influenzae) IgM (Immunoglobulin M) and IgG (Immunoglobulin G) antibodies based on magnetic separation and multi-color quantum dot labeling. The kit consists of anti-human Hi antibody capturing nano magnetic beads with an anti-human Hi IgM and IgG antibody gathering function, anti-human IgM and IgG antibody nano probes labeled by multi-color quantum dots, quality control substances and a PBST buffering solution, wherein the quality control substances comprise a positive quality control substance and a negative quality control substance; the positive quality control substance is serum, in which anti-human Hi IgM and IgG antibodies of human Hi infected people are respectively positive; the negative quality control substance is serum, in which anti-human Hi IgM and IgG are respectively negative. The kit and the method have the advantages of simplicity, quickness and high sensitivity, and can be used for carrying out synchronous detection on the anti-human Hi IgM and IgG antibodies.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a detection method and detection kit for rapid co-detection of anti-Haemophilus influenzae IgM and IgG antibodies based on magnetic separation and multicolor quantum dot labeling, and the preparation of the detection kit and how to use it. Background technique [0002] Haemophilus influenzae (Haemophilus influenzae, Hi) is an important respiratory pathogenic microorganism that infects humans. The bacterium was discovered by Polish bacteriologist Dr. Feffer in an influenza plague in 1892. been widely studied by researchers. Only humans are known to be the host of the pathogen. The elderly and children with poor immunity are susceptible groups, especially infants and young children under 5 years old. Hi can cause pneumonia, conjunctivitis, otitis media, meningitis and bacteremia, etc., and at least 3 million severe cases occur every year in the world, which can cause d...

AI Technical Summary

Problems solved by technology

The specificity and sensitivity of the cold agglutination test are the lowest, so its clinical diagnostic value is not great; although the gold-labeled immunoblot method and the gold-labeled immunochromatography method are simple and quick to operate, their sensitivity is slightly low, and they are not suitable for those with low antibody levels. Patients may miss the diagnosis; the preparation and operation of gelatin agglutination test reagents are cumbersome and require professional operators

Enzyme-linked immunosorbent assay has the advantages of specificity and sensitivity, and has been used to check various antibodies or antigens. However, this method has the following problems: (1) There are many steps in each batch of tests, such as adding samples, warming baths, and washing plates. It is cumbersome and takes a long time (2-4 hours in total); (2) The color components A and B need to be added separately during the detection, and the reading must be completed within the specified time, which increases labor intensity and operation to a certain extent. Possibility of mistakes; (3) This method cannot achieve simultaneous detection of IgM and IgG antibodies

Although individual detection of IgM and IgG antibodies and comprehensive analysis of the test results have no effect on the diagnosis of the disease, the operation process is cumbersome, the detection is not convenient and fast, and the cost of detection will increase compared with the joint detection

Images