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Preparation method and application of microbial flocculant and application of related strains in preparing microbial flocculant

A technology for microbial flocculants and strains is applied in the application field of preparing microbial flocculants, which can solve the problems of destroying the natural ecology of water bodies, serious threats to aquaculture, affecting agricultural production, etc. The effect of sedimentation and flocculation

Inactive Publication Date: 2016-04-27
虞磊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Acid-base wastewater is the most common type of wastewater treatment. It comes from steel plants, chemical plants, dye factories, electroplating plants, and mines. These industries are the economic lifelines of various countries. They are widely distributed and have a large amount of sewage. In industrial wastewater It accounts for a large proportion. If it is discharged without treatment, it will corrode pipes, channels and hydraulic structures; after it is discharged into the water body, it will change the pH of the water body, destroy the natural ecology of the water body, and pose a serious threat to the aquaculture industry; it will easily cause pollution if it penetrates into the soil. Affect agricultural production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of this microbial flocculant, its step comprises:

[0021] (1) Use a pipette gun to transfer 100 μL of Klebsiellaoxytoca GS-4-08 bacterial solution from the strain storage tube to the triangular flask containing the strain activation medium, and shake it in a shaker with a set temperature of 35°C and a rotational speed of 150 rpm After 12 hours, use an inoculation loop to dip a small amount of bacterial solution and draw a line on the activated plate medium, then place it in the incubator, cultivate it at 35°C for 24 hours, and use the inoculation loop to pick the strains on the activated plate medium for inoculation. Put it into the Erlenmeyer flask containing the culture medium, and shake it for 24 hours in a shaker with a set temperature of 35°C and a rotation speed of 150rpm;

[0022] The components of the strain activation medium are: peptone 10g, yeast extract 5g, sodium chloride 10g, add water to 1L, pH7;

[0023] The components of the act...

Embodiment 2

[0028] The application of the microbial flocculant in the treatment of iron-containing acidic wastewater comprises the following steps:

[0029] Add 0.2mg of microbial flocculant to 50ml of iron-containing kaolin suspension at a concentration of 4g / L, let stand for 10min after stirring, and measure the 550nm absorbance value of the supernatant, wherein the iron concentration of the kaolin suspension is 3mM, and the pH value It is about 3, and the reaction temperatures are 4, 25, 35, 45, 55, 65, and 75°C. The experimental results are shown in Table 1.

Embodiment 3

[0031] The application of the microbial flocculant in the treatment of iron-containing acidic wastewater comprises the following steps:

[0032] Add 0.2 mg of microbial flocculant to 50 ml of iron-containing kaolin suspension at a concentration of 4 g / L, let it stand for 10 minutes after stirring, and measure the 550 nm absorbance value of the supernatant, wherein the pH value of the kaolin suspension is about 3, and the reaction The temperature was 25°C, and the iron contents were 0.5mM, 1mM, 1.5mM, 2mM, 2.5mM, and 3mM. The experimental results are shown in Table 2.

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Abstract

The invention discloses a preparation method of a microbial flocculant. The preparation method comprises the following steps: culturing a Klebsiella oxytoca GS-4-08 strain in a fermentation culture solution; centrifuging the fermentation culture solution so as to obtain a supernatant, precipitating the supernatant with the addition of ethanol, and collecting a precipitate; dissolving the precipitate in pure water, dialyzing and freeze-drying so as to obtain the microbial flocculant in powder form. The invention also discloses application of the microbial flocculant in iron-containing acid wastewater treatment. The invention also discloses application of the Klebsiella oxytoca GS-4-08 strain in preparing the microbial flocculant. The microbial flocculant disclosed by the invention has the beneficial effects that the microbial flocculant is relatively high in yield, good in flocculating effect and broad in treatment range, and the microbial flocculant is capable of achieving good settling and flocculating effects on iron-containing acid wastewater; and the method is simple to operate and cheap in raw material, and the method is capable of significantly saving dosage.

Description

technical field [0001] The invention relates to a preparation method of a microbial flocculant and its application in the treatment of iron-containing acidic wastewater, as well as the application of KlebsiellaoxytocaGS-4-08 strains in the preparation of the microbial flocculant, belonging to the technical field of industrial wastewater treatment. Background technique [0002] As a water treatment agent used earlier, chemical flocculants mainly include inorganic flocculants and organic polymer flocculants. These flocculants are cheap and have obvious treatment effects, but they are not easy to degrade. Extensive use will lead to residual sludge that is difficult to degrade. A large amount of enrichment causes secondary pollution. Compared with chemical flocculants, microbial flocculants are high-molecular organic substances with flocculation function produced by microorganisms. The main components are polysaccharides and proteins, which are easy to biodegrade, have little env...

Claims

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Application Information

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IPC IPC(8): C12P1/04C02F3/34C12R1/22C02F101/10
Inventor 虞磊汤晴文王时曹明月袁坤李佳
Owner 虞磊
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