Multifunctional agricultural soil remediation bacterium agent as well as preparation method and application thereof

A soil remediation and multi-functional technology, applied in the restoration of contaminated soil, biochemical equipment and methods, and microbial-based methods, can solve the problems of biological activity and single function, improve soil quality, increase available nitrogen and phosphorus Potassium content, the effect of easy operation

Inactive Publication Date: 2018-05-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that in the existing DBP microbial degradation method, microorganisms are easily affected by soil environmental factors such as indigenous microbial competition and soil temperature, and it is difficult to maintain high biological activity and relatively single function, and provide a multifunctional agricultural soil Restoration bacteria agent and its preparation method and application

Method used

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  • Multifunctional agricultural soil remediation bacterium agent as well as preparation method and application thereof
  • Multifunctional agricultural soil remediation bacterium agent as well as preparation method and application thereof
  • Multifunctional agricultural soil remediation bacterium agent as well as preparation method and application thereof

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specific Embodiment approach 1

[0047] Specific embodiment 1: The multifunctional agricultural soil remediation bacterial agent of this embodiment includes microbial agent A and DBP degrading bacteria fermentation liquid, and said microbial agent A includes sphingolipid bacteria fermentation liquid, colloid bacillus fermentation liquid and acid-producing gram Lebsiella fermentation broth.

[0048] The DBP-degrading bacterium is Pseudomonas sp. DNB-S1, which is preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing , the date of deposit is November 6, 2014, and the deposit number is CGMCC No.9526. It has been disclosed in the patent 201410798113.0.

[0049] The bacterial agent of this embodiment can improve the fertility of the polluted soil while repairing the DBP polluted soil, which is of great significance for the clean production of agriculture, ...

specific Embodiment approach 2

[0050] Embodiment 2: This embodiment differs from Embodiment 1 in that the mass ratio of the microbial agent A to the fermentation broth of the DBP-degrading bacteria is (5-10): (2-4). Others are the same as in the first embodiment.

specific Embodiment approach 3

[0051] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the mass ratio of sphingomyces fermented liquid, colloid bacillus fermented liquid and oxytoca klebsiella fermented liquid is ( 1-2):(1-2):(5-10). Others are the same as in the first embodiment.

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Abstract

The invention provides a multifunctional agricultural soil remediation bacterium agent as well as a preparation method and application thereof, and relates to a bacterium agent as well as a preparation method and application thereof, aiming at solving the problems of an existing microorganism degradation method of DBP (Dibutyl Phthalate) that microorganisms are easily influenced by soil environment factors including indigenous microorganism competition, soil temperature and the like, relatively high bioactivity is difficult to keep and the function is relatively single. The bacterium agent isprepared from a microorganism bacterium agent A and a DBP degradation bacterium fermentation solution, wherein the microorganism bacterium agent A is prepared from a sphingobium fermentation solution,a bacillus mucilaginosus fermentation solution and a klebsiella oxytoca fermentation solution. The method comprises the following steps: preparing the microorganism bacterium agent A; preparing the DBP degradation bacterium fermentation solution; mixing the DBP degradation bacterium fermentation solution with the microorganism bacterium agent A to obtain the multifunctional agricultural soil remediation bacterium agent. The bacterium agent provided by the invention can keep relatively high biological remediation activity in a soil environment for a long term, and the remediation efficiency ofDBP pollution is effectively improved; when DBP polluted soil is repaired, the fertility of the polluted soil also can be improved. The multifunctional agricultural soil remediation bacterium agent is used for the field of soil remediation.

Description

technical field [0001] The invention relates to a bacterial agent and its preparation method and application. Background technique [0002] The rapid development of agriculture has led to the extensive use of agricultural mulch, and the use of agricultural mulch in China ranks first in the world, and the situation of white pollution caused by agricultural mulch is severe. Dibutyl phthalate (DBP) is an important phthalate ester (PAEs) compound, as a plasticizer, it is widely used in plastic agricultural film. The extensive use of agricultural film has led to serious DBP pollution in agricultural soil in my country. DBP pollution will not only lead to deterioration of soil quality and affect agricultural production, but DBP will also accumulate in crops and enter the human body through the food chain, causing damage to human endocrine, nervous system, etc. Harm. At present, both China National Environmental Testing Center and the US Environmental Protection Agency (EPA) list ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/07C12R1/22
CPCB09C1/10C12N1/20
Inventor 张颖王蕾付嘉伟董俊伟于晖陶月林宇龙姜昭冯程程曹博孙瑞雪
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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